Abstract
DEGRADATIVE work on the phycobilin prosthetic groups of the algal biliproteins (phycoerythrins arid phycocyanins) has been hampered by the difficulties involved in splitting off these prosthetic groups in native form and isolating them in sufficiently high yields for classical degradation and analysis. These difficulties have now been avoided by using the chromic acid micro-degradation technique recently refined for the study of aplysioviolin1, the defence pigment of the sea hare. This technique may be used with as little as 100 µg of bilin and is not affected by the presence of protein (Rüdiger and Klose, W., unpublished), thus enabling direct degradation of the phycobilins while still attached to the proteins. This development, together with the improved resolution of the Chromatographic techniques used, has led to the identification of side chain groupings not previously detected in these prosthetic groups.
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RÜDIGER, W., CARRA, P. & HEOCHA, C. Structure of Phycoerythrobilin and Phycocyanobilin. Nature 215, 1477–1478 (1967). https://doi.org/10.1038/2151477a0
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DOI: https://doi.org/10.1038/2151477a0
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