Abstract
The interactions of fluorophores with noble metal particles can modify their emission spectral properties, a relatively new phenomenon in fluorescence. We subsequently examined indocyanine green (ICG), which is widely used in medical testing and imaging, in close proximity to an electrically roughened platinum electrode. The emission intensity and lifetimes were decreased about 2-fold on the roughened surface as compared to a smooth Pt surface, and the photostability about the same. Platinum does not appear promising for metal enhanced fluorescence, at least for long wavelength fluorophores.
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J. R. Lakowicz (2001). Radiative decay engineering: Biophysical and biomedical applications. Anal. Biochem. 298, 1-24.
J. R. Lakowicz, Y. Shen, S. D'Auria, J. Malicka, J. Fang, Z. Gryczynski, and I. Gryczynski (2002). Radiative decay engineering 2. Effects of silver island films on fluorescence intensity, lifetimes, and resonance energy transfer. Anal. Biochem. 301, 261-277.
J. R. Lakowicz, Y. B. Shen, Z. Gryczynski, S. D'Auria, and I. Gryczynski (2001). Intrinsic fluorescence from DNA can be enhanced by metallic particles. Biochem. Biophys. Res. Commun. 286, 875-879.
J. Malicka, I. Gryczynski, J. Kusba, Y. B. Shen, and J. R. Lakowicz (2002). Effects of metallic silver particles on resonance energy transfer in labeled bovine serum albumin. Biochem. Biophys. Res. Commun. 294, 886-892.
C. D. Geddes, H. Cao, I. Gryczynski, Z. Gryczynski, J. Fang, and J. R. Lakowicz (2003). Metal-enhanced fluorescence (MEF) due to silver colloids on a planar surface: Potential applications of indocyanine green to in vivo imaging. J. Phys. Chem. A 107(18), 3443-3449.
R. R. Chance, A. Prock, and R. Silbey (1978). Molecular fluorescence and energy transfer near interfaces. Adv. Chem. Phys. 37, 1-65.
J. I. Gersten and A. Nitzan (1984). Accelerated energy transfer between molecules near a solid particle. Chem. Phys. Lett. 104,31-37.
J. Gersten and A. Nitzan (1981). Spectroscopic properties of molecules interacting with small dielectric particles. J. Chem. Phys. 75, 1139-1152.
C. D. Geddes, H. Cao, and J. R. Lakowicz (2003). Enhanced photostability of ICG in close proximity to gold colloids. Spectrochim. Acta: A 59(11), 2611-2617.
B. Dubertret, M. Calame, and A. J. Libchaber (2001). Single-mismatch detection using gold-quenched fluorescent oligonucleotides. Nat. Biotechnol. 19, 365-370.
M. Fleischmann, P. J. Hendra, and A. J. McQuillan (1974). Raman spectra of pyridine adsorbed at a silver electrode. Chem. Phys. Letts. 26(2), 163-166.
E. Roth, G. A. Hope, D. P. Schweinsberg, W. Kiefer, and P. M. Fredericks (1993). Simple technique for measuring surface-enhanced fourier transform Raman spectra of organic compounds. Appl. Spectrosc. 47(11), 1794-1800.
Q. B. Huanq, W. W. Cai, B. M. Mao, F. Liu, and Z. Tian (1996). Surface Raman spectra of pyridine and hydrogen on bare platinum electrodes. J. Electroanal. Chem. 415(1/2), 175-178.
P. Cao, G. Bin, and Z. Tian (2002). Surface enhanced raman scattering of pyridine on platinum and nickel electrodes in nonaqueous solutions. Chem. Phys. Lett. 366, 440-446.
T. M. Jovin and D. J. Arndt-Jovin (1989). in E. Kohen, J. G. Hirschberg, and J. S. Ploem (Eds.), Cell Structure and Function by Microscopyfluorometry, Academic Press. London, pp. 99-117.
J. Yguerabide and E. E. Yguerabide (1998). Light-scattering sub-microscopic particles as highly fluorescent analogs and their use as tracer labels in clincal and biological applications—1. Theory. Anal. Biochem. 262, 137-156.
J. Yguerabide and E. E. Yguerabide (1998). Light-scattering sub-microscopic particles as highly fluorescent analogs and their use as tracer labels in clincal and biological applications—2. Experimental characterization. Anal. Biochem. 262, 157-176.
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Geddes, C.D., Parfenov, A., Roll, D. et al. Fluorescence Spectral Properties of Indocyanine Green on a Roughened Platinum Electrode: Metal-Enhanced Fluorescence. Journal of Fluorescence 13, 453–457 (2003). https://doi.org/10.1023/B:JOFL.0000008055.22336.0b
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DOI: https://doi.org/10.1023/B:JOFL.0000008055.22336.0b