Abstract
NORMAL antibacterial activity in the lung is largely dependent on alveolar macrophage function1. Products of antigen-stimulated, sensitised lymphocytes can activate alveolar macrophages, which then exhibit greater adherence, phagocytosis and nonspecific bactericidal capacity than normal2. Although the molecular basis of this activation is unknown, influx or intracellular redistribution of divalent cations (Ca2+ and Mg2+) has been implicated in other cell types3–10. We have therefore examined the effect on alveolar macrophage function of exposure to the ionophore A23187, which selectively binds divalent cations at neutral pH and passively transfers them across biological membranes11,12.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Green, G. M. A. Rev. Med. 19, 315–336 (1968).
Johnson, J. D., Hand, W. L., King, N. L., and Hughes, C. G., J. Immun., 115, 80–84 (1975).
Steinhardt, R. A., Epel, D., Carroll, E. J., Jr, and Yanagimachi, R., Nature, 252, 41–43 (1974).
Alford, R. H. J. Immun. 104, 698–703 (1970).
Maino, V. C., Green, N. M., and Crumpton, M. J., Nature, 251, 324–327 (1974).
Luckasen, J. R., White, J. G., and Kersey, J. H., Proc. natn. Acad. Sci. U.S.A., 71, 5088–5090 (1974).
Freedman, M. H., Raff, M. C., and Gomperts, B., Nature, 255, 378–382 (1975).
Balk, S. D., Whitfield, J. F., Youdale, T., and Braun, A. C., Proc. natn. Acad. Sci. U.S.A., 70, 675–679 (1973).
Rubin, H. Proc. natn. Acad. Sci. U.S.A., 72, 3551–3555 (1975).
Berridge, M. J., J. Cyclic Nucleotide Res., 1, 305–320 (1975).
Reed, P. W., and Lardy, H. A., in The Role of Membranes in Metabolic Regulation (edit. by Mehlman, M. A., and Hanson, R. W.), 111–131 (Academic, New York, 1972).
Reed, P. W., and Lardy, H. A., J. biol. Chem., 247, 6970–6977 (1972).
Cantey, J. R., and Hand, W. L., J. clin. Invest., 54, 1125–1134 (1974).
Axline, S. G., J. exp. Med., 128, 1031–1048 (1968).
Bryant, R. E., and Jenkins, D. E., Jr, J. Immun., 101, 664–668 (1968).
Mackaness, G. B., Semin. Hematol., 7, 172–184 (1970).
Schultz, G., Hardman, J. G., Schultz, K., Baird, C. E., and Sutherland, E. W., Proc. natn. Acad. Sci. U.S.A., 70, 3889–3893 (1973).
Whitfield, J. F., MacManus, J. P., Bovnton, A. L., Gillan, D. J., and Isaacs, R. J. J. Cell Physiol., 84, 445–457 (1974).
Hadden, J. W., Hadden, E. M., Haddox, M. K., and Goldberg, N. D., Proc. natn. Acad. Sci. U.S.A., 69, 3024–3027 (1972).
Goldberg, N. D., O'Dea, R. F., and Haddox, M. K., in Advance in Cyclic Nucleotide Research, 3 (edit. by Greengard, P., and Robinson, G. A.), 155–223 (Raven, New York, 1973).
Seifert, W. E., and Rudland, P. S., Nature, 248, 138–140 (1974).
Weinstein, Y., Chambers, D. A., Bourne, H. R., and Melmon, K. L. Nature, 251, 352–353 (1974).
Watson, J., J. exp. Med., 141, 97–111 (1975).
Rubin, H., and Koide, T., Proc. natn. Acad. Sci. U.S.A., 73, 168–172 (1976).
Pfeiffer, D. R., and Lardy, H. A., Biochemistry, 15, 935–943 (1976).
Johnson, J. D., Paul, M., and Epel, D., Nature, 262, 661–664 (1976).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
HAND, W., KING, N., JOHNSON, J. et al. Requirement for magnesium influx in activation of alveolar macrophages mediated by ionophore A23187. Nature 265, 543–544 (1977). https://doi.org/10.1038/265543a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/265543a0
This article is cited by
-
Lymphokine-induced macrophage aggregation: involvement of cyclic-GMP and microtubules
Agents and Actions (1981)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.