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Ultrastructural changes and diosgenin content in cell suspensions of Trigonella foenum-graecum L. by ethylene treatment

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Abstract

This paper studies the effect of different concentrations of ethephon (an ethylene releasing compound) on the level of the steroidal sapogenin diosgenin in cell suspensions of Trigonella foenum-graecum L. The results reveal that diosgenin synthesis and/or accumulation was stimulated by 5 ppm ethephon treatment, an increase of 126% being observed, while concentrations of 25 ppm and 50 ppm reduced the levels of this secondary compound. Changes in the isopentenyl diphosphate isomerase activity, increased cell diameter, decreased cell packing (with all the ethephon concentrations assayed), increased cytoplasmic density (5 ppm ethephon treatment) and alterations in the membrane structures (25 and 50 ppm ethephon treatments) were also observed.

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References

  • Beneytout J. L., Nappez C., Lebouet M. J. and Malinvaud G. 1995. A plant steroid, diosgenin, a new megakaryocytic differentiation inducer of HEL cells. Biochem. Biophys. Res. Commun. 207: 398–404.

    Article  PubMed  CAS  Google Scholar 

  • Berglund T. and Ohlsson A. B. 1992. Effects of ethylene and aminoethoxy vinyl-glycine on cardenolide accumulation in tissue cultures of Digitalis lanata. J. Plant Physiol. 140: 395–398.

    CAS  Google Scholar 

  • Berhow M. A. and Vandercook C. E. 1992. The reduction of naringin content of grapefruit by applications of gibberellic acid. Plant Growth Regul. 11: 75–80.

    Article  CAS  Google Scholar 

  • Borochov A., Spiegelstein H. and Philosoph-Hadas 1997. Ethylene and flower petal senescence: interrelationship with membrane lipid catabolism. Physiol. Plant. 100: 606–612.

    Article  CAS  Google Scholar 

  • Bradford M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.

    Article  PubMed  CAS  Google Scholar 

  • Brown G. D. 1998. The biosynthesis of steroids and triterpenoids. NPR. Covering 1990–1996.

  • Chiang H. C., Tseng T. H., Wang C. J., Chen C. P. and Kan W. S. 1991. Experimental antitumor agents from Solanum indicum L. Anticancer Res. 11: 1911–1917.

    PubMed  CAS  Google Scholar 

  • Cho G. H., Kim D. I., Pedersen H. and Chin C.-K. 1988. Ethephon enhancement of secondary metabolite synthesis in plant cell cultures. Biotechnol. Prog. 41: 84–188.

    Google Scholar 

  • Davisson V. J., Woodside A. B. and Poulter C. D. 1985. Synthesis of allylic and homoallylic isoprenoid pyrophosphates. Method. Enzymol. 110: 130–144.

    CAS  Google Scholar 

  • Del Río J. A., Ortuño A., García Puig D., Iborra J. L. and Sabater F. 1991. Accumulation of the sesquiterpenes nootkatone and valencene by callus cultures of Citrus paradisi, Citrus limonia and Citrus aurantium.Plant Cell Rep. 10: 410–413.

    Google Scholar 

  • Del Río J. A., Fuster M. D., Sabater F., Porras I., García Lidón A. and Ortuño A. 1995. Effect of benzylaminopurine on the flavanones hesperidin, hesperetin 7-O-Glucoside,and pruning in Tangelo Nova fruits. J. Agric. Food Chem. 43: 2030–2034.

    Article  Google Scholar 

  • García Puig D., Pérez M. L., Fuster M. D., Ortuño A., Sabater F., Porras I., García Lidón A. and Del Río J. A. 1995. Modification by ethylene of the secondary metabolites naringin,narirutin and nootkatone in grapefruit. Planta Med. 61: 283–285.

    Google Scholar 

  • Gaspar T., Kevers C., Penel C., Greppin H., Reid D. M. and Thorpe T. A. 1996. Plant hormones and plant growth regulators in plant tissue culture. In Vitro Cell Dev-Pl 32: 272–289.

