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Amino-acid Sequence of Porcine Pancreatic Elastase and its Homologies with other Serine Proteinases

Nature volume 225, pages 802–806 (1970)Cite this article

Abstract

The primary structure of porcine elastase has been determined by classical procedures of enzymatic digestion and chemical sequence analysis and shown to be closely related to those of chymotrypsins A and B and trypsin. Differences in side-chains offer an explanation of the differences in substrate specificity.

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References

  1. Lewis, U. T., Williams, D. E., and Brink, N. G., J. Biol. Chem., 222, 705 (1956).

    CAS  PubMed  Google Scholar 

  2. Partridge, S. M., and Davis, H. F., Biochem. J., 61, 21 (1955).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Naughton, M. A., and Sanger, F., Biochem. J., 78, 156 (1961).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Grant, N. H., and Robbin, K. C., Arch. Biophys. Biochem., 66, 396 (1957).

    Article  CAS  Google Scholar 

  5. Moon, H. D., and McIvor, B. C., J. Immunol., 85, 78 (1960).

    CAS  PubMed  Google Scholar 

  6. Hartley, B. S., Ann. Rev. Biochem., 29, 45 (1960).

    Article  CAS  PubMed  Google Scholar 

  7. Naughton, M. A., Sanger, F., Hartley, B. S., and Shaw, D. C., Biochem. J., 77, 149 (1960).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Smillie, L. B., and Hartley, B. S., Biochem. J., 101, 232 (1966).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Brown, J. R., Kauffman, D. L., and Hartley, B. S., Biochem. J., 103, 497 (1967).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Shotton, D. M., Hartley, B. S., Camerman, N., Hofmann, T., Nyburg, S. C., and Rao, L., J. Mol. Biol., 32, 155 (1968).

    Article  CAS  PubMed  Google Scholar 

  11. Shotton, D. M., and Watson, H. C., Nature, 225, 811 (1970). (Third article of this series.)

    Article  ADS  CAS  PubMed  Google Scholar 

  12. Gray, W. R., in Methods in Enzymology (edit. by Hirs, C. H. W.), 11, 469 (Academic Press, New York, 1967).

    Google Scholar 

  13. Offord, R. E., Nature, 21l, 591 (1966).

    Article  ADS  Google Scholar 

  14. Watson, H. C., Shotton, D. M., Cox, J. M., and Muirhead, H., Nature, 225, 806 (1970). (Second article of this series.)

    Article  ADS  CAS  PubMed  Google Scholar 

  15. Shotton, D. M., and Watson, H. C., Phil. Trans. Roy. Soc., B, 257, 111 (1970).

    Article  ADS  CAS  Google Scholar 

  16. Walsh, K. A., Kauffman, D. L., Kumar, K. S. V. S., and Neurath, H., Proc. US Nat. Acad. Sci., 51, 301 (1964).

    Article  ADS  CAS  Google Scholar 

  17. Mikeš, O., Tomašek, V., Holeyšovský, V., and Šorm, F., Biochim. Biophys. Acta, 117, 281 (1966).

    Article  PubMed  Google Scholar 

  18. Hartley, B. S., Nature, 201, 1284 (1964).

    Article  ADS  CAS  PubMed  Google Scholar 

  19. Hartley, B. S., and Kauffman, D. L., Biochem. J., 101, 229 (1966).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Meloun, B., Kluh, I., Kostka, V., Morávek, L., Prusík, Z., Vaněček, J., Keil, B., and Šorm, F., Biochim. Biophys. Acta, 130, 543 (1966).

