Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Association of CD2 and CD45 on human T lymphocytes

Abstract

AT least two membrane receptors have been defined through which human T lymphocytes can be induced to proliferate and differentiate, namely the CD3-Ti antigen receptor complex1 and the CD2 molecule2. Monoclonal antibodies directed at either CD2 or CD3 induce intracellular second messenger production and subsequent protein phosphorylation3–5. On most human non-B lymphocytes, CD3-Ti and CD2 are coexpressed and seem to be functionally interrelated6. But there are minor subpopulations in which these receptor systems can transduce signals despite a mutually exclusive expression7,8, indicating that CD3-Ti and CD2 can act independently of each other. This view is supported by the finding that most monoclonal antibodies directed at the CD45 molecules9,10 are strongly co-mitogenic with CD2 but not CD3 monoclonal antibodies (refs 11,12). As the intracytoplasmic domains of CD45 have tyrosine phosphatase activity13 these functional effects11,12 could be explained by a physical association between CD2 and CD45. Using chemical crosslinking techniques, we now show that CD45 is linked to CD2 on the surface of human T lymphocytes.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Meuer, S. C. et al. J. exp. Med. 157, 705–720 (1983).

    Article  CAS  Google Scholar 

  2. Meuer, S. C. et al. Cell 36, 897–906 (1984).

    Article  CAS  Google Scholar 

  3. Breitmeyer, J. B., Daley, J. F., Levine, H. B. & Schlossman, S. F. J. immun. 139, 2899–2905 (1987).

    CAS  PubMed  Google Scholar 

  4. Cantrell, D. A., Verbi, W., Davies, A., Parker, P. & Crumpton, M. J. Eur. J. Immun. 18, 1391–1396 (1988).

    Article  CAS  Google Scholar 

  5. Meuer, S. C. et al. J. exp. Med. 158, 988–993 (1983).

    Article  CAS  Google Scholar 

  6. Alcover, A. et al. EMBO J. 7, 1973–1977 (1988).

    Article  CAS  Google Scholar 

  7. Silicano, R. F., Pratt, J. C., Schmidt, R. E., Ritz, J. & Reinherz, E. L. Nature 317, 428–430 (1985).

    Article  ADS  Google Scholar 

  8. Faure, F. et al. J. Immun. 140, 2128–2132 (1988).

    CAS  PubMed  Google Scholar 

  9. Morimoto, C., Letvin, N. L., Distaso, J. A., Aldrich, W. R. & Schlossman, S. F. J. Immunol. 134, 1508–1515 (1985).

    CAS  PubMed  Google Scholar 

  10. Streuli, M., Morimoto, C., Schrieberg, M., Schlossman, S. F. & Saito, H. J. Immun. 141, 3910–3914 (1988).

    CAS  PubMed  Google Scholar 

  11. Schraven, B., Roux, M., Hutmacher, B. & Meuer, S. C. Eur. J. Immun. 19, 397–403 (1989).

    Article  CAS  Google Scholar 

  12. Schraven, B., Roux, M., Hutmacher, B. & Meuer, S. C. Leucocyte Typing IV, 640–643 (Oxford University Press, 1989).

    Google Scholar 

  13. Tonks, N. K., Charbonneau, H. Diltz, C. D., fisher, E. H. & Walsh, K. A. Biochemistry 27, 8695–8701 (1989).

    Article  Google Scholar 

  14. Bernard, A., Gelin, C., Rayal, B., Pham, D., Gosse, C. & Boumsell, L. J. exp. Med. 155, 1317–1333 (1982).

    Article  CAS  Google Scholar 

  15. Holter, W., Fisher, G. F., Majdhic, O., Stockinger, H. & Knapp, W. J. exp. Med. 163, 654 (1986).

    Article  CAS  Google Scholar 

  16. Young, S. Y., Chouaib, S. & Dupont, B. J. Immun. 137, 1097–1100 (1986).

    Google Scholar 

  17. Thompson, C. B. et al. Proc. natn. Acad. Sci. U.S.A. 86, 1333–1337 (1989).

    Article  ADS  CAS  Google Scholar 

  18. Tonks, N. K., Diltz, C. D. & Fisher, E. H. J. biol. Chem. 263, 6731–6737 (1988).

    CAS  PubMed  Google Scholar 

  19. Cleveland, D. W., Fisher, S. G., Kirschner, M. W. & Laemmli, U. K. J. biol. Chem. 252, 1102–1106 (1977).

    CAS  Google Scholar 

  20. Ledbetter, J. A., Tanks, N. K., Fisher, E. H. & Clark, E. A. Proc. natn. Acad. Sci. U.S.A. 85, 8628–8632 (1988).

    Article  ADS  CAS  Google Scholar 

  21. Charbonneau, H., Tonks, N. K., Walsh, K. A. & Fisher, E. H. Proc. natn. Acad. Sci. U.S.A. 85, 7182–7186 (1988).

    Article  ADS  CAS  Google Scholar 

  22. Turco, M. C., de Felice, M., Corbo, L., Giarrusso, P. C., Yang, S. Y., Ferronet, S. & Venuta, S. J. Immun. 141, 2275–2281 (1988).

    CAS  PubMed  Google Scholar 

  23. Eye, P. L., Prowse, S. J. & Jenkin, C. R. Immunochemistry 15, 429–440 (1978).

    Article  Google Scholar 

  24. Altevogt, P. et al. Int. Immunol. (in the press).

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schraven, B., Samstag, Y., Altevogt, P. et al. Association of CD2 and CD45 on human T lymphocytes. Nature 345, 71–74 (1990). https://doi.org/10.1038/345071a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/345071a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing