Abstract
In order for cytotoxic T cells to initiate immune responses, peptides derived from internalized antigens must be presented to the cytotoxic T cells on major histocompatibility complex (MHC) class I molecules. Here we show that dendritic cells, the only antigen-presenting cells that initiate immune responses efficiently, have developed a unique membrane transport pathway linking the lumen of endocytic compartments and the cytosol. Endosome-to-cytosol transport is restricted to dendritic cells, specific to internalized antigens and selective for the size of the transported molecules. Thus, in dendritic cells, internalized antigens gain access to the cytosolic antigen-processing machinery and to the conventional MHC class I antigen-presentation pathway.
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Acknowledgements
We thank J.C. Antoine for providing macrophages, L. Cabanié for chromatography analysis, A. Trautman for advice on the T-cell Ca2+-activation experiments, M. Albert, P. Benaroch, and P. Pereira for helpful discussions and comments on the manuscript, W. Faigle for his help with Adobe Photoshop, J. M. Griffith for preparation of ultrathin cryosections, and R. Scriwaneck and D. Morineau for photographic work.
Correspondence and requests for materials should be addressed to S.A.
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Rodriguez, A., Regnault, A., Kleijmeer, M. et al. Selective transport of internalized antigens to the cytosol for MHC class I presentation in dendritic cells. Nat Cell Biol 1, 362–368 (1999). https://doi.org/10.1038/14058
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DOI: https://doi.org/10.1038/14058
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