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Corrections in the catalogue of oligonucleotides produced by digestion of Escherichia coli 16S rRNA with T1 RNase

Abstract

SINCE its initial publication1, the catalogue of oligonucleotides generated by T1 RNase digestion of Escherichia coli 16S RNA has undergone a number of revisions and refinements2–5. At present the literature contains two rather dissimilar documented versions of this catalogue2,3 and disagreements hold for 50% of sequences octamer and larger3. Confusion is not relieved by recent publications concerning the 16SrRNA primary structure6,7 that now incorporate some of the oligomer sequences of Uchida et al.3 but reject others, without resort to new evidence, however.

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MAGRUM, L., ZABLEN, L., STAHL, D. et al. Corrections in the catalogue of oligonucleotides produced by digestion of Escherichia coli 16S rRNA with T1 RNase. Nature 257, 423–426 (1975). https://doi.org/10.1038/257423a0

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