Abstract
The transcription factor ER81 has been shown to be involved in ontogenesis and breast tumor formation. ER81 is activated by many signals through phosphorylation directly mediated by mitogen-activated protein kinases (MAPKs), but also by an unknown protein kinase(s). Here, mitogen- and stress-activated protein kinase 1 (MSK1), which itself is directly activated by distinct classes of MAPKs, is identified to regulate ER81 function. MSK1 expression enhances ER81-dependent transcription upon stimulation of especially the p38-MAPK pathway. Two serine residues in ER81 are phosphorylated by MSK1, and mutating these serine residues to alanines dramatically diminishes the ability of MSK1 to stimulate ER81. However, mutation of the MSK1 phosphorylation sites in ER81 does not completely abrogate the ability of MSK1 to activate ER81 function, suggesting that MSK1 may also target cofactors of ER81. Consistently, MSK1 interacts with two homologous coactivators of ER81, CBP and p300, and stimulates the transactivation domains of CBP. Thus, MSK1 may regulate ER81-dependent transcription via direct phosphorylation of ER81 as well as via stimulation of CBP/p300, which might be important for ER81's normal function and during mammary tumor formation.
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References
Ait-Si-Ali S, Ramirez S, Barre FX, Dkhissi F, Magnaghi-Jaulin L, Girault JA, Robin P, Knibiehler M, Pritchard LL, Ducommun B, Trouche D and Harel-Bellan A . (1998). Nature, 396, 184–186.
Arber S, Ladle DR, Lin JH, Frank E and Jessell TM . (2000). Cell, 101, 485–498.
Arthur JS and Cohen P . (2000). FEBS Lett., 482, 44–48.
Baert JL, Beaudoin C, Coutte L and de Launoit Y . (2002). J. Biol. Chem., 277, 1002–1012.
Barratt MJ, Hazzalin CA, Zhelev N and Mahadevan LC . (1994). EMBO J., 13, 4524–4535.
Ben-Levy R, Paterson HF, Marshall CJ and Yarden Y . (1994). EMBO J., 13, 3302–3311.
Berger SL . (2002). Curr. Opin. Genet. Dev., 12, 142–148.
Blough RI, Petrij F, Dauwerse JG, Milatovich-Cherry A, Weiss L, Saal HM and Rubinstein JH . (2000). Am. J. Med. Genet., 90, 29–34.
Bosc DG, Goueli BS and Janknecht R . (2001). Oncogene, 20, 6215–6224.
Bosc DG and Janknecht R . (2002). J. Cell. Biochem., 86, 174–183.
Bredemeier-Ernst I, Nordheim A and Janknecht R . (1997). FEBS Lett., 408, 47–51.
Brown TA, McKnight SL . (1992). Genes Dev., 6, 2502–2512.
Bruder JT, Heidecker G and Rapp UR . (1992). Genes Dev., 6, 545–556.
Chotteau-Lelievre A, Desbiens X, Pelczar H, Defossez PA and de Launoit Y . (1997). Oncogene, 15, 937–952.
Chotteau-Lelievre A, Dolle P, Peronne V, Coutte L, de Launoit Y and Desbiens X . (2001). Mech. Dev., 108, 191–195.
Chrivia JC, Kwok RP, Lamb N, Hagiwara M, Montminy MR and Goodman RH . (1993). Nature, 365, 855–859.
Cobb MH . (1999). Prog. Biophys. Mol. Biol., 71, 479–500.
Coutte L, Monte D, Imai K, Pouilly L, Dewitte F, Vidaud M, Adamski J, Baert JL and de Launoit Y . (1999). Oncogene, 18, 6278–6286.
Deak M, Clifton AD, Lucocq LM and Alessi DR . (1998). EMBO J., 17, 4426–4441.
Eckner R, Ewen ME, Newsome D, Gerdes M, DeCaprio JA, Lawrence JB and Livingston DM . (1994). Genes Dev., 8, 869–884.
Gayther SA, Batley SJ, Linger L, Bannister A, Thorpe K, Chin SF, Daigo Y, Russell P, Wilson A, Sowter HM, Delhanty JD, Ponder BA, Kouzarides T and Caldas C . (2000). Nature Genet., 24, 300–303.
Goodman RH and Smolik S . (2000). Genes Dev., 14, 1553–1577.
Goto H, Tomono Y, Ajiro K, Kosako H, Fujita M, Sakurai M, Okawa K, Iwamatsu A, Okigaki T, Takahashi T and Inagaki M . (1999). J. Biol. Chem., 274, 25543–25549.
Graves BJ and Petersen JM . (1998). Adv. Cancer Res., 75, 1–55.
Hung MC and Lau YK . (1999). Semin. Oncol., 26, 51–59.
Janknecht R . (1996). Mol. Cell. Biol., 16, 1550–1556.
Janknecht R . (2001). J. Biol. Chem., 276, 41856–41861.
Janknecht R . (2002). Histol. Histopathol., 17, 657–668.
Janknecht R, Ernst WH, Pingoud V and Nordheim A . (1993). EMBO J., 12, 5097–5104.
Janknecht R and Nordheim A . (1996). Biochem. Biophys. Res. Commun., 228, 831–837.
Jeon IS, Davis JN, Braun BS, Sublett JE, Roussel MF, Denny CT and Shapiro DN . (1995). Oncogene, 10, 1229–1234.
Kucera J, Cooney W, Que A, Szeder V, Stancz-Szeder H and Walro J . (2002). Dev. Dyn., 223, 389–401.
Kung AL, Rebel VI, Bronson RT, Ch'ng LE, Sieff CA, Livingston DM and Yao TP . (2000). Genes Dev., 14, 272–277.
Liu SL, Rand A, Kelm Jr RJ and Getz MJ . (2000). Oncogene, 19, 3352–3362.
Mahadevan LC, Willis AC and Barratt MJ . (1991). Cell, 65, 775–783.
Miller RW and Rubinstein JH . (1995). Am. J. Med. Genet., 56, 112–115.
Monte D, Coutte L, Baert JL, Angeli I, Stehelin D and de Launoit Y . (1995). Oncogene, 11, 771–779.
New L, Zhao M, Li Y, Bassett WW, Feng Y, Ludwig S, Padova FD, Gram H and Han J . (1999). J. Biol. Chem., 274, 1026–1032.
Nomura M, Kaji A, Ma WY, Zhong S, Liu G, Bowden GT, Miyamoto KI and Dong Z . (2001). J. Biol. Chem., 276, 25558–25567.
Papoutsopoulou S and Janknecht R . (2000). Mol. Cell. Biol., 20, 7300–7310.
Pearson KL, Hunter T and Janknecht R . (1999). Biochim. Biophys. Acta, 1489, 354–364.
Pierrat B, Correia JS, Mary JL, Tomas-Zuber M and Lesslauer W . (1998). J. Biol. Chem., 273, 29661–29671.
Rubin I and Yarden Y . (2001). Ann. Oncol., 12, S3–S8.
Sharrocks AD . (2001). Nature Rev. Mol. Cell Biol., 2, 827–837.
Shepherd TG, Kockeritz L, Szrajber MR, Muller WJ and Hassell JA . (2001). Curr. Biol., 11, 1739–1748.
Smits PH, de Wit L, van der Eb AJ and Zantema A . (1996). Oncogene, 12, 1529–1535.
Stein B, Yang MX, Young DB, Janknecht R, Hunter T, Murray BW and Barbosa MS . (1997). J. Biol. Chem., 272, 19509–19517.
Strelkov IS and Davie JR . (2002). Cancer Res., 62, 75–78.
Thomson S, Clayton AL, Hazzalin CA, Rose S, Barratt MJ and Mahadevan LC . (1999). EMBO J., 18, 4779–4793.
Tomas-Zuber M, Mary JL, Lamour F, Bur D and Lesslauer W . (2001). J. Biol. Chem., 276, 5892–5899.
van der Geer P and Hunter T . (1994). Electrophoresis, 15, 544–554.
Wiggin GR, Soloaga A, Foster JM, Murray-Tait V, Cohen P and Arthur JS . (2002). Mol. Cell. Biol., 22, 2871–2881.
Zhang Y, Liu G and Dong Z . (2001). J. Biol. Chem., 276, 42534–42542.
Zhong S, Jansen C, She QB, Goto H, Inagaki M, Bode AM, Ma WY and Dong Z . (2001). J. Biol. Chem., 276, 33213–33219.
Acknowledgements
I thank Maria Deak and Dario Alessi for providing MSK1 expression vectors, Yvan de Launoit for ERM and PEA3 encoding plasmids and Jianmin Wu for purification of some GST fusion proteins. This work was supported by a scholarship from the Sidney Kimmel Foundation for Cancer Research, the Mayo Foundation, and a grant from the National Cancer Institute (CA085257).
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Janknecht, R. Regulation of the ER81 transcription factor and its coactivators by mitogen- and stress-activated protein kinase 1 (MSK1). Oncogene 22, 746–755 (2003). https://doi.org/10.1038/sj.onc.1206185
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DOI: https://doi.org/10.1038/sj.onc.1206185
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