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Preparation of Retinene in Vitro

Abstract

RETINENE, obtained by Wald from dark-adapted retinas, is defined as the chromogen responsible for a maximum at 664 mµ in the absorption spectrum of the blue solution obtained by mixing retinal extracts with antimony trichloride in chloroform. Such extracts show (in chloroform) an ultra-violet absorption band with λmax.385 mµ. It has recently been suggested1 that the chromogen is vitamin A aldehyde, and that it can be obtained by direct oxidation of vitamin A alcohol.

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References

  1. Morton, NATURE, 153, 69 (1944).

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  2. Hunter and Hawkins, NATURE, 153, 194 (1944).

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MORTON, R., GOODWIN, T. Preparation of Retinene in Vitro. Nature 153, 405–406 (1944). https://doi.org/10.1038/153405a0

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