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The Induction of Chromosomal Aberrations by Tetra Antibiotic in Bone Marrow Cells of Rats in Vivo

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Abstract

The aim of this study was to investigate the in vivo effects of tetra (tetralet) antibiotic on chromosomal aberrations (CA) in bone marrow cells of rats (Rattus norvegicus var. albinos). The tetra antibiotic significantly increased the percentage of abnormal cells and the chromosomal aberrations per cell (CA/cell) in bone marrow cells of rats at concentrations of 100 and 200 mg/kg body weight for 12 and 24 h treatment periods for each. In addition, the percentage of abnormal cells and the CA/cell increased dose-dependently for 12 h treatment period; in contrast, mitotic index was decreased when compared with negative control and solvent controls for 12-h treatment period. However, mitotic index increased depending on tetra antibiotic dose for 24-h treatment period.

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REFERENCES

  1. Agüloglu, S. and Ortakaya, C., Erytromycin’in Insan Kromozomlari Üzerine In VitroEtkileri, XI. Ulusal Biyoloji Kongresi, Elazig. Genel Biyoloji, 1992, pp. 33-43.

  2. Martelli, A., Mattioli, F., Pastorino, G., et al., Genotoxicity Testing of Chloramphenicol in Rodent and Human Cells, Mutation Res., 1993, vol. 260, pp. 65-72.

    Google Scholar 

  3. Neu, R., Apillaga, M.J., and Gardner, L.I., Effect of Antibiotics on Chromosomes of Cultured Human Lymphoctes, Nature, 1965, vol. 205, pp. 171-172.

    Google Scholar 

  4. Sbrana, I., Caretto, V., Rainaldi, G., and Loprieno, N., Induction of Chromosomal Aberrations and SCE by Chloramphenicol, Mutation Res., 1991, vol. 248, pp. 145-153.

    Google Scholar 

  5. Zaninovic, T., Papes, D., and Faranekic, J., Cyclohexemide Genotoxicity in Vitro and in Vivo Test Systems, Mutation Res., 1991, vol. 263, pp. 203-210.

    Google Scholar 

  6. Wilmer,J.L. and Bloom, S.E., A Cytogenetic Approach for Detecting the Selective Toxicity of Arugs in Avian Embrionic B and T Lymphocytes, Mutation Res., 1991, vol. 253, pp. 161-172.

    Google Scholar 

  7. Agüloglu, S. and Ortakaya, C., Gentamisin’in Sitogenetik Etkileri, XII. Ulusal Biyoloji Kongresi, 1994, pp. 208-216.

  8. Marchetti, C., Tiveron, C., Bassani, B., and Pacchierotti, F., Griseofulvin-Induced Aneuploidy and Meiotic Delay in Female Mouse Germ Cells. II. Cytogenetic Analysis of One-Cell Zygotes, Mutation Res., 1992, vol. 266, pp. 151-162.

    Google Scholar 

  9. Mavournin, K.H., Blakey, V., Climino, M.C., et al., The in Vivo Micronuclei Assay in Mammalian Bone Marrow and Peripheral Blood. A report of the U. S. environmental protection agency gene-tox. Program, Mutation Res., 1990, vol. 239, pp. 29-80.

    Google Scholar 

  10. Tiveron, C., Marchetti, F., Bassani, B., and Pacchierotti, F., Grisefulvin-Induced Aneuploidy and Meiotic Delay in Female Mouse Germ Cells. I. Cytogenetic Analysis of Metaphase II Oocytes, Mutation Res., 1992, vol. 266, pp. 143-150.

    Google Scholar 

  11. Pasaraki, K. and Demopoulus, N.A., Induction of Chromosome Damage and Sister-Chromatid Exchange in Human Lymphocytes by the Anti-Tumour Antibiotic Neocarzinostatin, Mutation Res., 1988, vol. 204, pp. 669-673.

    Google Scholar 

  12. Rao, V. V. N. G., Gupta, E. V. V., and Thomas, I. M., Chromosomal Aberrations in Tuberculosis Patients before and after Treatment with Short Term Chemotherapy, Mutat. Res., 1991, vol. 259, pp. 13-19.

    Google Scholar 

  13. Bilge, E., Gözükirmizi, N., and Topaktas, M., Bazi mutajenlere Maruz Birakilmis Zafer-160 Arpasmda Sitogenetik Incelemeler, TÜBITAK VI. Bilim Kongresi, Matematik, Fiziki ve Biyolojik Bilimler Arastirma Grubu Tebligleri Biyoloji Seksiyonu, 1977, pp. 143-150.

