Abstract
DOTY et al.1 have reported that when an aqueous solution of DNA is heat-denatured and then annealed by slow cooling, the complementary strands separated by the thermal treatment recombine specifically. By this method, Marmur and Lane2 made hybrid DNA molecules in which one of the complementary strands derived from the DNA of a streptomycin-resistant strain of D. pneumoniae, and the other strand from a wild-type strain. The hybrid molecules thus formed were said to be functional in transforming D. pneumoniae to streptomycin resistance.
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References
Doty, P., Marmur, J., Eigner, J., and Schildkraut, C., Proc. U.S. Nat. Acad. Sci., 46, 461 (1960).
Marmur, J., and Lane, D., Proc. U.S. Nat. Acad. Sci., 46, 453 (1960).
Herriott, R., Proc. U.S. Nat. Acad. Sci., 47, 146 (1961).
Marmur, J., Lane, D., and Doty, P., unpublished results cited in Marmur, J., Rownd, R., and Schildkraut, C., Prog. Nucleic Acid Res., 1, 283 (1963).
Kent, J. L., Roger, M., and Hotchkiss, B. D., Proc. U.S. Nat. Acad. Sci., 50 717 (1963).
Bresler, S. E., Kreneva, R. A., Kushev, V. V., and Mosevitskii, M. I., J. Mol. Biol., 8, 79 (1964).
Ephrati-Elizur, E., Srinivasan, P., and Zamenhof, S., Proc. U.S. Nat. Acad Sci., 47, 56 (1961).
Nester, E. V., Schafer, M., and Lederberg, J., Genetics, 48, 529 (1963).
Marmur, J., J. Mol. Biol., 3, 208 (1961).
Schildkraut, C., Marmur, J., and Doty, P., J. Mol. Biol., 3, 595 (1961).
Spizizen, J., Fed. Proc., 18, 957 (1959).
Lacks, S., J. Mol. Biol., 5, 119 (1962).
Fox, M. S., and Allen, M. K., Proc. U.S. Nat. Acad. Sci., 52, 412 (1964).
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VESTRI, R., FELICETTI, L. & LOSTIA, O. Transformation by Hybrid DNA in Bacillus subtilis. Nature 209, 1154–1155 (1966). https://doi.org/10.1038/2091154b0
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DOI: https://doi.org/10.1038/2091154b0
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