Abstract
The effect of low concentrations of O2 (1%) with or without the application of exogenous ethylene (10 μl/l) on the production of endogenous ethylene, the activity of polygalacturonase (PG), and the ripening of tomato fruits during storage for three weeks at 20°C and four weeks at 10°C, followed by one week under ambient conditions (25°C) was studied. The internal ethylene concentration in the fruits stored under low O2 at 10 or 20°C was low during storage and increased only when fruits were transferred to ambient conditions. The application of exogenous ethylene to fruits stored under low O2 at 10 or 20°C did not induce autocatalytic ethylene synthesis. By contrast, the internal ethylene concentration of fruits stored in air was high at 20°C and somewhat lower at 10°C. Under low O2 conditions, PG activity was low and the fruits remained firm and green throughout storage, whereas, during storage in the air, PG activity increased and the fruits softened and developed their characteristic red color.
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REFERENCES
Lyons, J.M. and Pratt, H.K., Effects of Stage of Maturity and Ethylene Treatment on Respiration and Ripening of Tomato Fruit, J. Am. Soc. Hort. Sci., 1964, vol. 84, pp. 491-500.
Lieberman, M., Biosynthesis and Action of Ethylene, Annu. Rev. Plant Physiol., 1979, vol. 30, pp. 533-591.
Burg, S.P., The Physiology of Ethylene Formation, Annu. Rev. Plant Physiol., 1962, vol. 13, pp. 265-302.
Burg, S.P. and Burg, E.A., Ethylene Action and the Ripening of Fruits, Science, 1965, vol. 148, pp. 1190-1196.
Ryall, A.L. and Lipton, W.J., Handling, Transportation and Storage of Fruits and Vegetables, vol. 1, Vegetables and Melons, Westport: Avi, 1979.
Hobson, G.E., The Short-Term Storage of Tomato Fruit, J. Hort. Sci., 1981, vol. 56, pp. 363-368.
Rushing, J.W. and Huber, D.J., In Vitro Characterization of Tomato Fruit Softening, Plant Physiol., 1984, vol. 75, pp. 891-894.
Burg, S.P. and Burg, E.A., Molecular Requirements for the Biological Activity of Ethylene, Plant Physiol., 1967, vol. 42, pp. 144-152.
Yang, S.F., Biosynthesis of Ethylene and Its Regulation, Recent Advantages in the Biochemistry of Fruit and Vegetables, Friend, J. and Rhodes, M.J.C., Eds., London: Academic, 1981, pp. 89-106.
Legge, R.L. and Thompson, J.E., Involvement of Hydroperoxides and an ACC-Derived Free Radical in the Formation of Ethylene, Phytochemistry, 1983, vol. 22, pp. 2161-2166.
Hobson, G.E., Richardson, C., and Gillham, D.J., Release of Protein from Normal and Mutant Tomato Cell Walls, Plant Physiol., 1983, vol. 71, pp. 635-638.
Yang, S.F., Biosynthesis and Action of Ethylene, Hort-Science, 1985, vol. 20, pp. 41-45.
Lidster, P.D., McRae, K.B., and Sanford, K.A., Responses of “McIntosh” Apples to Low Oxygen Storage, J. Am. Soc. Hort. Sci., 1981, vol. 106, pp. 159-162.
Kapotis, G.E., Lougheed, E.C., and Miller, S.R., Low-O2 Storage—Can It Be?, Controlled Atmospheres for Storage and Transport of Perishable Agricultural Commodities, Richardson, D.G. and Meheriuk, M., Eds., Oregon: Timber Press, 1982, pp. 121-129.
Sfakiotakis, E., Niklis, N., Stavroulakis, G., and Vassiliadis, T., Efficacy of Controlled Atmosphere and Ultra Low Oxygen—Low Ethylene Storage on Keeping Quality and Scald Control of “Starking Delicious” Apples, Acta Hort., 1993, vol. 326, pp. 191-202.
Mellenthin, W.M., Chen, P.M., and Kelly, S.B., Low Oxygen Effects on Dessert Quality, Scald Prevention and Nitrogen Metabolism of d'Anjou Pear Fruit During Long-Term Storage, J. Am. Soc. Hort. Sci., 1980, vol. 105, pp. 522-527.
Tigchelaar, E.C., McGlasson, W.B., and Buescher, R.W., Genetic Regulation of Tomato Fruit Ripening, Hort-Science, 1978, vol. 13, pp. 508-513.
Kanellis, A.K., Solomos, T., and Matoo, A.K., Hydrolytic Enzyme Activities and Protein Pattern of Avocado Fruit Ripened in Air and in Low Oxygen, with and without Ethylene, Plant Physiol., 1989, vol. 90, pp. 259-264.
Sozzi, G.O., Trinchero, G.D., and Fraschina, A.A., Controlled-Atmosphere Storage of Tomato Fruit: Low Oxygen or Elevated Carbon Dioxide Levels Alter Galacturonase Activity and Inhibit Exogenous Ethylene Action, J. Sci. Food Agric., 1999, vol. 8, pp. 1065-1070.
Kapotis, G.E., Factors Affecting the Ripening and Post-Harvest Life of Tomato Fruit, Doctoral Dissertation, Agricultural University of Athens, 2001.
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Kapotis, G., Passam, H.C., Akoumianakis, K. et al. Storage of Tomatoes in Low Oxygen Atmospheres Inhibits Ethylene Action and Polygalacturonase Activity. Russian Journal of Plant Physiology 51, 112–115 (2004). https://doi.org/10.1023/B:RUPP.0000011310.84965.74
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DOI: https://doi.org/10.1023/B:RUPP.0000011310.84965.74