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Starch-gel Electrophoresis of Anterior Pituitary Hormones

Nature volume 190, pages 629–630 (1961)Cite this article

Abstract

SEPARATION of pituitary hormones by zone electrophoresis on paper has not proved very successful, possibly due to complex formation between proteins1. Starch-gel zone electrophoresis introduced by Smithies2,3 separates proteins not only by their net charge but also by their molecular size. This procedure has superior resolving power to paper or agar-gel zone electrophoresis and has been successfully applied to the analysis of thyrotrophic hormone4–6. Starch-gel electrophoretic analyses of various standard preparations of anterior pituitary hormones are presented here together with some evidence of the distribution of hormonal activities.

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References

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Authors and Affiliations

  1. Ian Clunies Ross Animal Research Laboratory, Commonwealth Scientific and Industrial Research Organization, Division of Animal Physiology, Prospect, N.S.W.

    K. A. FERGUSON & A. L. C. WALLACE

Authors
  1. K. A. FERGUSON
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  2. A. L. C. WALLACE
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FERGUSON, K., WALLACE, A. Starch-gel Electrophoresis of Anterior Pituitary Hormones. Nature 190, 629–630 (1961). https://doi.org/10.1038/190629a0

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