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A mouse model of intracerebral hemorrhage using autologous blood infusion

Nature Protocols volume 3, pages 122–128 (2008)Cite this article

Abstract

The development of controllable and reproducible animal models of intracerebral hemorrhage (ICH) is essential for the systematic study of the pathophysiology and treatment of hemorrhagic stroke. In recent years, we have used a modified version of a murine ICH model to inject blood into mouse basal ganglia. According to our protocol, autologous blood is stereotactically infused in two stages into the right striatum to mimic the natural events of hemorrhagic stroke. Following ICH induction, animals demonstrate reproducible hematomas, brain edema formation and marked neurological deficits. Our technique has proven to be a reliable and reproducible means of creating ICH in mice in a number of acute and chronic studies. We believe that our model will serve as an ideal paradigm for investigating the complex pathophysiology of hemorrhagic stroke. The protocol for establishing this model takes about 2 h.

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Figure 1: A picture representing a setup of the stereotactic apparatus and other devices used in the ICH model.
Figure 2: A mouse mounted in the stereotactic frame.
Figure 3: Representative landmarks of mouse skull.
Figure 4: Representative histological photomicrographs of low- (a) and high- (b) magnification, hematoxylin and eosin (H&E)-stained brain section obtained 3 d after injection of 30 μl of autologous blood into the right striatum of C57BL/6J mouse.
Figure 5: Typical results.

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Acknowledgements

Dr. M.A.R. was supported by The Kosciuszko Foundation Postdoctoral Research Fellowship.

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Authors and Affiliations

  1. Department of Neurological Surgery, Columbia University, 710 West 168th Street, New York, 10032, New York, USA

    Michal A Rynkowski, Grace H Kim, Ricardo J Komotar, Marc L Otten, Andrew F Ducruet, Brad E Zacharia, Christopher P Kellner, David K Hahn, Maxwell B Merkow, Matthew C Garrett, Robert M Starke, Byung-Moon Cho, Sergei A Sosunov & E Sander Connolly

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Correspondence to Ricardo J Komotar.

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Rynkowski, M., Kim, G., Komotar, R. et al. A mouse model of intracerebral hemorrhage using autologous blood infusion. Nat Protoc 3, 122–128 (2008). https://doi.org/10.1038/nprot.2007.513

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