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Dual positional specificity and expression of non-traditional lipoxygenase induced by wounding and methyl jasmonate in maize seedlings

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Abstract

Lipoxygenases (LOXs) catalyze the formation of fatty acid hydroperoxides involved in responses to stresses. This study examines the expression of a non-traditional dual positional specific maize LOX in response to wounding or methyl jasmonate (MeJA). Full-length maize LOX cDNA was expressed in Escherichia coli, and recombinant LOX was purified and characterized enzymatically. RP-HPLC and GC-MS analysis showed that the purified LOX converts α-linolenic acid into 13-hydroperoxylinolenic acid and 9-hydroperoxylinolenic acid in a 6:4 ratio. LOX mRNA accumulated rapidly and transiently in response to wounding reaching a peak of expression about 3 h after wounding. This increase followed an initial increase in endogenous jasmonic acid (JA) 1 h after wounding (JA burst). However, the expression of LOX induced by MeJA lasted longer than the expression induced by wounding, and the MeJA-induced expression seemed to be biphasic pattern composed of early and late phases. The expression of LOX in the presence of inhibitors of JA biosynthesis was not completely inhibited, but delayed in wound response and the expression period was shortened in MeJA response. These results suggest that wound-responsive JA burst may trigger the early phase of LOX expression which facilitates biosynthesis of endogenous JA through its 13-LOX activity, and subsequently leads to the activation of the late phase LOX expression in MeJA-treated maize seedlings. Implications of dual positional specificity of maize LOX in the observed expression kinetics are discussed.

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Authors and Affiliations

  1. Department of Applied Biotechnology, Agricultural Plant Stress Research Center, and Biotechnology Research Institute, Chonnam National University, Kwangju, 500-757, Republic of Korea

    Eun-Seon Kim, Eunyoung Choi, Youngsun Kim, Kyoungwon Cho, Ayoung Lee, Jaehan Shim & Oksoo Han

  2. Molecular and Microbial Ecology Research Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, 305-8566, Ibaraki, Japan

    Randeep Rakwal

  3. Plant Functional Genomics Laboratory (PFGL), National Institute of Agrobiological Sciences (NIAS), Kannondai 2-1-2, Tsukuba, 305-8602, Ibaraki, Japan

    Ganesh Kumar Agrawal

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  1. Eun-Seon Kim
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  2. Eunyoung Choi
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Correspondence to Oksoo Han.

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Kim, ES., Choi, E., Kim, Y. et al. Dual positional specificity and expression of non-traditional lipoxygenase induced by wounding and methyl jasmonate in maize seedlings. Plant Mol Biol 52, 1203–1213 (2003). https://doi.org/10.1023/B:PLAN.0000004331.94803.b0

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