Abstract
Lipases from different sources (porcine pancreas,Mucor mieheiand Candida antarctica B) were covalently immobilized on a hydrophilic polyurethane composite (CoFoam). Their hydrolytic activities assayed with tributyrin were 0.55, 2.1 and 447 U g−1, respectively. The activity of the C. antarctica B composite in the synthesis of methyl oleate in hexane was 8.8 U g−1 compared to 60.6 U g−1 for commercial Novozyme 435. The advantage of the CoFoam composite lies in the low pressure drop in a packed-bed reactor at fairly large flow rates. For example, at flow rates of 10–12 l min−1, the pressure drop over 15 cm is typically 3 kPa.
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Vasudevan, P., López-Cortés, N., Caswell, H. et al. A novel hydrophilic support, CoFoam, for enzyme immobilization. Biotechnology Letters 26, 473–477 (2004). https://doi.org/10.1023/B:BILE.0000019553.26201.04
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DOI: https://doi.org/10.1023/B:BILE.0000019553.26201.04