    Article  CAS  PubMed  Google Scholar 

  21. Smillie, L. B., Furka, A., Nagabhushan, N., Stevenson, K. J., and Parkes, C. O., Nature, 218, 343 (1968).

    Article  ADS  CAS  PubMed  Google Scholar 

  22. Hartley, B. S., Phil. Trans. Roy. Soc., B, 257, 77 (1970).

    Article  ADS  CAS  Google Scholar 

  23. Blow, D. M., Birktoft, J. J., and Hartley, B. S., Nature, 221, 337 (1969).

    Article  ADS  CAS  PubMed  Google Scholar 

  24. Birktoft, J. J., Blow, D. M., Henderson, R., and Steitz, T. A., Phil. Trans. Roy. Soc., B, 257, 57 (1970).

    ADS  Google Scholar 

  25. Keller, P. J., Cohen, E., and Neurath, H., J. Biol. Chem., 233, 344 (1958).

    CAS  PubMed  Google Scholar 

  26. Brown, J. R., Greenshields, R. N., Yamasaki, M., and Neurath, H., Biochemistry, 2, 867 (1963).

    Article  CAS  PubMed  Google Scholar 

  27. Gratecos, D., Guy, O., Rovery, M., and Desnuelle, P., Biochim. Biophys. Acta, 175, 82 (1969).

    Article  CAS  PubMed  Google Scholar 

  28. Banga, I., Acta Physiol. Acad. Sci., Hung., 3, 317 (1952).

    Article  CAS  Google Scholar 

  29. Charles, M., Rovery, M., Guidoni, A., and Desnuelle, P., Biochim. Biophys. Acta, 69, 115 (1963).

    Article  CAS  PubMed  Google Scholar 

  30. Bricteux-Gregoire, S., Schyns, R., and Florkin, M., Biochim. Biophys. Acta, 127, 277 (1966).

    Article  CAS  PubMed  Google Scholar 

  31. Charles, M., Gratecos, D., Rovery, M., and Desnuelle, P., Biochim. Biophys. Acta, 140, 395 (1967).

    Article  CAS  PubMed  Google Scholar 

  32. Peanasky, R. J., Gratecos, D., Baratti, J., and Rovery, M., Biochim. Biophys. Acta, 181, 82 (1969).

    Article  CAS  PubMed  Google Scholar 

  33. Folk, J. E., and Schirmer, W. E., J. Biol. Chem., 240, 181 (1965).

    CAS  PubMed  Google Scholar 

  34. Tobita, C. T., and Folk, J. E., Abstr. Seventh Intern. Congr. Biochem., 4, 599 (1967).

    Google Scholar 

  35. Enenkel, A. G., and Smillie, L. B., Biochemistry, 2, 1449 (1963).

    Article  CAS  PubMed  Google Scholar 

  36. Matthews, B. W., Sigler, P. B., Henderson, R., and Blow, D. M., Nature, 214, 652 (1967).

    Article  ADS  CAS  PubMed  Google Scholar 

  37. Magnusson, S., Biochem. J., 110, 25P (1968).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Neurath, H., Bradshaw, R. A., and Arnon, R., Abstr. Intern. Union Biochem. Symp. Copenhagen (in the press).

  39. Barnard, E. A., and Hope, W. C., Biochim. Biophys. Acta, 178, 364 (1969).

    Article  CAS  PubMed  Google Scholar 

  40. Gibson, D., and Dixon, G. H., Nature, 222, 753 (1969).

    Article  ADS  CAS  PubMed  Google Scholar 

  41. Smillie, L. B., Abstr. Intern. Union Biochem. Symp., Copenhagen (in the press).

  42. Smillie, L. B., and Whitaker, D. R., J. Amer. Chem. Soc., 89, 3350 (1967).

    Article  CAS  Google Scholar 

  43. Sanger, F., Proc. Chem. Soc., 76 (1963).

  44. Smith, E. L., Markland, F. S., Kasper, C. B., DeLange, R. J., Landon, M., and Evans, W. H., J. Biol. Chem., 241, 5974 (1966).

    CAS  PubMed  Google Scholar 

  45. Wright, C. S., Alden, R. A., and Kraut, J., Nature, 221, 235 (1969).

    Article  ADS  CAS  PubMed  Google Scholar 

  46. Shaw, D. C., and Wells, J. R. E., Biochem. J., 104, 5C (1967).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  47. Ambler, R. P., Biochem. J., 89, 349 (1963).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  48. Hartley, B. S., Brown, J. R., Kauffman, D. L., and Smillie, L. B., Nature, 207, 1157 (1965).

    Article  ADS  CAS  PubMed  Google Scholar 

  49. Crick, F. H. C., J. Mol. Biol., 38, 367 (1968).

    Article  CAS  PubMed  Google Scholar 

  50. Shotton, D. M., in Methods in Enzymology (edit. by Perlmann, G. E., and Lorand, L.) (Academic Press, New York, in the press).

  51. Gertler, A., and Birk, Y., Europ. J. Biochem. (in the press).

  52. Geneste, P., and Bender, M. L., Biochem. Biophys. Res. Commun. (in the press).

  53. Sampath Narayanan, A., and Anwar, R. A., Biochem. J., 114, 11 (1969).

    Article  PubMed Central  Google Scholar 

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Author notes

  1. D. M. SHOTTON

    Present address: Laboratory of Molecular Enzymology, Department of Biochemistry, University of Bristol, Bristol, B58 1TD

Authors and Affiliations

  1. MRC Laboratory of Molecular Biology, University Postgraduate Medical School, Hills Road, Cambridge

    D. M. SHOTTON & B. S. HARTLEY

Authors
  1. D. M. SHOTTON
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  2. B. S. HARTLEY
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SHOTTON, D., HARTLEY, B. Amino-acid Sequence of Porcine Pancreatic Elastase and its Homologies with other Serine Proteinases. Nature 225, 802–806 (1970). https://doi.org/10.1038/225802a0

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