Abstract
The Pichia pastoris expression system is widely used for the production of recombinant proteins. A simple and efficient experimental set-up allowing on-line monitoring of the methanol concentration during the fermentation of P. pastorisbased on the detection of the methanol vapor concentration in the exhaust air from fermenter by a tin dioxide (SnO2) semiconductor sensor is described. An experimental procedure to allow precise calibration of the system and to reduce methanol sensor's interferences (>95% reduction) are also presented and discussed. Accuracy and measurement error were estimated about 0.05 g ⋅ l−1 and 6%, respectively. The efficient monitoring of methanol will help to advanced control of recombinant protein production and process optimization.
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Ramon, R., Feliu, J., Cos, O. et al. Improving the monitoring of methanol concentration during high cell density fermentation of Pichia pastoris . Biotechnology Letters 26, 1447–1452 (2004). https://doi.org/10.1023/B:BILE.0000045649.60508.c5
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DOI: https://doi.org/10.1023/B:BILE.0000045649.60508.c5