Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Paper
  • Published:

Physical interaction of the bHLH LYL1 protein and NF-κB1 p105

Oncogene volume 18, pages 995–1005 (1999)Cite this article

Abstract

The LYL1 gene was first identified upon the molecular characterization of the t(7;9)(q35;p13) translocation associated with some human T-cell acute leukemias (T-ALLs). In adult tissues, LYL1 expression is restricted to hematopoietic cells with the notable exclusion of the T cell lineage. LYL1 encodes a basic helix – loop – helix (bHLH) protein highly related to TAL-1, whose activation is also associated with a high proportion of human T-ALLs. A yeast two-hybrid system was used to identify proteins that specifically interact with LYL1 and might mediate its activities. We found that p105, the precursor of NF-κB1 p50, was the major LYL1-interacting protein in this system. The association between LYL1 and p105 was confirmed both in vitro and in vivo in mammalian cells. Biochemical studies indicated that the interaction was mediated by the bHLH motif of LYL1 and the ankyrin-like motifs of p105. Ectopic expression of LYL1 in a human T cell line caused a significant decrease in NF-κB-dependent transcription, associated with a reduced level of NF-κB1 proteins.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8

Similar content being viewed by others

References

  • Abbadie C, Kabrun N, Bouali F, Smardova J, Stehelin D, Banderbunder B and Enrietto PJ. . 1993 Cell 75: 899–912.

  • Aplan PD, Nakahara K, Orkin SH and Kirsch IR. . 1992 EMBO J. 11: 4073–4081.

  • Baer R. . 1993 Semin. Cancer Biol. 4: 341–347.

  • Baeuerle PA and Baltimore D. . 1996 Cell 87: 13–20.

  • Baichwal VR and Baeuerle PA. . 1997 Current Biol. 7: 94–96.

  • Bain G, Maandag ECR, Izon DJ, Amsen D, Kruisbeek AM, Weintraub BC, Krop I, Schlissel MS, Feeney AJ, Vanroon M, Vandervalk M, Teriele HPJ, Berns A and Murre C. . 1994 Cell 79: 885–892.

  • Bash RO, Hall S, Timmons CF, Crist WM, Amylon M, Smith RG and Baer R. . 1995 Blood 86: 666–676.

  • Beg AA and Baltimore D. . 1996 Science 274: 782–784.

  • Begley CG, Aplan PD, Denning SM, Haynes BF, Waldman TA and Kirsch IR. . 1989 Proc. Natl. Acad. Sci. USA 86: 10128–1032.

  • Bernard O, Guglielmi P, Jonveaux P, Cherif D, Gisselbrecht S, Mauchauffé M, Larsen CJ, Berger R and Mathieu-Mahul D. . 1990 Genes, Chromosomes, Cancer 1: 194–208.

  • Bernard O, Lecointe N, Jonveaux P, Souyri M, Mauchauffé M, Berger R, Larsen CJ and Mathieu-Mahul D. . 1991 Oncogene 6: 1477–1488.

  • Boothby MR, Mora AL, Scherer DC, Brocman JA and Ballard DW. . 1997 J. Exp. Med. 185: 1897–1907.

  • Brown L, Cheng JT, Chen Q, Siciliano MJ, Crist W, Buchanan G and Baer R. . 1990 EMBO J. 9: 3343–3351.

  • Carrasco D, Rizzo CA, Dorfman K and Bravo R. . 1996 EMBO J. 15: 3640–3650.

  • Chang CC, Zhang J, Lombardi L, Neri A and Dalla-Favera R. . 1995 Mol. Cell. Biol. 15: 5180–5187.

  • Chen Q, Cheng JT, Tsai LH, Schneider N, Buchanan G, Carroll A, Crist W, Ozanne B, Siciliano MJ and Baer R. . 1990 EMBO J. 9: 415–424.

  • Condorelli G, Tocci A, Botta R, Facchiano F, Testa U, Vitelli L, Valtieri M, Croce C and Peschle C. . 1997 Mol. Cell. Biol. 17: 2954–2969.

  • Condorelli GL, Facchiano F, Valtieri M, Proietti E, Vitelli L, Lulli V, Huebner K, Peschle C and Croce CM. . 1996 Cancer Research 56: 5113–5119.

  • Fields S and Song O. . 1989 Nature 340: 245–246.

  • Finco T and Baldwin AS. . 1995 Immunity 3: 263–272.

  • Fujimoto K, Yasudo H, Sato Y and Yamamoto K. . 1995 Gene 165: 183–189.

  • Gilmore TD, Koedood M, Piffat KA and White DW. . 1996 Oncogene 13: 1367–1378.

  • Goldfarb AN, Goueli S, Michelson D and Greenberg JM. . 1992 Blood 80: 2658–2666.

  • Green AR, DeLuca E and Begley GC. . 1991a EMBO J. 10: 4153–4158.

  • Green AR, Salvaris RE and Begley GC. . 1991b Oncogene 6: 475–479.

  • Grilli M, Pizzi M, Memo M and Spano PF. . 1996 Science 274: 1383–1385.

  • Grimm S, Bauer MKA, Baeuerle PA and Schulse-Olthoff K. . 1996 J. Cell. Biol. 134: 13–23.

  • Hsu HL, Cheng J, Chen Q and Baer R. . 1991 Mol. Cell. Biol. 11: 3037–3042.

  • Hsu HL, Huang L, Tsou Tsan J, Funk W, Wright WE, Hu JS, Kingston RE and Baer R. . 1994a Mol. Cell. Biol. 14: 1256–1265.

  • Hsu H, Wadman I and Baer R. . 1994b Proc. Natl. Acad. Sci. USA 91: 3181–3185.

  • Hsu HL, Wadman I, Tsou Tsan J and Baer R. . 1994c Proc. Natl. Acad. Sci. USA 91: 5947–5951.

  • Kallianpur A, Jordan JE and Brandt SJ. . 1994 Blood 83: 1200–1208.

  • Kang SM, Tran AC, Grilli M and Lenardo MJ. . 1992 Science 256: 1452–1456.

  • Kelliher MA, Seldin DC and Leder P. . 1996 EMBO J. 15: 5160–5166.

  • Klein ES, Simmons DM, Swanson LW and Rosenfeld MG. . 1993 Genes Dev. 7: 55–71.

  • Leroy-Viard K, Vinit MA, Lecointe N, Jouault H, Hibner U, Roméo PH and Mathieu-Mahul D. . 1995 EMBO J. 14: 2341–3249.

  • Liu Z, Hsu H, Goeddel DV and Karin M. . 1996 Cell 87: 565–576.

  • MacKichan ML, Logeat F and Israel A. . 1996 J. Biol. Chem. 271: 6084–6091.

  • Mahajan MA, Park ST and Sun XH. . 1996 Oncogene 12: 2343–2350.

  • Mellentin JD, Smith SD and Cleary ML. . 1989 Cell 58: 77–83.

  • Migliazza A, Lombardi L, Rocchi M, Trecca D, Chang CC, Antonacci R, Fracchiolla NS, Ciana P, Maiolo AT and Neri A. . 1994 Blood 84: 3850–3860.

  • Miyamoto A, Cui XM, Naumovski L and Cleary ML. . 1996 Mol. Cell. Biol. 16: 2394–2401.

  • Mouthon MA, Bernard O, Mitjavila MT, H. RP, Vainchenker W and Mathieu-Mahul D. . 1993 Blood 81: 647–655.

  • Munoz E, Courtois G, Veschambre P, Jalinot P and Israel A. . 1994 J. Virol. 68: 8035–8044.

  • Murre C, Caw M, Schonleber P, Vaessin H, Caudy M, Jan LY, Jan YN, Cabrera CV, Buskin JN, Hauschka SD, Lassar AB, Weintraub H and Baltimore D. . 1989a Cell 58: 777–783.

  • Murre C, Schonleber McCaw P and Baltimore D. . 1989b Cell 56: 777–783.

  • Ono Y, Fukuhara N and Yoshie O. . 1997 J. Biol. Chem. 272: 4576–4581.

  • Palombella VJ, Rando OJ, Goldberg AL and Maniatis T. . 1994 Cell 78: 773–785.

  • Porcher C, Swat W, Rockwell K, Fujiwara Y, Alt FW and Orkin SH. . 1996 Cell 86: 47–57.

  • Pulford K, Lecointe N, Leroy-Viard K, Jones M, Mathieu-Mahul D and Mason DY. . 1995 Blood 85: 675–684.

  • Robb L, Elwood NJ, Elefanty AG, Kontgen F, Li RL, Barnett LD and Begley CG. . 1996 EMBO J. 15: 4123–4129.

  • Robb L, Lyons I, Li RL, Hartley L, Kontgen F, Harvey RP, Metcalf D and Begley CG. . 1995 Proc Natl. Acad. Sci. USA 92: 7075–7079.

  • Rousset R, Desbois C, Bantignies F and Jalinot P. . 1996 Nature 381: 328–331.

  • Shivdasani RA, Mayer EL and Orkin SH. . 1995 Nature 373: 432–434.

  • Takebe Y, Seiki M, Fujisawa JI, Hoy P, Yokota K, Arai KI, Yoshida M and Arai N. . 1988 Mol. Cell. Biol. 8: 466–472.

  • Ten RM, Paya CV, Israel N, LeBail O, Mattei MJ, Virelizier JL, Kourilsky P and Israel A. . 1992 EMBO J. 11: 195–203.

  • Valge-Archer VE, Osada H, Warren AJ, Forster A, Li J, Baer R and Rabbitts TH. . 1994 Proc. Natl. Acad. Sci. USA 91: 8617–8621.

  • Van Antwerp DJ, Martin SJ, Kafri T, Green DR and Verma IM. . 1996 Science 274: 787–789.

  • Visvader J, Begley GC and Adams J. . 1991 Oncogene 6: 187–194.

  • Wadman I, Li JX, Bash RO, Forster A, Osada H, Rabbitts TH and Baer R. . 1994 EMBO J. 13: 4831–4839.

  • Wadman I, Osada H, Grutz G, Agulnick A, Westphal H, Forster A and Rabbitts T. . 1997 EMBO J. 16: 3145–3157.

  • Xia Y, Hwang LY, Cobb MH and Baer R. . 1994 Oncogene 9: 1437–1446.

  • Xia YLB, Ying-Chuan Yang C, Tsou Tsan J, Siciliano MJ, Espinosa III R, Le Beau M and Baer R. . 1991 Proc. Natl. Acad. Sci. USA 88: 11416–11420.

Download references

Acknowledgements

We are grateful to Urszula Hibner, Naomi Taylor and Robert Hipskind for helpful discussion and critical comments on the manuscript. We greatly appreciate Cécile Lambert for her excellent technical assistance. We are greatly indebted to Alain Israel (Institut Pasteur, Paris, France) for providing us with the (Igκ)3-conaluc plasmid and the anti p50/p105 antibody, to Michael Clearly and Alison Miyamoto (Stanford University, School of Medecine, USA) for the anti LYL1 antibody, to Jacques Camonis (Institut Curie, Paris, France) for the Jurkat cDNA library, to Marc-Henri Stern (Hôpital Saint Louis, Paris, France) for the pGAD-I-κBα plasmid, Brigitte Royer-Pokora (Heidelberg, Germany) for the Lmo2/TTG2 cDNA and JM Blanchard (Montpellier, France) for the anti GAPDH antibody. This work was supported in part by grants from the `Association pour la Recherche sur le Cancer' (ARC), the `Ligue Nationale contre le Cancer' and the `Fondation contre la Leucémie'. RF is supported by a fellowship from the `Association pour la Recherche sur le Cancer' (ARC).

Author information

Authors and Affiliations

  1. Institut de Génétique Moléculaire, UMR 5535, 1919 Route de Mende, Montpellier, 34293, F, France

    Richard Ferrier, Romain Nougarede, Sandrine Doucet & Danièle Mathieu-Mahul

  2. INSERM U119, Institut Paoli-Calmettes, 27 Boulevard Leï Roure, Marseille, 13009, F, France

    Brigitte Kahn-Perles & Jean Imbert

Authors
  1. Richard Ferrier
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Romain Nougarede
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sandrine Doucet
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Brigitte Kahn-Perles
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jean Imbert
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Danièle Mathieu-Mahul
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ferrier, R., Nougarede, R., Doucet, S. et al. Physical interaction of the bHLH LYL1 protein and NF-κB1 p105. Oncogene 18, 995–1005 (1999). https://doi.org/10.1038/sj.onc.1202374

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1202374

Keywords

This article is cited by

Search

Advanced search

Quick links