Abstract
Bio-imprinting has been introduced as a technique of interfacial activation of lipase for anhydrous reaction applications. In this study, air–water (bubble) interfaces were compared to amphiphile and substrate interfaces in microbial lipase bio-imprinting. Results indicated that the bubble interface is equally effective on lipase interesterification activity and produces a 4–4.5-fold increase compared with the enzymes as supplied. Interesterification activity can be explained in terms of effects upon the accessibility of the lipase active site. This technique provides an easier, cheaper and product-friendly way of lipase bio-imprinting.
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References
Akoh, C.C. 1995 Structured lipids-enzymatic approach. INFORM 6, 1055–1064.
Gonzales-Navarro, H. & Braco, L. 1997 Improving lipase activity in solvent-free media by interfacial activation-based molecular bioimprinting. Journal of Molecular Catalysis B: Enzymatic 3, 111–119.
Gonzales-Navarro, H. & Braco, L. 1998 Lipase-enhanced activity in flavour ester reactions by trapping enzyme conformers in the presence of interfaces. Biotechnology and Bioengineering 59, 122–127.
Gupta, M.N., Batra, R., Tyagi, R. & Sharma, A. 1997 Polarity index: the guiding solvent parameter for enzyme stability in aqueous–organic cosolvent mixtures. Biotechnology Progress 13, 284–288.
Klibanov, A.M. & Dabulis, K. 1993 Dramatic enhancement of enzymatic activity in organic solvents by lyoprotectants. Biotechnology Bioengineering 41, 566–571.
Klibanov, A.M. & Russell, A.J. 1988 Inhibitor-induced enzyme activation in organic solvents. Journal of Biological Chemistry 263, 11,624–11,626.
Laane, C., Boeren, S., Vos, K. & Veeger, C. 1987 Rules for optimization of biocatalysis in organic solvents. Biotechnology Bioengineering 30, 81–87.
Mingarro, I., Braco, L. & Abad, C. 1995 Interfacial activation-based molecular bioimprinting of lipolytic enzymes. Proceedings of the National Academy of Sciences of the USA 92, 3308–3312.
Mosbach, K., Stahl, M., Jeppson-Wistrand, U. & Mansson, M.-O. 1991 Induced stereoselectivity and substrate selectivity of bioimprinted α-chymotrypsin in anhydrous organic media. Journal of the American Chemical Society 113, 9366–9368.
Nakajima, M., Maruyama, T., Ichikawa, S., Nabetani, H., Furusaki, S. & Seki, M. 2000 Oil–water interfacial activation of lipase for interesterification of triglyceride and fatty acid. Journal of the American Oil Chemists Society 77, 1121–1126.
Peters, G.H., van Aalten, D.M.F., Edholm, O., Toxvaerd, S. & Bywater, R. 1996 Dynamics of proteins in different solvent systems: analysis of essential motion in lipases. Biophysical Journal 71, 2245–2255.
Sarda, L. & Desnuelle, P. 1958 Action de la lipase pancreatique sur les ester en emulsion. Biochimica et Biophysica Acta 30, 513–521.
Weete, J.D. 1998 Microbial lipases. In Food Lipids: Chemistry, Nutrition, and Biotechnology, eds. Akoh, C.C. & Min, D.B. New York: Marcel Dekker, Inc. ISBN 0-82479985-2.
Wul., G., Gross, T. & Schönfeld, R. 1997 Enzyme models based on molecularly imprinted polymers with strong esterase activity. Angewandte Chemie International Edition in English 36, 1962–1964.
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Yilmaz, E. Bio-imprinting of microbial lipase at air–water interface. World Journal of Microbiology and Biotechnology 18, 141–145 (2002). https://doi.org/10.1023/A:1014401027903
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DOI: https://doi.org/10.1023/A:1014401027903