Abstract
SEVERAL peptidases which are active around neutral pH have been described in polymorphonuclear (PMN) leucocytes. One of these, which we have found concentrated within the lysosomes of rabbit peritoneal exudate PMN cells, hydrolyses histone but not haemoglobin at neutral pH1,2. Here we compare the subcellular distribution of this enzyme with that of three peptidases active at near-neutral pH in rabbit PMN cells. Hydrolysis of their respective substrates seems to be due to separate activities, and this distinction must precede any attempts to ascribe a function to the enzymes in tissue injury3–6.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Davies, D. T. P., and Weissmann, G., J. Cell Biol., 43, 29a (1969).
Davies, D. T. P., Krakauer, K., and Weissmann, G., Fed. Proc., 29, 784 (1970).
Reekallio, J., and Makinen, P. L., Experientia, 25, 929 (1969).
Becker, E. L., and Ward, P. A., J. Exp. Med., 129, 569 (1969).
Janoff, A., Biochem. J., 114, 157 (1969).
Janoff, A., and Scherer, J., J. Exp. Med., 123, 1137 (1968).
Cohn, Z. A., and Hirsch, J. G., J. Exp. Med., 112, 983 (1960).
Weissmann, G., Spilberg, I., and Krakauer, K., Arth. Rheum., 12, 103 (1969).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1951).
Talalay, P., Fishman, W., and Huggins, C., J. Biol. Chem., 166, 757 (1946).
Baggiolini, M., Hirsch, J. C., and de Duve, C., J. Cell. Biol., 40, 529 (1969).
Barrett, A. J., in Lysosomes in Biology and Pathology (edit. by Dingle, J. T., and Fell, H. B.) 2, 245 (1969).
Mahadevan, S., and Tappel, A. L., J. Biol. Chem., 242, 2369 (1967).
Sylven, B., and Snellman, O., Histochemie, 12, 240 (1968).
Snellman, O., Biochem.J., 114, 673 (1969).
Wasl, S., Murray, R. K., Macmorine, D. R. L., and Movat, H. Z., Brit. J. Exp. Path., 47, 411 (1966).
Barrett, A. J., and Poole, A. R., Nature, 224, 279 (1969).
McDonald, J. K., Zeitman, B. B., and Ellis, S., Nature, 225, 1048 (1970).
Visser, L., and Blout, E. R. Fed. Proc., 28, 407 (1969).
Chayen, J., Bitensky, L., Butcher, R. C., and Poulter, L. W., Nature, 222, 281 (1969).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
DAVIES, P., KRAKAUER, K. & WEISSMANN, G. Subcellular Distribution of Neutral Protease and Peptidases in Rabbit Polymorphonuclear Leucocytes. Nature 228, 761–762 (1970). https://doi.org/10.1038/228761a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/228761a0
This article is cited by
-
Granule enzymes of human and rabbit polymorphonuclear leukocytes An investigation of enzyme solubility
Inflammation (1987)
-
Inhibition of synovial fluid lysosomal glycosidases by anti-Arthritic gold preparations
Agents and Actions (1978)
-
Changes in cellular enzyme levels and the inhibition of selective release of lysosomal hydrolases from macrophages by indomethacin
Agents and Actions (1975)
-
Effect of Cyclic AMP on Release of Lysosomal Enzymes from Phagocytes
Nature New Biology (1971)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
