Abstract
A lectin with a high affinity for glucose/mannose was isolated from Annona muricata seeds (Annonaceae) by gel filtration chromatography on Sephacryl S-200, ion exchange chromatography on a DEAE SP-5 PW column, and molecular exclusion on a Protein Pak Glass 300 SW column. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (PAGE) yielded two protein bands of approximately 14 kDa and 22 kDa. However, only one band was seen in native PAGE. The M r of the lectin estimated by fast-performance liquid chromatography–gel filtration on Superdex 75 was 22 kDa. The lectin was a glycoprotein with 8% carbohydrate (neutral sugar) and required divalent metal cations (Ca2+, Mg2+, and Mn2+) for full activity. Amino acid analysis revealed a large content of Glx, Gly, Phe, and Lys. The lectin agglutinated dog, chicken, horse, goose, and human erythrocytes and inhibited the growth of the fungi Fusarium oxysporum, Fusarium solani, and Colletotrichum musae.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Allen, A. K., Neuberger, A., and Sharon, N. (1973). Biochem. J. 131: 155–162.
Bories, C., Loiseau, P., Cortes, D., Myint, S. H., Hocquemiller, R., Gayral, P., et al. (1991). Plant Med. 57: 434–436.
Bradford, M. M. (1976). Anal. Biochem. 72: 248–254.
Branch, L. C., and Silva, I. M. F. (1983). Acta Amazônica. 13: 737–797.
Coelho, M. B., Freire, M. G. M., Toyama, M. H., Marangoni, S., Novello, J. C., and Macedo, M. L. R. (2003). Protein and Peptide Letter 10: 1–9.
Datta, P. K., Figueroa, M. O. D. C. R., and Lajolo, F. M. (1991). Plant Physiol. 97: 856–862.
Dubois, M., Gilles, K. A., Hamilton, J. L., Rebers, P. A., and Smith, F. (1956). Anal. Chem. 28: 350–356.
Freire, M. G. M., Gomes, V. M., Corsini, R. E., Machado, O. L. T., De Simone, S. G., Novello, J. C., et al. (2002). Plant Physiol. Biochem. 40: 61–68.
Goldstein, I. J., Hughes, R. C., Monsigny, M., Osawa, T., and Sharon, N. (1980). Nature 285: 66–73.
Gomes, V. M., Da Cunha, M., Miguens, F. C., Fernandes, K. V. S., Rose, T. L., and Xavier-Filho, J. (1998). Plant Sci. 138: 81–89.
Henrikson, R. L., and Meredith, S. C. (1984). Anal. Biochem. 136: 65–71.
Laemmli, U. K. (1970). Nature 227: 680–685.
Lis, H., and Sharon, N. (1984). In: Ginsburg, V., and Robbins, P. W. (eds), Biology of Carbohydrates, Wiley, New York, pp. 1–85.
Lis, H., and Sharon, N. (1986). Annu. Rev. Biochem. 55: 35–67.
Moreira, R. A., Monteiro, A. C. O., Horta, A. C. G., Oliveira, J. T. A., and Cavada, B. S. (1997). Phytochemistry 2: 139–144.
Moreira, R. A., Castelo-Branco, C. C., Monteiro, A. C. O., Tavares, R. O., and Beltramini, L. M. (1998). Phytochemistry 47: 1183–1188.
Peumans, W. J., Verhaert, P., Pfüller, U., and Van Damme, E. J. M. (1984). FEBS Lett. 177: 261–265.
Sampietro, A. R., Isla, M. I., Quiroga, E. N., and Vattuone, M. A. (2001). Plant Sci. 160: 659–667.
Sharon, N., and Lis, H. (1990). FASEB J. 4: 3198–3208.
Shibuya, N., Goldstein, I. J., Shafer, J. A., Peumans, W. J., and Broekaert, W. F. (1986). Arch. Biochem. Biophys. 249: 215–224.
Singh, R. S., Tiwary, A. K., and Kennedy, J. F. (1999). Crit. Rev. Biotechnol. 19: 145–178.
Utarabhand, P., and Akkayamont, P. (1995). Phytochemistry 38: 281–285.
Van Damme, E. J. M., Peumans, W. J., Barre, A., and Rougé, P. (1998). Crit. Rev. Plant. Sci. 17: 575–692.
Van Parijs, J., Broekaert, W. F., Goldstein, I. J., and Peumans, W. J. (1991). Planta 183: 258–264.
Van Parijs, J., Broekaert, W. F., Peumans, W. J., Geuns, J. M., and Van Laere, A. J. (1992). Arch. Microbiol. 158: 19–25.
Wang, H. X., Ng, T. B., and Ooi, V. E. C. (1999). Int. J. Biochem. Cell Biol. 31: 595–599.
Xu, Q., Lu, Y., Wang, X., Gu, H., and Chen, Z. (1998). Plant Physiol. Biochem. 36: 899–905.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Damico, D.C.S., Freire, M.G.M., Gomes, V.M. et al. Isolation and Characterization of a Lectin from Annona muricata Seeds. J Protein Chem 22, 655–661 (2003). https://doi.org/10.1023/B:JOPC.0000008730.50675.de
Published:
Issue Date:
DOI: https://doi.org/10.1023/B:JOPC.0000008730.50675.de