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Identification and Subcellular Localization of Neuronal Calcium Sensor-1 (NCS-1) in Human Neutrophils and HL-60 Cells

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Abstract

Secretion in neutrophils is thought to be regulated in different ways for the different granule types. Specific granules are endowed with proteins which are related to docking and fusion events and are absent on azurophilic granules. Furthermore, even if secretion of content from all neutrophil granules is a Ca2 +-dependent process, a higher concentration of cytosolic calcium is required for azurophilic than for specific granule secretion. In this paper we show that human neutrophils and promyelocitic cells express neuronal calcium sensor-1 (NCS-1), a calcium binding protein involved in exocytosis in various cell types. Both mRNA and protein were found in mature cells and precursors. NCS-1 is shown to be mainly associated with azurophilic granules and, therefore could play an instrumental role in the calcium-dependent secretion of azurophilic granules.

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Authors and Affiliations

  1. Dipartimento di Fisiologia e Patologia, Università di Trieste, Italy

    Cristiana Brochetta, Maria Giovanna Perrotta, Maurizio Romano, Francesca Vita, Maria Rosa Soranzo, Violetta Borelli & Giuliano Zabucchi

  2. Division Of Neurosciences, Baylor College of Medicine, Houston, Texas

    Andreas Jeromin

  3. Mount Sinai Hospital, Samuel Lunenfeld Research Institute, Toronto, Canada

    John Roder

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  1. Cristiana Brochetta
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  2. Maria Giovanna Perrotta
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  3. Andreas Jeromin
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  4. Maurizio Romano
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  5. Francesca Vita
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  9. Giuliano Zabucchi
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Correspondence to Giuliano Zabucchi.

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Brochetta, C., Perrotta, M.G., Jeromin, A. et al. Identification and Subcellular Localization of Neuronal Calcium Sensor-1 (NCS-1) in Human Neutrophils and HL-60 Cells. Inflammation 27, 361–372 (2003). https://doi.org/10.1023/B:IFLA.0000006704.13043.51

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