Abstract
Methods are developed for computing three dimensional density maps from transmission electron micrographs using Fourier transforms. The reconstructions objectively combine data from several different views of one or more particles. When applied to negatively stained spherical viruses, the technique gives clear reconstructions of their surface structures.
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References
De Rosier, D. J., and Klug, A., Nature, 217, 130 (1968).
Moore, P. B., Huxley, H. E., and De Rosier, D. J., J. Mol. Biol. (in the press).
Finch, J. T., and Gibbs, A. J., J. Gen. Virol., 6, 141 (1970).
Crowther, R. A., De Rosier, D. J., and Klug, A., Proc. Roy. Soc., A (in the press).
Caspar, D. L. D., and Klug, A., Cold Spring Harbor Symp. Quant. Biol., 27, 1 (1962).
Arndt, U. W., Crowther, R. A., and Mallett, J. F. W., J. Sci. Instrum., Series 2, 1, 510 (1968).
Klug, A., and Finch, J. T., J. Mol. Biol., 11, 403 (1965).
Klug, A., and Finch, J. T., J. Mol. Biol., 31, 1 (1968).
Klug, A., and Caspar, D. L. D., Adv. Virus Res., 7, 225 (1960).
Finch, J. T., Klug, A., and Leberman, R., J. Mol. Biol. (in the press).
Harrison, S. C., thesis, Harvard Univ. (1967).
Cooley, J. W., and Tukey, J. W., Maths. of Computation, 19, 297 (1965).
Whittaker, E. T., Proc. Roy. Soc. Edinburgh, 35, 181 (1915); Shannon, C. E., Proc. Inst. Radio Eng. New York, 37, 10 (1949).
Sayre, D., Acta Cryst., 5, 843 (1952).
Klug, A., Crick, F. H. C., and Wyckoff, H. W., Acta Cryst., 11, 199 (1958).
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CROWTHER, R., AMOS, L., FINCH, J. et al. Three Dimensional Reconstructions of Spherical Viruses by Fourier Synthesis from Electron Micrographs. Nature 226, 421–425 (1970). https://doi.org/10.1038/226421a0
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DOI: https://doi.org/10.1038/226421a0
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