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A novel second isoenzyme of the human UDP-N-acetylglucosamine:α1,3-D-mannoside β1,4-N-acetylglucosaminyltransferase family: cDNA cloning, expression, and chromosomal assignmen

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Abstract

We isolated a novel cDNA encoding a second isoenzyme of UDP-N-acetylglucosamine:α1,3-D-mannoside β1,4-N-acetylglucosaminyltransferase (GnT-IV; EC 2.4.1.145). The nucleotide and deduced amino acid sequences of the cDNA were homologous to those of the previously cloned human GnT-IV cDNA (63% and 62% identity, respectively). The new cDNA is also confirmed to express GnT-IV activity, suggesting that two isoenzymes of human GnT-IV exist. Although genomic Southern analysis suggested that both genes exist in many mammalian species and the chicken, northern analysis revealed that both genes are expressed in different ways in human tissues. This is the first report concerning the gene family of an N-acetylglucosaminyltransferase in mammals.

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References

  1. Allen SD, Tsai D, Schachter, H (1984) J Biol Chem 259: 6984–90.

    Google Scholar 

  2. Gleeson, PA, and Schachter, H (1983) J Biol Chem 258: 6162–73.

    Google Scholar 

  3. Yamashita, K, Tachibana, Y, Ohkura, T, and Kobata, A (1985) J Biol Chem 260: 3963–9.

    Google Scholar 

  4. Koenderman AH, Wijermans PW, van den Eijnden DH (1987) FEBS Lett 222: 42–6.

    Google Scholar 

  5. Nishikawa A, Gu J, Fujii S, Taniguchi N (1990) Biochim Biophys Acta 1035: 313–8.

    Google Scholar 

  6. Nakao H, Nishikawa A, Karasuno T, Nishiura T, Iida M, Kanayama Y, Yonezawa T, Tarui S, Taniguchi N (1990) Biochem Biophys Res Commun 172: 1260–6.

    Google Scholar 

  7. Ogier-Denis E, Bauvy C, Moutsita R, Aubery M, Codogno P (1990) Biochim Biophys Acta 1054: 149–53.

    Google Scholar 

  8. Oguri S, Minowa MT, Ihara Y, Taniguchi N, Ikenaga H, Takeuchi M (1997) J Biol Chem 272: 22721–7.

    Google Scholar 

  9. Schachter H, Brockhausen I, Hull E (1989) Methods Enzymol 179: 351–97.

    Google Scholar 

  10. Yoshima H, Matsumoto A, Mizuochi T, Kawasaki T, Kobata A (1981) J Biol Chem 256: 8476–84.

    Google Scholar 

  11. Yamashita K, Kamerling JP, Kobata A (1982) J Biol Chem 257: 12809–14.

    Google Scholar 

  12. Takeuchi M, Takasaki S, Miyazaki H, Kato T, Hoshi S, Kochibe N, Kobata A (1988) J Biol Chem 263: 3657–63.

    Google Scholar 

  13. Yamashita K, Totani K, Iwaki Y, Takamisawa I, Tateishi N, Higashi T, Sakamoto Y, Kobata A (1989) J Biochem (Tokyo) 105: 728–35.

    Google Scholar 

  14. Sato T, Furukawa K, Autero M, Gahmberg CG, Kobata A (1993) Biochemistry 32: 12694–704.

    Google Scholar 

  15. Treuheit MJ, Costello CE, Kirley TL (1993) J Biol Chem 268: 13914–9.

    Google Scholar 

  16. Treumann A, Lifely MR, Schneider P, Ferguson MA (1995) J Biol Chem 270: 6088–99.

    Google Scholar 

  17. Bloom JW, Madanat MS, Ray MK (1996) Biochemistry 35: 1856–64.

    Google Scholar 

  18. Yamashita K, Tachibana Y, Kobata A (1978) J Biol Chem 253: 3862–9.

    Google Scholar 

  19. Perng GS, Shoreibah M, Margitich I, Pierce M, Fregien N (1994) Glycobiology 4: 867–71.

    Google Scholar 

  20. Do KY, Fregien N, Pierce M, Cummings RD (1994) J Biol Chem 269: 23456–64.

    Google Scholar 

  21. Takeuchi M, Inoue N, Strickland TW, Kubota M, Wada M, Shimizu R, Hoshi S, Kozutsumi H, Takasaki S, Kobata A (1989) Proc Natl Acad Sci USA 86: 7819–22.

    Google Scholar 

  22. Misaizu T, Matsuki S, Strickland TW, Takeuchi M, Kobata A, Takasaki S (1995) Blood 86: 4097–104.

    Google Scholar 

  23. Yamamoto K, Ishida C, Shinohara Y, Hasegawa Y, Konami Y, Osawa T, Irimura T (1994) Biochemistry 33: 8159–66.

    Google Scholar 

  24. Powell LD, Varki A (1994) J Biol Chem 269: 10628–36.

    Google Scholar 

  25. Rice KG, Weisz OA, Barthel T, Lee RT, Lee YC (1990) J Biol Chem 265: 18429–34.

    Google Scholar 

  26. Patel TP, Goelz SE, Lobb RR, Parekh RB (1994) Biochemistry 33: 14815–24.

    Google Scholar 

  27. Dennis JW, Laferte S, Waghorne C, Breitman ML, Kerbel RS (1987) Science 236: 582–5.

    Google Scholar 

  28. Pierce M, Buckhaults P, Chen L, Fregien N (1997) Glycoconj J 14: 623–30.

    Google Scholar 

  29. Dabelsteen E (1996) J Pathol 179: 358–69.

    Google Scholar 

  30. Hiraizumi S, Taksaki S, Shiroki K, Kochibe N, Kobata A (1990) Arch Biochem Biophys 280: 9–19.

    Google Scholar 

  31. Endo T, Fujiwara T, Ikehara Y, Kobata A (1996) Eur J Biochem 236: 579–90.

    Google Scholar 

  32. Mizuochi T, Nishimura R, Derappe C, Taniguchi T, Hamamoto T, Mochizuki M, Kobata A (1983) J Biol Chem 258: 14126–9.

    Google Scholar 

  33. Endo T, Nishimura R, Kawano T, Mochizuki M, Kobata A (1987) Cancer Res 47: 5242–5.

    Google Scholar 

  34. Codogno P, Botti J, Font J, Aubery M (1985) Eur J Biochem 149: 453–60.

    Google Scholar 

  35. Granovsky M, Fode C, Warren CE, Campbell RM, Marth JD, Pierce M, Fregien N, Dennis JW (1995) Glycobiology 5: 797–806.

    Google Scholar 

  36. Yoshida T, Takahashi N, Nakashima I (1991) Mol Immunol 28: 1121–30.

    Google Scholar 

  37. Lemaire S, Derappe C, Michalski JC, Aubery M, Neel D (1994) J Biol Chem 269: 8069–74.

    Google Scholar 

  38. Minowa MT, Oguri S, Yoshida A, Hara T, Iwamatsu A, Ikenaga H, Takeuchi M (1998) J Biol Chem 273: 11556–62.

    Google Scholar 

  39. Yoshida A, Minowa MT, Hara T, Takamatsu S, Oguri S, Ikenaga H, Takeuchi M (1999) Glycobiology 9: 303–10.

    Google Scholar 

  40. Kumar R, Yang J, Larsen RD, Stanley P (1990) Proc Natl Acad Sci USA 87: 9948–52.

    Google Scholar 

  41. Sarkar M, Hull E, Nishikawa Y, Simpson RJ, Moritz RL, Dunn R, Schachter H (1991) Proc Natl Acad Sci USA 88: 234–8.

    Google Scholar 

  42. Kumar R, Yang J, Eddy RL, Byers MG, Shows TB, Stanley P (1992) Glycobiology 2: 383–93.

    Google Scholar 

  43. D'Agostaro GAF, Zingoni A, Moritz RL, Simpson RJ, Schachter H, Bendiak B (1995) J Biol Chem 270: 15211–21.

    Google Scholar 

  44. Tan J, D'Agostaro GAF, Bendiak B, Reck F, Sarkar M, Squire JA, Leong P, Schachter H (1995) Eur J Biochem 231: 317–28.

    Google Scholar 

  45. Nishikawa A, Ihara Y, Hatakeyama M, Kangawa K, Taniguchi N (1992) J Biol Chem 267: 18199–204.

    Google Scholar 

  46. Ihara Y, Nishikawa A, Tohma T, Soejima H, Niikawa N, Taniguchi N (1993) J Biochem (Tokyo) 113: 692–8.

    Google Scholar 

  47. Shoreibah M, Perng G-S, Adler B, Weinstein J, Basu R, Cupples R, Wen D, Browne JK, Buckhaults P, Fregien N, Pierce M (1993) J Biol Chem 268: 15381–5.

    Google Scholar 

  48. Saito H, Nishikawa A, Gu J, Ihara Y, Soejima H, Wada Y, Sekiya C, Niikawa N, Taniguchi N (1994) Biochem Biophys Res Commun 198: 318–27.

    Google Scholar 

  49. Bierhuizen MF, Fukuda M (1992) Proc Natl Acad Sci USA 89: 9326–330.

    Google Scholar 

  50. Bierhuizen MF, Mattei MG, Fukuda M (1993) Genes Dev. 7 468–78.

    Google Scholar 

  51. Sasaki K, Kurata-Miura K, Ujita M, Angata K, Nakagawa S, Sekine S, Nishi T, Fukuda M (1997) Proc Natl Acad Sci USA 94: 14294–9.

    Google Scholar 

  52. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) J Mol Biol 215: 403–10.

    Google Scholar 

  53. Yoshida A, Hara T, Ikenaga H, Takeuchi M (1995) Glycoconj J 12: 824–8.

    Google Scholar 

  54. Sambrook J, Fritsch EF, Maniatis T (eds) (1989) Molecular Cloning: A Laboratory Manual, 2nd edition. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.

    Google Scholar 

  55. Arcari P, Martinelli R, Salvatore F (1984) Nucleic Acids Res 12: 9179–89.

    Google Scholar 

  56. Ronin C, Bouchilloux S, Granier C, van Rietschoten J (1978) FEBS Lett 96: 179–82.

    Google Scholar 

  57. Kyte J, Doolittle RF (1982) J Mol Biol 157: 105–32.

    Google Scholar 

  58. Paulson JC, Colley KJ (1989) J Biol Chem 264: 17615–8.

    Google Scholar 

  59. Kleene R, Berger EG (1993) Biochim Biophys Acta 1154: 283–325.

    Google Scholar 

  60. Taniguchi N, Ihara Y (1995) Glycoconj J 12: 733–8.

    Google Scholar 

  61. Bakker H, Agterberg M, Van Tetering A, Koeleman CA, Van den Eijnden DH, Van Die I (1994) J Biol Chem 269: 30326–33.

    Google Scholar 

  62. Wang Y, Wong SS, Fukuda MN, Zu H, Liu Z, Tang Q, Appert HE (1994) Biochem Biophys Res Commun 204: 701–9.

    Google Scholar 

  63. Holmes EH, Xu Z, Sherwood AL, Macher BA (1995) J Biol Chem ]270: 8145–51.

    Google Scholar 

  64. Moremen KW, Robbins PW (1991) J Cell Biol 115: 1521–34.

    Google Scholar 

  65. Tjoelker LW, Seyfried CE, Eddy RL Jr, Byers MG, Shows TB, Calderon J, Schreiber RB, Gray PW (1994) Biochemistry 33: 3229–36.

    Google Scholar 

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Authors and Affiliations

  1. Central Laboratories for Key Technology, Kirin Brewery Co., Ltd., 1-13-5, Fukuura, Kanazawa-ku, Yokohama, 236-0004, Japan

    Aruto Yoshida, Mari T. Minowa, Shinji Takamatsu, Tomoka Hara, Hiroshi Ikenaga & Makoto Takeuchi

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  1. Aruto Yoshida
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  2. Mari T. Minowa
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  3. Shinji Takamatsu
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  4. Tomoka Hara
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  5. Hiroshi Ikenaga
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  6. Makoto Takeuchi
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Yoshida, A., Minowa, M.T., Takamatsu, S. et al. A novel second isoenzyme of the human UDP-N-acetylglucosamine:α1,3-D-mannoside β1,4-N-acetylglucosaminyltransferase family: cDNA cloning, expression, and chromosomal assignmen. Glycoconj J 15, 1115–1123 (1998). https://doi.org/10.1023/A:1006951519522

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