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Functional assessment of cryopreserved human aortas for pharmaceutical research

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Abstract

We evaluated the impact of standard cryopreservation on functional properties of human aortic homografts. From seven human donors, the thoracic descending aorta was obtained. Effects of cryopreservation on contractibility and endothelium function were tested. After cryopreservation no endothelium-dependent or endothelium-independent relaxation was found and the contractibility was strongly affected. Arteries showed no function and loss of endothelial integrity after cryopreservation and thawing.

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Authors and Affiliations

  1. Cryobiology Unit, Complejo Hospitalario Juan Canalejo-Teresa Herrera, 15006, La Coruña, Spain

    Ma Esther Rendal Vázquez, M. Rodríguez Cabarcos, M.V. Martínez Santos, R.O. Fernández Mallo, T. Bermúdez González & C. Andión Núñez

  2. Transplant Coordination Office, Complejo Hospitalario Juan Canalejo-Teresa Herrera, 15006, La Coruña, Spain

    J. Sánchez Ibáñez

  3. Department of Vascular Surgery, Complejo Hospitalario Juan Canalejo-Teresa Herrera, 15006, La Coruña, Spain

    R. Segura Iglesias

  4. Pathology Department, Complejo Hospitalario Juan Canalejo-Teresa Herrera, 15006, La Coruña, Spain

    G. Matheu Capó & P. Filgueira Fernández

  5. Statistics Department, Complejo Hospitalario Juan Canalejo-Teresa Herrera, 15006, La Coruña, Spain

    S. Pértega Díaz

Authors
  1. Ma Esther Rendal Vázquez
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  2. M. Rodríguez Cabarcos
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  3. M.V. Martínez Santos
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  4. R.O. Fernández Mallo
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  5. J. Sánchez Ibáñez
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  6. R. Segura Iglesias
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  7. G. Matheu Capó
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  8. P. Filgueira Fernández
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  9. S. Pértega Díaz
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  10. T. Bermúdez González
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  11. C. Andión Núñez
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Corresponding author

Correspondence to Ma Esther Rendal Vázquez.

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Rendal Vázquez, M.E., Rodríguez Cabarcos, M., Martínez Santos, M. et al. Functional assessment of cryopreserved human aortas for pharmaceutical research. Cell Tissue Banking 5, 119–123 (2004). https://doi.org/10.1023/B:CATB.0000034087.26468.5a

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  • DOI: https://doi.org/10.1023/B:CATB.0000034087.26468.5a

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