Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Purification and Properties of Initiation Factor F3

Nature volume 228, pages 1269–1273 (1970)Cite this article

Abstract

Homogeneous preparations of initiation factor F3 have both RNA binding and ribosome dissociation activities. But although DF activity could entirely account for the role of F3 in the ribosome cycle this factor has an additional function in formation of the initiation complex that may be related to its RNA binding properties.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Miller, M. J., Zasloff, M., and Ochoa, S., FEBS Lett., 3, 50 (1969).

    Article  CAS  PubMed  Google Scholar 

  2. Parenti-Rosina, R., Eisenstadt, A., and Eisenstadt, J. M., Nature, 221, 363 (1969).

    Article  ADS  CAS  PubMed  Google Scholar 

  3. Iwasaki, K., Sabol, S., Wahba, A. J., and Ochoa, S., Arch. Biochem. Biophys., 125, 542 (1968).

    Article  CAS  PubMed  Google Scholar 

  4. Revel, M., Herzberg, M., Becarevic, A., and Gros, F., J. Mol. Biol., 33, 231 (1968).

    Article  CAS  PubMed  Google Scholar 

  5. Mazumder, R., Chae, Y-B., and Ochoa, S., Proc. US Nat. Acad. Sci., 63, 98 (1969).

    Article  ADS  CAS  Google Scholar 

  6. Chae, Y-B., Mazumder, R., and Ochoa, S., Proc. US Nat. Acad. Sci., 63, 828 (1969).

    Article  ADS  CAS  Google Scholar 

  7. Kolakofsky, D., Dewey, K. F., Hershey, J. W. B., and Thach, R. E., Proc. US Nat. Acad. Sci., 61, 1066 (1968).

    Article  ADS  CAS  Google Scholar 

  8. Lelong, J. C., Grunberg-Manago, M., Dondon, J., Gros, D., and Gros, F., Nature, 226, 505 (1970).

    Article  ADS  CAS  PubMed  Google Scholar 

  9. Chae, Y-B., Mazumder, R., and Ochoa, S., Pros. US Nat. Acad. Sci., 62, 1181 (1969).

    Article  ADS  CAS  Google Scholar 

  10. Hershey, J. W. B., Dewey, K. F., and Thach, R. E., Nature, 222, 944 (1969).

    Article  ADS  CAS  PubMed  Google Scholar 

  11. Subramanian, A. R., Davis, B. D., and Beller, R. J., Cold Spring Harbor Symp. Quant. Biol., 34, 223 (1969).

    Article  CAS  PubMed  Google Scholar 

  12. Nomura, M., and Lowry, C. V., Proc. US Nat. Acad. Sci., 58, 946 (1967).

    Article  ADS  CAS  Google Scholar 

  13. Schlessinger, D., Mangiarotti, G., and Apirion, D., Proc. US Nat. Acad. Sci., 58, 1782 (1967).

    Article  ADS  CAS  Google Scholar 

  14. Kaempfer, R., Proc. US Nat. Acad. Sci., 61, 106 (1968).

    Article  ADS  CAS  Google Scholar 

  15. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1951).

    CAS  PubMed  Google Scholar 

  16. Weber, K., and Osborn, M., J. Biol. Chem., 244, 4406 (1969).

    CAS  PubMed  Google Scholar 

  17. Cannata, J. J. B., Focesi, jun., A., Mazumder, R., Warner, R. C., and Ochoa, S., J. Biol. Chem., 240, 3249 (1965).

    CAS  PubMed  Google Scholar 

  18. Shapiro, A. L., Viñuela, E., and Maizell, J. V., Biochem. Biophys. Res. Commun., 28, 815 (1967).

    Article  CAS  PubMed  Google Scholar 

  19. Ellman, G. L., Arch. Biochem. Biophys., 82, 70 (1959).

    Article  CAS  PubMed  Google Scholar 

  20. Brown, J. C., and Doty, P., Biochem. Biophys. Res. Commun., 30, 284 (1968).

    Article  CAS  PubMed  Google Scholar 

  21. Kohler, R. W., Ron, E. Z., and Davis, B. D., J. Mol. Biol., 36, 71 (1968).

    Article  CAS  PubMed  Google Scholar 

  22. Subramanian, A. R., Ron, E. Z., and Davis, B. D., Proc. US Nat. Acad. Sci., 61, 761 (1968).

    Article  ADS  CAS  Google Scholar 

  23. Miller, M. J., thesis, New York University (1969).

  24. Gonzalez, N. S., Bade, E. G., and Algranati, I. D., FEBS Lett., 4, 331 (1969).

    Article  CAS  PubMed  Google Scholar 

  25. Albrecht, J., Stap, F., Voorma, H. O., van Knippenberg, P. H., and Bosch, L., FEBS Lett., 6, 297 (1970); Albrecht, J., thesis, Univ. Leiden (1970).

    Article  CAS  PubMed  Google Scholar 

  26. Miall, S. H., Kato, T., and Tamaoki, T., Nature, 226, 1050 (1970).

    Article  ADS  CAS  PubMed  Google Scholar 

  27. McLaughlin, C. S., Dondon, J., Grunberg-Manago, M., Michelson, A. M., and Saunders, G., J. Mol. Biol., 32, 521 (1968).

    Article  CAS  PubMed  Google Scholar 

  28. Kondo, M., Eggerston, G., Eisenstadt, J., and Lengyel, F., Nature, 220, 368 (1968).

    Article  ADS  CAS  PubMed  Google Scholar 

  29. Ghosh, H. P., and Khorana, H. G., Proc. US Nat. Acad. Sci., 58, 2455 (1967).

    Article  ADS  CAS  Google Scholar 

  30. Nirenberg, M. W., and Leder, P., Science, 145, 1399 (1964).

    Article  ADS  CAS  PubMed  Google Scholar 

  31. Algranati, I. D., Gonzalez, N. S., and Bade, E. G., Proc. US Nat. Acad. Sci, 62, 574 (1969).

    Article  ADS  CAS  Google Scholar 

  32. Friedman, H., Lu, P., and Rich, A., Nature, 223, 909 (1969).

    Article  ADS  CAS  PubMed  Google Scholar 

  33. Revel, M., Lelong, J. C., Brawerman, G., and Gros, F., Nature, 219, 1016 (1968).

    Article  ADS  CAS  PubMed  Google Scholar 

  34. Greenshpan, H., and Revel, M., Nature, 224, 331 (1969).

    Article  ADS  CAS  PubMed  Google Scholar 

Download references

Author information

Author notes

  1. MARIA ANTONIA G. SILLERO

    Present address: Institute de Enzimología, Centro de Investigaciones Biológicas, C.S.I.C., Madrid

  2. KENTARO IWASAKI

    Present address: Institute of Medical Science, University of Tokyo, Takanawa, Minatoku, Tokyo

Authors and Affiliations

  1. Department of Biochemistry, New York University School of Medicine, New York, 10016

    STEVEN SABOL, MARIA ANTONIA G. SILLERO, KENTARO IWASAKI & SEVERO OCHOA

Authors
  1. STEVEN SABOL
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. MARIA ANTONIA G. SILLERO
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. KENTARO IWASAKI
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. SEVERO OCHOA
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

SABOL, S., SILLERO, M., IWASAKI, K. et al. Purification and Properties of Initiation Factor F3. Nature 228, 1269–1273 (1970). https://doi.org/10.1038/2281269a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/2281269a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing