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Activity and adsorption of lipase from Nigella sativa seeds on Celite at different pH values

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Abstract

Lipase from Nigella sativa seeds was immobilized by adsorption on Celite 535 from phosphate buffer solutions varying pH values of 5.0–8.0 at 25 °C. Langmuir isotherms described the adsorption equilibria well for lipase adsorption at all pH range. The saturation capacity for adsorption of lipase increased from 14.5 to 24.3 mg g−1 Celite as the adsorption pH was reduced from 8 to 5, but the adsorption equilibrium constant remained constant and was determined to be 1.92 × 105 M−1. The adsorbed enzymes showed different activity values depending on the pH of the adsorption medium. The immobilized enzymes prepared at pH 6 displayed the highest activity values.

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References

  • Bosley JA, Peilow AD (1997) Immobilization of lipases on porous polypropylene: reduction in esterification efficiency at low loading. J. Amer. Oil Chem. Soc. 74: 107-111.

    Google Scholar 

  • Chaplin MF, Bucke C (1990) Enzyme Technology. Cambridge: Cambridge University Press, p. 16.

    Google Scholar 

  • Cui YM, Wei DZ, Yu JT (1997) Lipase-catalyzed esterification in organic solvent to resolve racemic naproxen. Biotechnol. Lett. 19: 865-868.

    Google Scholar 

  • Dandik L, Aksoy HA (1996) Application of Nigella sativa seed lipase in oleochemical reactions. Enzyme Microb. Technol. 19: 277-281.

    Google Scholar 

  • Gonçalves APV, Lopes JM, Lemos F, Ribeiro FR, Prazeres DMF, Cabral JMS, Aires-Barros MR (1997) Effect of the immobilization support on the hydrolytic activity of a cutinase from Fusarium solani pisi. Enzyme Microb. Technol. 20: 93-101.

    Google Scholar 

  • Karakazan S (1997) Corekotu (Nigella sativa L.) tohumlarindan lipaz enziminin ekstraksiyonu ve karakterizasyonu [Extraction and characterization of lipase from black cumin (Nigella sativa L.) seeds]. M. Sci. Thesis, Istanbul Technical University, Chemical Engineering Department, Istanbul (in Turkish).

    Google Scholar 

  • Kerzel P, Wenzig E, Mersmann A (1992) Investigation of the influence of several parameters on the adsorption of lipase. In: DECHEMA Biotechnology Conferences 5. VCH Verlagsgesellschaft, pp. 653-657.

  • Lee KT, Akoh CC (1998) Structured lipids: synthesis and applications. Food Rev. Int. 14: 17-34.

    Google Scholar 

  • Li ZY, Ward OP (1993) Enzyme catalyzed production of vegetable oils containing omega-3 polyunsaturated fatty acids. Biotechnol. Lett. 15: 185-188.

    Google Scholar 

  • Malcata FX, Reyes HR, Garcia HS, Hill CG, Amundson CH (1990) Immobilized lipase reactors for modification of fats and oils. A review. J. Amer. Oil Chem. Soc. 67: 890-910.

    Google Scholar 

  • Rajendran S, Sudhindra Rao K, Prakash V (1990) Effect of pH in the acidic region on the structural integrity of lipase from wheat germ. Indian J. Biochem. Biophys. 27: 300-310.

    Google Scholar 

  • Reetz MT, Zonda A, Simpelkamp J (1996) Efficient immobilization of lipases by entrapment in hydrophobic sol-gel materials. Biotechnol. Bioeng. 49: 527-534.

    Google Scholar 

  • Ustun G, Turkay S, Karaali A (1998) Nigella sativa seeds: a potential source for oils and oleochemicals. In: Koseoglu SS, Rhee KC, Wilson RF, eds. Proceedings of the World Conference on Oilseed and Edible Oils Processing, Vol. 2. Champaign: AOCS Press, pp. 155-160, ISBN 0-935315-84-2.

    Google Scholar 

  • Warmuth W, Wenzig E, Mersmenn A (1995) Selection of a support for immobilization of a microbial lipase for the hydrolysis of triglycerides. Bioprocess Eng. 12: 87-93.

    Google Scholar 

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Akova, A., Ustun, G. Activity and adsorption of lipase from Nigella sativa seeds on Celite at different pH values. Biotechnology Letters 22, 355–359 (2000). https://doi.org/10.1023/A:1005668500716

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  • DOI: https://doi.org/10.1023/A:1005668500716

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