Abstract
ABOUT ten years ago, it was shown1 that electron microscopic examination of ‘psuedo-replicas’ could reveal the ordered particle arrangement on the surface of a macromolecular crystal. The necessary preparations were made by metal-shadowing the dried crystals lying on a glass surface, coating with collodion, freeing this composite layer and dissolving the crystalline deposit from the replica thus produced. The molecular order on several kinds of crystal was seen in this way; but a broader application of the method was limited by factors such as the fragility of the collodion film and the need to work with proteins not denatured by vacuum desiccation and the evaporation of metal.
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Price, W. C., and Wyckoff, Ralph W. G., Nature, 157, 764 (1946).
Hall, C. E., J. Biol. Chem., 185, 45 (1951).
Bradley, D. E., Brit. J. App. Phys., 5, 65, 96 (1954).
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LABAW, L., WYCKOFF, R. Molecular Arrangement in Crystals of the Southern Bean Mosaic Virus Protein. Nature 176, 455 (1955). https://doi.org/10.1038/176455a0
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DOI: https://doi.org/10.1038/176455a0
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