  14. Kumar, U. and Sinha, S.S., Genotoxic effects of Two Pesticides (Rogor and Bavistin) and an Antibiotic (Streptomycin) in Meiotic Cells of Grasspea (Lathyrus sativusL.), Cytologia, 1991, vol. 56, pp. 209-214.

    Google Scholar 

  15. Badr, A., Cytogenetic Activities of 3 Sulphonamides, Mutat. Res., 1982, vol. 104, pp. 95-100.

    Google Scholar 

  16. Ila, H. B. and Topaktas, M., In vivo Genotoxic Effects of Spiramycin in Rat Bone Marrow Cells, Cytologia, 1999, vol. 64, pp. 277-283.

    Google Scholar 

  17. Madigan, M. T., Martinko, M., and Parker, S., Brock Biology of Microbiology, 8th ed., New York: Press Prentice Hall International Inc., 1997.

    Google Scholar 

  18. Von Sengbusch, P., Molekular-und Zellbiologie, New York: Berlin; Heidelberg: Springer-Verlag, 1979.

    Google Scholar 

  19. Van-den Bogert, C., Dontje, B.H., Hotrop, M., et al., Arrest of the Proliferation of Renal and Prostate Carcinomas of Human Orijin by Inhibition of Mitochondrial Protein Synthesis, Cancer, 1986, vol. 46, no. 7, pp. 3283-3289.

    Google Scholar 

  20. Kallio, M. and Lahdetie, J., Effects of DNA Topoisomerase in Inhibitor Merbarone in Male Mouse Meiotic Divisions in Vivo: cell cycle arrest and induction of aneuploidy, Environ. Mol. Mutagen, 1997, vol. 29, no. 1, pp. 16-27.

    Google Scholar 

  21. Knippers, R., Schäfer, K.P., and Fanning, E., Molekulare Genetik, 4th ed., New York, 1995 Völlig Neubear. U. Verb. Aufl. Georg Thieme Verlag, Stuttgart.

  22. Sylianco, C. Y. L. B., Balanco, F. R. B., and Lim, C. M., Mutagenicity, Clastogenicity and Antimutagenicity of Akapulko Decoctions and Lotion, Naturel and Applied Science Bulletin Philippines, 1987, vol. 39, no. 1, pp. 49-57.

    Google Scholar 

  23. Villasenor, I. M., Isolation and Structure Elucidation of Anti-Genotoxic Constituents of Cucurbita Maxima Duchesne. U. P. ((University-of-the-Philippines)-Research-Digest Philippines), 1995, vols. 2 (2)-3 (1), p. 54.

  24. Hartman, A., Wess, D., and Witte, L, Enhanced Cyto-And Genotoxicity of Tetracycline in Wilson Disease Fibroblast, Mutat. Res., 1995, vol. 348, pp. 7-12.

    Google Scholar 

  25. Sbrana, I., Caretto, V., Rainaldi, G., and Loprieno, N., Induction of Chromosomal Aberrations and SCE by Chloramphenicol, Mutat. Res., 1991, vol. 248, pp. 145-153.

  26. Duivenvoorden, W.C., Hirte, H.W., and Singh, G., Use of Tetracycline as an Inhibitor of Matrix Metalloproteinase Activity Secreted by Human Bone-Metastasizing Cancer Cells, Invasion Metastasis, 1997, vol. 17, no. 6, pp. 312-322.

    Google Scholar 

  27. Gu, Y., Lee, H.M., Roemer, E.J., et al., Inhibition of Tumor Cells Invasiveness by Chemically Modified Tetracyclines, Curr. Med. Chem., 2001, vol. 8, no. 3, pp. 261-270.

    Google Scholar 

  28. Chevassut, T. and Lim, B., Insight into the Role of DNA Methylation in Murine Embriyonic Stem Cells Using a Modified Tetracycline-Inducible Gene Expression System, Oncol. Res., 2003, vol. 13 (6-10), pp. 373-380.

    Google Scholar 

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  1. Department of Biology, Çukurova University, Institute of Naturel and Applied Sciences, Adana, 01330, Turkey

    T. Çakmak, M. Topakta & A. Kayraldiz

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  1. T. Çakmak
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  2. M. Topakta
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  3. A. Kayraldiz
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Çakmak, T., Topakta, M. & Kayraldiz, A. The Induction of Chromosomal Aberrations by Tetra Antibiotic in Bone Marrow Cells of Rats in Vivo. Russian Journal of Genetics 40, 867–870 (2004). https://doi.org/10.1023/B:RUGE.0000039720.98007.a5

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