    CAS  Google Scholar 

  • Ghosal S., Srivastava R. S., Chatterjee D. C. and Dutta S. K. 1974. Fenugreekine a new steroidal sapogenin peptide ester of Trigonella foenum-graecum. Phytochemistry 13: 2247–2252.

    Article  CAS  Google Scholar 

  • Lee C. W. and Shuler M. L. 1991. Different Shakeflask closure alter gas phase composition and ajmalicine production in Catharanthus roseus cell suspensions. Biotechnol Tech. 5: 173–178.

    Article  CAS  Google Scholar 

  • Liebermas S. J., Valdovinos J. G. and Jensen T. H. 1983. A morphometric study on the effects on ethylene treatment in promoting abscission of tobacco flower pedicels. Plant Physiol. 72: 583–585.

    Google Scholar 

  • Linden J. C., Haigh J. R., Mirjalili N. and Phisaphalong M. 2001. Gas concentration effects on secondary metabolite production by plant cell cultures. Adv. Biochem. Eng/Biotech. 72: 27–62.

    CAS  Google Scholar 

  • Liu B., Brian Lockwood G. and Gifford L. A. 1995. Supercriticalfluid extraction of diosgenin from tubers of Discorea nipponica. J. Chromatogr. A 690: 250–253.

    Article  CAS  Google Scholar 

  • Nappez C., Liagre B. and Beneytout J. L. 1995. Changes in lipoxygenase activities in human erythroleukemia (HEL)cells during diosgenin-induced differentiation. Cancer Lett. 96: 133–140.

    Article  PubMed  CAS  Google Scholar 

  • Oncina R., Botía J. M., Del Río J. A. and Ortuño A. 2000. Bioproduction of diosgenin in callus cultures of Trigonella foenumgraecum. Food Chem. 70: 489–492.

    Article  CAS  Google Scholar 

  • Ortuño A., Sánchez-Bravo J., Moral J. R., Acosta M. and Sabater F. 1990. Changes in the concentration of indole-3-acetic acid during the growth of etiolated lupin hypocotyls. Plant Physiol. 78: 211–217.

    Article  Google Scholar 

  • Ortuño A., Del Río J. A., Casas J. L., Serrano M., Acosta M. and Sánchez-Bravo J. 1991. Influence of ACC and ethephon on cell growth in etiolated lupin hypocotyls. Dependence on cell growth state. Biol. Plant. 33: 81–90.

    Google Scholar 

  • Ortuño A., García Puig D., Sabater F., Porras I., García Lidón A. and Del Río J. A. 1993. Influence of ethylene and ethephon on the sesquiterpene nootkatone production in Citrus paradisi. J. Agric. Food Chem. 41: 1566–1569.

    Article  Google Scholar 

  • Ortuño A., Oncina R., Botía J. M. and Del Río J. A. 1998. Distribution and changes of diosgenin during development of Trigonella foenum-graecum plants. Modulation by benzylaminopurine. Food Chem. 63: 51–54.

    Article  Google Scholar 

  • Ramos-Valdivia A. C., der Heijden R. and Verpoorte R. 1997. Elicitor-mediated induction of anthraquinone biosynthesis and regulation of isopentenyl diphosphate isomerase and farnesyl diphosphate synthase activities in cell suspension cultures in Cinchona robusta. Planta 203: 155–161.

    Article  CAS  Google Scholar 

  • Rokem J. S., Tal B. and Goldberg I. 1985. Methods for increasing diosgenin production by Dioscorea cells in suspension cultures. J. Nat. Prod. 48: 210–220.

    Article  CAS  Google Scholar 

  • Roman I. D., Thewles A. and Coleman R. 1995. Fractionation of livers following diosgenin treatment to elevate binary cholesterol. Biochem. Biophys. Acta 1255: 77–81.

    PubMed  Google Scholar 

  • Sánchez-Bravo J., Ortuño A., Pérez-Gilabert M., Acosta M. and Sabater F. 1992. Modification by ethylene of the cell growth pattern in different tissues of etiolated lupine hypocotyls. Plant Physiol. 98: 1121–1127.

    Article  PubMed  Google Scholar 

  • Sargent J. A. and Osborne D. J. 1975. An effect of ethylene on the endoplasmic reticulum of expanding cells of etiolated shoots of Pisum sativum. Planta 124: 199–206.

    Article  Google Scholar 

  • Satterwhite D. M. 1985. Isopentenyldiphosphate delta-isomerase. Method. Enzymol. 110: 92–99.

    Article  CAS  Google Scholar 

  • Sauvaire Y. and Baccou J. C. 1978. L 'obtention de la diosgénine. Problémes de l 'hydrolyse acide des saponines. Lloydia 41: 247–256.

    CAS  Google Scholar 

  • Sauvaire Y., Ribes G. and Baccou J. C. 1991. Loubatières Mariani, M. M. Implication of steroid saponins and sapogenins in the hypocholesterolemic effect of fenugreek. Lipids 26: 191–197.

    PubMed  CAS  Google Scholar 

  • Shibli R. A., Smith M. A. L. and Kushad M. 1997. Headspace ethylene accumulation effects on secondary metabolite production in Vaccinium pahalaecell culture. Plant Growth Regul. 23: 201–5.

    Article  CAS  Google Scholar 

  • Stepan-Sarkissian G. and Grey D. 1990. Growth determination and medium analysis. In: Pollard J. W. and Walker J. M. (eds), Methods in Molecular Biology. Plant Cell and Tissue Culture. Humana Press, 6: 13–27.

  • Vezina C. 1987. In: Bu'lock J. and Kristiansen B. (eds), Basic Biotechnology. Academic Press Inc., London, pp. 468–470.

    Google Scholar 

  • Warnecke D. C. and Heinz E. 1994. Purification of a membrane-bound UDP-glucose:sterol [beta]-D-glucosiltransferase based on its solubility in diethyl ether. Plant Physiol. 4: 1067–1073.

    Google Scholar 

  • Wilson C. W., Shaw P. E., McDonald R. E., Greany P. D. and Yokohama H. 1990. Effect of gibberellic acid and 2-(3,4-dichloro-phenoxy)triethylamine on nootkatone in grapefruit peel oil and total peel oil content. J. Agric. Food Chem. 38: 656–659.

    Article  CAS  Google Scholar 

  • Wu R. T., Lin W. J., Chiang H. C., Horhg L. Y. and Chung Y. M. 1990a. Modulation of experimental autoimmune uveitis with formosanin-C in guinea pigs. J. Ocul. Pharmacol. 4: 301–311.

    Article  Google Scholar 

  • Wu R. T., Chiang H. C., Fu W. C., Chien K. Y., Chun Y. M. and Horng L. Y. 1990b. Formosanin-C, an immunomodulator with antitumor activity. Int. J. Immunopharm. 7: 777–786.

    Google Scholar 

  • Zhang C. H. and Wu J. Y. 2003. Ethylene inhibitors enhance elicitor-induced paclitaxel production in suspension cultures of Taxus spp. Cell Enzyme Microb. Technol. 32: 71–77.

    Article  CAS  Google Scholar 

  • Zimowski J. 1991. Occurrence of a glucosiltransferase specific for solanidine in potato plants. Phytochemistry 30: 1827–1831.

    Article  CAS  Google Scholar 

  • Zimowski J. 1992. Specificity and some other properties of cytosolic and membranous UDP-gluc: 3-β-hydroxysteroid glucosiltransferases from Solanum tuberosum leaves. Phytochemistry 31: 2977–2981.

    Google Scholar 

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Authors and Affiliations

  1. Faculty of Biology, Plant Biology Department (Plant Physiology), University of Murcia, 30100 Espinardo, Murcia, Spain

    P. Gómez, A. Ortuño & J.A. Del Río

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  1. P. Gómez
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  2. A. Ortuño
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  3. J.A. Del Río
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Correspondence to J.A. Del Río.

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Gómez, P., Ortuño, A. & Del Río, J. Ultrastructural changes and diosgenin content in cell suspensions of Trigonella foenum-graecum L. by ethylene treatment. Plant Growth Regulation 44, 93–99 (2004). https://doi.org/10.1023/B:GROW.0000049415.62026.c3

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  • DOI: https://doi.org/10.1023/B:GROW.0000049415.62026.c3

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