Abstract
Recombinant tumstatin was expressed in stably transformed Drosophila melanogaster S2 cells and secreted into the medium with a molecular size of 29 kDa. Recombinant endostatin was also purified to homogeneity using a simple one-step Ni2+ affinity fractionation. Purified recombinant tumstatin inhibited endothelial cell proliferation in a dose-dependent manner. The concentration at half-maximum inhibition for recombinant tumstatin was approx. 0.7 μg ml−1. A maximum production of 4.6 μg recombinant tumstatin (107 cells)−1 was obtained in a T-flask culture of S2 cells, 7 d after induction with 0.5 mM CuSO4.
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Jeon, H.K., Chang, K.H., Kim, K.I. et al. Functional expression of recombinant tumstatin in stably transformed Drosophila melanogaster S2 cells. Biotechnology Letters 25, 185–189 (2003). https://doi.org/10.1023/A:1022330429508
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DOI: https://doi.org/10.1023/A:1022330429508