Abstract
The degradation of 2,4-dinitrophenol (2,4-DNP) by Rhodococcus erythropolis HL PM-1 was studied. The enzymes involved in 2,4-DNP degradation were inducible, and their resynthesis took place during the process. Cell immobilization by embedding into agar gels decreased the degrader activity. The maximum rates of 2,4-DNP degradation by free and immobilized cells were 10.0 and 5.4 nmol/min per mg cells, respectively. The concentration dependence of 2,4-DNP degradation was typical of substrate inhibition kinetics. The immobilized cells were used in a model reactor designed for 2,4-DNP biodegradation. Its maximum capacity was 0.45 nmol/min per mg cells at a volumetric flow rate of 20 h–1. The reactor operated for 14 days without losing capacity; its half-life equaled 16 days.
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Kitova, A.E., Kuvichkina, T.N., Arinbasarova, A.Y. et al. Degradation of 2,4-Dinitrophenol by Free and Immobilized Cells of Rhodococcus erythropolis HL PM-1. Applied Biochemistry and Microbiology 40, 258–261 (2004). https://doi.org/10.1023/B:ABIM.0000025948.77233.dc
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DOI: https://doi.org/10.1023/B:ABIM.0000025948.77233.dc