Abstract
SOME of the subunits of yeast cytochrome oxidase, cytochrome b and ATPase are synthesised on mitochondrial ribosomes. Inhibition of protein synthesis by antibiotics that specifically affect mitochondrial ribosomes leads to the absence of three subunits of cytochrome oxidase, at least three subunits of ATPase, and a 30,000 molecular weight polypeptide believed to be cytochrome b (ref. 1). This indicated that the mitochondrion is the site of synthesis of these polypeptides, but only inferences could be drawn as to whether their mRNAs were transcribed from mitochondrial DNA (mDNA) or from nuclear DNA. We report here the isolation of a temperature-sensitive mutant of Saccharomyces cerevisiae which is mitochondrially inherited and has an altered subunit of cytochrome c oxidase. The simplest interpretation of our results is that mDNA codes for this subunit.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Schatz, G. & Mason, T. L. A. Rev. Biochem. 43, 51–88 (1974).
Tzagoloff, A., Akai, A., Needleman, R. B. & Zulch, G. J. biol. Chem. 250, 8236–8242 (1975).
Slonimski, P. P. & Tzagoloff, A. Eur. J. Biochem. 61, 27–41 (1976).
Putrament, A., Baranowska, H. & Prazmo, W. Molec. gen. Genet. 126, 357–366 (1973).
Henry, S. A., Donahue, T. F. & Culbertson, M. R. Molec. gen. Genet. 143, 5–11 (1975).
Tzagoloff, A., Akai, A. & Needleman, R. B. J. Bact. 122, 826–831 (1975).
Lowry, O. H., Rosebrough, H. J., Farr, A. L. & Randall, R. J. J. biol. Chem. 93, 265–275 (1951).
Tzagoloff, A., Akai, A. & Rubin, M. S. in The Biogenesis of Mitochondria (eds Kroon, A. M. & Saccone, C.), 405–421 (Academic, New York, 1974).
Douglas, W. G. & Butow, R. A. Proc. natn. Acad. Sci. U.S.A. 73, 1083–1086 (1976).
Needleman, R. B. & Tzagoloff, A. Analyt. Biochem. 64, 545–549 (1975).
Rubin, M. S. & Tzagoloff, A. J. biol. Chem. 248, 4269–4274 (1973).
Horwitz, M. S. & Scharff, M. D. in Fundamental Techniques in Virology 297–315 (Academic New York, 1969).
Wu, G. J. & Bruening, G. Virology 46, 596–612 (1971).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
ECCLESHALL, T., NEEDLEMAN, R., BUCHFERER, B. et al. A temperature-sensitive yeast mitochondrial mutant with altered cytochrome c oxidase subunit. Nature 273, 67–70 (1978). https://doi.org/10.1038/273067b0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/273067b0
This article is cited by
-
Mapping of mitochondrial DNA from Torulopsis glabrata
Current Genetics (1980)
-
Fine structure of OXI1, the mitochondrial gene coding for subunit II of yeast cytochrome c oxidase
Current Genetics (1979)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
