Abstract
The effects of different plant growth regulators on in vitro adventitious shoot formation in Virginia pine (Pinus virginiana Mill.) zygotic embryo explants were quantitatively evaluated. Using Tang and Ouyang (1999) (TE) basal medium supplemented with 11.4 µM indole-3-acetic acid (IAA) and 2.2 µM N6-benzyladenine (BA), callus was observed after 3–6 weeks of culture. Calluses were transferred to TE basal medium supplemented with 0.49 µM indole-3-butyric acid (IBA) and 8.8 µM BA for 6–9 weeks, where they produced numerous small shoot primordia. They were then transferred to TE basal medium supplemented with 0.49 µM IBA and 4.4 µM BA to promote growth and elongation of adventitious shoots. After elongated shoots were transferred to TE medium containing 0.05 µM α-naphthaleneacetic acid (NAA) for 6 weeks, adventitious roots were formed. Regenerated plantlets were established in soil in greenhouse.
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Chang S, Sen S, McKinley CR, Aimers-Halliday J & Newton RJ (1991) Clonal propagation of Virginia pine (Pinus virginiana mill) by organogenesis. Plant Cell Rep. 10: 131–134
Gladfelter HJ & Phillips GC (1987) De novo shoot organogenesis of Pinus eldarica Med. in vitro. 1. Reproducible regeneration from long-term callus cultures.Plant Cell Rep. 6: 163–166
Jang JC & Taiter FH (1991) Micropropagation of shortleaf, Virginia and loblolly×shortleaf pine hybrids via organogenesis. Plant Cell Tiss. Org. Cult. 25: 61–67
Lambardi M, Harry IS, Menabeni D & Thorpe TA (1995) Organogenesis and somatic embryogenesis in Cupressus sempervirens. Plant Cell Tiss. Org. Cult. 40: 179–182
Park YS, Barrett JD & Bonga JM (1998) Application of somatic embryogenesis in high-value clonal forestry: deployment, genetic control, and stability of cryopreserved clones. In Vitro Cell Dev. Biol. Plant 34: 231–239
Tang W & Ouyang F (1999) Plant regeneration via organogenesis from six families of loblolly pine. Plant Cell Tiss. Org. Cult. 58: 223–226
Thorpe TA & Murashige T (1970) Some histochemical change underlying shoot initiation in tobacco callus cultures. Can. J. Bot. 48: 277–284
Von Aderkas P, Rohr R, Sundberg B, Gutmann M, Dumont-BeBoux N & Lelu MA (2002) Abscisic acid and its influence on development of the embryonal root cap, storage product and secondary metabolite accumulation in hybrid larch somatic embryos. Plant Cell Tiss. Org. Cult. 69: 111–120
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Tang, W., Harris, L.C., Outhavong, V. et al. The Effect of Different Plant Growth Regulators on Adventitious Shoot Formation from Virginia Pine (Pinus virginiana) Zygotic Embryo Explants. Plant Cell, Tissue and Organ Culture 78, 237–240 (2004). https://doi.org/10.1023/B:TICU.0000025658.73970.57
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DOI: https://doi.org/10.1023/B:TICU.0000025658.73970.57