Abstract
H2 production by Petrotoga miotherma, Thermosipho africanus, Thermotoga elfii, Fervidobacterium pennavorans, and Thermotoga neapolitana was compared under microaerobic conditions. Contrary to these previously reported strains being strict anaerobes, all tested strains grew and produced H2 in the presence of micromolar levels of O2. T. neapolitana showed the highest H2 production under these conditions. Microscopic counting techniques were used to determine growth curves and doubling times, which were subsequently correlated with optical density measurements. The Biolog anaerobic microtiter plate system was used to analyze the carbon source utilization spectrum of T. neapolitana and to select non-metabolized or poorly metabolized carbohydrates as physiological buffers. Itaconic acid was successfully used as a buffer to overcome pH-induced limitations of cell growth and to facilitate enhanced production of CO-free H2.
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References
Adams MW(1990) The metabolism of hydrogen by extremely thermophilic sulfur-dependent bacteria. FEMS Microbiol. Rev. 75: 219-238.
Balch WE, Wolfe RS (1976) New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HSCoM)-dependent growth of Methanobacterium ruminatiumin a pressurized atmosphere. Appl. Environ. Micribiol. 32: 781-791.
Baughn AD, Malamy MH (2004) The strict anaerobe Bacteroides fragilisgrows in and benefits from nanomolar concentrations of oxygen. Nature 427: 441-444.
Belkin S, Wirsen CO, Jannasch, HW (1986) A new sulfur-reducing, extremely thermophilic eubacterium from a submarine thermal vent. Appl. Environ. Microbiol. 51: 1180-1184.
Childers SE, Vargas M, Noll, KM (1992) Improved methods for the cultivation of the extremely thermophilic bacterium Thermotoga neapolitana. Appl. Environ. Microbiol. 58: 3949-3953.
Dobranik JK, Zak JC (1999) A microtiter plate procedure for evaluating fungal functional diversity. Mycologia 91: 756-765.
Huber R, Langworthy TA, Konig H, Thomm M, Woese CR, Sleytr UB, Stetter KO (1986) Thermotoga maritimasp. nov. represents a new genus of unique extremely thermophilic eubacteria growing up to 90 ?C. Arch. Microbiol. 144: 324-333.
Larminie J, Dicks A (2003) Fuel Cell Systems Explained.2nd edn., New York, NY: John Wiley & Sons.
Mahajan D (2003) A method for low temperature catalytic production of hydrogen. U.S. Patent # 6,596,423.
Preston-Mafham J, Boddy L, Randerson PF (2002) Analysis of microbial community functional diversity using sole-carbonsources utilisation profiles-a critique. FEMS Microbiol. Ecol. 42: 1-14.
Schroder C, Selig M, Schonheit P (1994) Glucose fermentation to acetate, CO2, and H2 in the anaerobic hyperthermophilic eubacterium Thermotoga maritima: involvement of the Embden-Meyerhof pathway. Arch. Microbiol. 161: 460-470.
Van Niel EWJ, Budde MAW, De Haas GG, Van der Wal FJ, Claassen PAM, Stams AJM (2002) Distinctive properties of high hydrogen producing extreme thermophiles,Caldicellulosiruptor saccharolyticusand Thermotoga elfii. Int. J. Hydrogen Energ. 27: 1391-1398.
Van Ooteghem SA, Beer SK, Yue PC (2002) Hydrogen production by the thermophilic bacterium Thermotoga neapolitana. Appl. Biochem. Biotechnol. 98-100: 177-189.
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Van Ooteghem, S.A., Jones, A., van der Lelie, D. et al. H2 production and carbon utilization by Thermotoga neapolitana under anaerobic and microaerobic growth conditions. Biotechnology Letters 26, 1223–1232 (2004). https://doi.org/10.1023/B:BILE.0000036602.75427.88
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DOI: https://doi.org/10.1023/B:BILE.0000036602.75427.88