Abstract
THE nucleic acids and their derivatives are fundamental constituents of biological systems ; but until recently workers have lacked precise micro methods for their study. Vischer and Chargaff1, and recently Hotchkiss2 and also Reichard3, have shown that partition chromatography4 may be used to separate and characterize certain derivatives. The methods employed by these workers to detect the spots on the chromatograms were, however, either limited in their scope or necessitated the analysis of a large number of fractions selected arbitrarily from the columns.
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References
Vischer, E., and Chargaff, E., J. Biol. Chem., 168, 781 (1947).
Hotchkiss, R. D., J. Biol. Chem. 175, 315 (1938).
Reichard, P., Nature, 162, 662 (1948).
Consden, R., Gordon, A. H., and Martin, A. J. P., Biochem. J., 38, 224 (1944).
Bowen, E. J., "Chemical Aspects of Light" (Clarendon Press, Oxford 1946).
Levene, P. A., and Bass, L. W., "Nucleic Acids" (The Chemical Catalog Co., Inc., New York, 1931).
Bredereck, H., Martini, A., and Richter, F., Ber. deutsch. chem. Ges., 74, 694 (1941).
Graff, S., and Maculla, A., J. Biol. Chem., 110, 71 (1935).
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MARKHAM, R., SMITH, J. Chromatography of Nucleic Acid Derivatives. Nature 163, 250–251 (1949). https://doi.org/10.1038/163250b0
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DOI: https://doi.org/10.1038/163250b0
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