Abstract
A plate method was developed to screen for xylose isomerase–producing microorganisms based on the use of 2,3,5-triphenyltetrazolium as an indicator of D-xylulose, the D-xylose isomerization product. The use of this method allows microorganisms to be differentiated by the character of the enzyme synthesis (inducible or constitutive).
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REFERENCES
Bhosale, S.H., Rao, M.B., and Deshpande, V.V., Molecular and Industrial Aspects of Glucose Isomerase, Microbiol. Rev., 1996, vol. 60,no. 2, pp. 280–300.
Walfridsson, M., Bao, X., Anderlund, M., Lilius, G., Bülow, L., and Hang-Hägerdal, B., Ethanolic Fermentation of Xylose with Saccharomyces cerevisiae Harboring the Thermus thermophilus xylA Gene, Which Expresses an Active Xylose (Glucose) Isomerase, Appl. Environ. Microbiol., 1996, vol. 62,no. 12, pp. 4648–4651.
Nakamura, M., Determination of Fructose in the Presence of a Large Excess of Glucose. V. A Modified Cysteine-Carbazole Reaction, Agric. Biol. Chem., 1968, vol. 32,no. 6, pp. 701–706.
Yamanaka, K., Methods Enzymol., 1975, vol. 41, p. 466.
Mosichev, M.S. and Kozhanova, S.P., Application of Immunoenzymatic Analysis in Screening for Glucose Isomerase Producers, Biotekhnologiya, 1986, no. 6, pp. 28–32.
Kersters-Hilderson, H., Callens, M., Van Opstal, O., Vangrysperre, W., and De Bruyne, C.K., Kinetic Characterization of D-Xylose Isomerases by Enzymatic Assays Using D-Sorbitol Dehydrogenase, Enzyme Microbiol. Technol., 1987, vol. 9, pp. 145–148.
Li Chkhun Sev, Express Screening for Glucose Isomerase Producers among Various Groups of Microorganisms, IV Vses. konf. “Biosintez fermentov mikroorganizmami,” Tez. dokl. (6th All-Union Conference “Enzyme Biosynthesis by Microorganisms,” Abstracts of Papers), 1988, p. 217.
Schenk, M. and Bisswanger, H., A Microplate Assay for D-Xylose/D-Glucose Isomerase, Enzyme Microbiol. Technol., 1998, vol. 22, pp. 721–723.
Sanchez, S. and Smiley, K.L., Properties of D-Xylose Isomerase from Streptomyces albus, Appl. Microbiol., 1975, vol. 29,no. 6, pp. 745–750.
Chen, W.P., Glucose Isomerase (a Review), Process Biochem., 1980, vol. 15,no. 5, pp. 30–35.
Stoichev, M., Ognyanov, I., and Dzhezheva, G., Podbor na perspektivni shchamove ot rod Streptomyces, produtsirashchi enzima glyukozoizomeraza, Acta Microbiol. Bulg., 1981, vol. 8, pp. 57–62.
Kwon, H.-J., Kitada, M., and Horikoshi, K., Purification and Properties of D-Xylose Isomerase from Alkalophilic Bacillus no. KX-6, Agric. Biol. Chem., 1987, vol. 51,no. 7, pp. 1983–1989.
Ananichev, A.V., Galitskaya, N.B., Ulezlo, I.V., and Egorov, A.M., Obtaining of Crystalline Fructose from a Glucose-Fructose Syrup, Prikl. Biokhim. Mikrobiol., 1985, vol. 21,no. 2, pp. 260–264.
Sarthy, A.V., McConaughy, B.L., Lobo, Z., Sundstrom, J.A., Furlong, C.E., and Hall, B.D., Expression of the Escherichia coli Xylose Isomerase Gene in Saccharomyces cerevisiae, Appl. Environ. Microbiol., 1987, vol. 53,no. 9, pp. 1996–2000.
Tashpulatova, B.A., Davranov, K.D., and Mezhlum'yan, L.G., Characterization of Glucose Isomerase of Streptomyces atratus, Khim. Prir. Soedin., 1991, no. 6, pp. 833–837.
Lee, C., Bagdasarian, M., Meng, M., and Zeikus, J.G., Catalytic Mechanism of Xylose (Glucose) Isomerase from Clostridium thermosulfurogenes, J. Biol. Chem., 1990, vol. 265, pp. 19082–19090.
Deshmukh, S.S. and Shankar, V., Glucose Isomerase from Thermophilic Streptomyces thermonitrificans: Purification and Characterization, Biotechnol. Appl. Biochem., 1996, vol. 24,no. 1, pp. 65–72.
Slater, T.P., Sawyer, B., and Sträuli, U., Studies on Succinate-Tetrazolium Reductase Systems. III. Points of Coupling of Four Different Tetrazolium Salts, Biochim. Biophys. Acta, 1963, vol. 77, pp. 383–393.
Sapunova, L.I., Kazakevich, I.O., and Parakhnya, E.V., Investigation of the Synthesis of Xylose/Glucose Isomerases by Six Arthrobacter sp. Strains, Mikrobiologiya, 2000, vol. 69,no. 5, pp. 647–652.
Sapunova, L.I., Parakhnya, E.V., Kazakevich, I.O., and Evtushenkov, A.N., The Use of 2,3,5-Triphenyltetrazolium for Qualitative Determination of Xylose Isomerase on Agarized Media Containing D-Xylose, Biotekhnologiya, 2003, no. 1, pp. 85–90.
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Sapunova, L.I., Lobanok, A.G., Kazakevich, I.O. et al. A Plate Method to Screen for Microorganisms Producing Xylose Isomerase. Microbiology 73, 107–112 (2004). https://doi.org/10.1023/B:MICI.0000016378.43654.0a
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DOI: https://doi.org/10.1023/B:MICI.0000016378.43654.0a