Abstract
Yeast phase lysate antigens prepared from different isolates of Blastomyces dermatitidis (T-58, dog-Tennessee; T-27, polar bear-Tennessee; ERC-2, dog-Wisconsin; ER-3, woodpile-Wisconsin) were compared with respect to the detection of antibodies (indirect enzyme-linked immunosorbent assay-ELISA, peroxidase system) in 126 serial serum specimens (pre-treatment, 30 and 60 dayspost-treatment with itraconazole) from 42 dogs with diagnosed blastomycosis. Mean absorbance values observed with the four lysate antigens at the three treatment intervals ranged from the most reactive to the least reactive as follows: T-58 (0.270, 0.210, 0.136); T-27 (0.209, 0.156, 0.096); ER-3 (0.189, 0.144, 0.089) and ERC-2(0.158, 0.129, 0.080). Even though variations in reactivity were evidenced, the lysates prepared from isolates from various geographical regions and sources were all efficacious as antigens for the immunodiagnosis of canine blastomycosis.
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References
Bradsher RW. Blastomycosis. Clin Infect Dis 1992; 14: S82–S90.
Bradsher RW. Clinical features of blastomycosis. Semin Respir Infect 1997; 12: 206–218.
Davies SF, Sarosi GA. Epidemiological and clinical features of pulmonary blastomycosis. Semin Respir Infect 1997; 12: 206–218.
Murphy PA. Blastomycosis. JAMA 1989; 261: 3159–3161.
DiSalvo AF. The epidemiology of Blastomyces dermatitidis. In: Al-Doory Y, DiSalvo AF, eds. Blastomycosis. New York: Plenum Publishing Corp, 1992; 83–90.
DiSalvo AF. The ecology of Blastomyces dermatitidis. In: Al-Doory Y, DiSalvo AF, eds. Blastomycosis. New York: Plenum Publishing Corp, 1992; 43–69.
Klein BS, Vergeront JM, Davis JP. Epidemiological aspects of blastomycosis, the enigmatic mycosis. Sem Respir Infect 1986; 1: 29–39.
DeGrote M, Bjerke R, Smith H, Rhodes III LV. Expanding epidemiology of blastomycosis: Clinical features and investigation of 2 cases in Colorado. Clin Infect Dis 2000; 30: 582-584.
Dixon DM, McNeil MM, Choen ML, Gellin BG, LaMontagne JR. Fungal infections: A growing threat. Public Health Res 1996; 111: 226–235.
Sternberg S. The emerging fungal threat. Science 1994: 266: 1632–1634.
Goodman NL. Diagnosis of blastomycosis. In: Al-Doory Y, DiSalvo AF, eds. Blastomycosis. New York: Plenum Publishing Corp, 1992; 105–121.
Turner S, Kaufman L. Immunodiagnosis of blastomycosis. Semin Respir Infect 1986; 1: 22–28.
Kaufman L. Laboratory methods for the diagnosis and con-firmation of systemic mycoses. Clin Infect Dis 1992; 14: S23–S29.
Hurst SF, Kaufman L. Western immunoblot analysis and serologic characterization of Blastomyces dermatitidis yeast form extracellular antigens. J Clin Microbiol 1992; 30: 3043–3049.
Klein BS, Squires RA, Lloyd JK, Ruge DR, Legendre AM. Canine antibody response to Blastomyces dermatitidis WI-1 antigen. Am J Vet Res 2000; 61 (5): 554–558.
Johnson SM, Scalarone GM. Preparation and ELISA evaluation of Blastomyces dermatitidis yeast phase ysate antigens. Diagn Microbial Infect Dis 1988; 11: 81–86.
Bono JL, Legendre AM, Scalarone GM. Detection of antibodies and delayed hypersensitivity with Rotofor preparative IEF fractions of Blastomyces dermatitidis yeast phase lysate antigens. J Med Vet Mycol 1995; 33: 209–214.
Fisher MA, Bono JL, Abuodeh RO, Legendre AM, Scalarone GM. Sensitivity and specificity of an isoelectric focusing fraction of Blastomyces dermatitidis yeast lysate antigen for the detection of canine blastomycosis. Mycoses 1995; 38: 177–182.
Wakamoto A, Abuodeh RO, Scalarone GM. Comparative studies on the detection of antibodies and delayed hypersensitivity responses with 10 Blastomyces dermatitidis lysate antigens. Mycoses 1997; 40: 147–152.
Roomiany PL, Axtell RC, Scalarone GM. Comparison of seven Blastomyces dermatitidis antigens for the detection of antibodies in humans with occupationally acquired blastomycosis. Mycoses 2002; 46: 1–5.
Axtell RC, Scalarone GM. Serological differences in two Blastomyces dermatitidis isolates from different geographical regions of North America. Mycopathologia 2002; 153: 141–144.
Baumgardner DJ, Paretsky DP. Identification of Blastomyces dermatitidis in the stool of a dog with acute pulmonary blastomycosis. J Med Vet Mycol 1997; 35: 419–421.
Baumgardner DJ, Paretsky DP. The in vitro isolation of Blastomyces dermatitidis from a woodpile in north central Wisconsin, USA. Med Mycol 1999; 37: 163–168.
Levine HB, Scalarone GM, Chaparas SD. Preparation of fungal antigens and vaccines: Studies on Coccidioides immitis and Histoplasma capsulatum. Contrib Microbiol Immunol 1977; 3: 106–125.
Scalarone GM, Levine HB, Chaparas SD. Delayed hypersensitivity responses of experimental animals to histoplasmin from the yeast and mycelial phases of Histoplasma capsulatum. Infect Immun 1978; 21: 705–713.
Levine HB, Scalarone GM, Campbell GD, Graybill JR, Kelly PC, Chaparas SD. Histoplasmin-CYL, a yeast phase reagent in skin test studies in humans. Am Rev Respir Dis 1979; 119: 629–636.
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Chester, E., Axtell, R. & Scalarone, G. Blastomyces dermatitidis Lysate Antigens: Antibody Detection in Serial Serum Specimens from Dogs with Blastomycosis. Mycopathologia 156, 289–294 (2003). https://doi.org/10.1023/B:MYCO.0000003575.04692.2f
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DOI: https://doi.org/10.1023/B:MYCO.0000003575.04692.2f