Biologia plantarum 48:491-495, 2004 | DOI: 10.1023/B:BIOP.0000047142.07987.e1
High Frequency Somatic Embryogenesis in Cotton
- 1 Research Institute for Genetic Engineering and Biotechnology, TUBITAK, Gebze-Kocaeli, Turkey
- 2 Marmara University, Goztepe, Istanbul, Turkey
- 3 Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia-Herzegovina
- 4 Faculty of Science, Istanbul University, Vezneciler, Istanbul, Turkey
A highly reproducible system for efficient somatic embryogenesis was developed to regenerate plantlets from cotton (Gossypium hirsutum L.) cultivars (Nazilli M-503 and Nazilli 143). Shoot apices, hypocotyls and nodes of 10-d-old seedlings were used as explants. High frequency (100 %) embryogenic calli was initiated from all tested explants on Murashige and Skoog (1962) (MS) media supplemented with 1 g dm-3 polyvinylpyrrolidone (PVP), 1 mg dm-3 6-benzylaminopurine (BAP), 0.5 mg dm-3 kinetin for Nazilli M-503 and 1 g dm-3 PVP, 2 mg dm-3 BAP, 0.5 mg dm-3 kinetin for Nazilli-143. Globular stage somatic embryos were produced 4 months after transfer to hormone-free MS medium supplemented with 1 g dm-3 PVP. Subsequent subculture of globular embryos every 3 weeks on hormone-free MS medium led to the development of torpedo and cotyledonary stage embryos with the frequency of 75 and 83.2 % from hypocotyl explants of Nazilli M-503 and Nazilli-143, respectively. Afterwards, mature somatic embryos were isolated and cultured on hormone-free MS medium for germination and development into plantlets. The highest germination frequency (42.9 %) for Nazilli M-503 somatic embryos were obtained on hormone-free MS medium after 5 months with hypocotyl explants, whereas germination frequencies of Nazilli-143 embryos from hypocotyl, node and apex explants varied between 22 - 30 %.
Keywords: Gossypium hirsutum L.; indirect somatic embryogenesis; hypocotyls; cotyledonary node; apex
Subjects: cotton, somatic embryology; cultivar and genotype differences, high frequency somatic embryogenesis; cytokinins; Gossypium hirsutum; in vitro culture, somatic embryogenesis; nutrient medium, Murashige and Skoog; somatic embryology
Published: December 1, 2004 Show citation
References
- Agrawal, D.C., Banerjee, A.K., Kolala, R.R., Dhage, A.B., Kulkarni, A.V., Nalwade, S.M., Hazra, S., Krishnamurthy, K.V.: In vitro induction of multiple shoots and plant regeneration in cotton (Gossypium hirsitum L.).-Plant Cell Rep. 16: 647-652, 1997. Go to original source...
- Ammirato, P.V.: Organizational events during somatic embryogenesis.-In: Green, C.E., Somers, D.A., Hackett, W., Biesboer, D.D. (ed.): Plant Tissue and Cell Culture. Pp. 57-81. Alan R. Liss, New York 1987.
- Bajrovic, K., Aydin, Y., Talas-Ogras, T., Ipekci, Z., Altinkut, A., Gencer, O., Gozukirmizi, N.: Direct plant regeneration from node and apex explants of cotton in Turkey.-Biotechnol. Biotech. Eq. 15 (2): 73-76, 2001.
- Block, R., Lankes, C.: Reasons for tissue browning of explants of the apple rootstock M9 during in vitro establishment.-Gartenbauwissenschaft 60: 276-279, 1995.
- Carman J.G.: Embryogenic cells in plant tissue cultures: occurrence and behavior.-In Vitro cell. dev. Biol. Plant 26: 746-753, 1990. Go to original source...
- Chen, Z.X., Li, S.J., Trolinder, N.L.: Some characteristics of somatic embryogenesis and plant regeneration in cotton cell suspension culture.-Sci. agr. sin. 20: 6-11, 1987.
- Cheng, G.W., Crisosto, C.H.: Browning potential, phenolic composition and polyphenooxidase activity of buffer extracts of peach and nectarine skin tissue.-J. amer. Soc. hort. Sci. 120: 835-838, 1995. Go to original source...
- Choi, Y.E., Yang, D.C., Choi, K.T.: Induction of somatic embryos by macrosalt stress from mature zygotic embryos of Panax ginseng.-Plant Cell Tissue Organ Cult. 52: 177-181, 1998. Go to original source...
- Davidonis, G.H., Hamilton, R.H.: Plant regeneration from callus tissue of Gossypium hirsutum L.-Plant Sci. Lett. 32: 89-93, 1983. Go to original source...
- D'Onofrio, C., Morini, S.: Simultaneous regeneration of different morphogenic structures from quince leaves as affected by growth regulator combination and treatment length.-Biol. Plant. 47: 321-325, 2003/4. Go to original source...
- Firoozabady, E., DeBoer, D.L.: Plant regeneration via somatic embryogenesis in many cultivars of cotton (Gossypium hirsutum L.).-In Vitro cell. dev. Biol. Plant 29: 166-173, 1993. Go to original source...
- Gamborg, O.L., Miller, R.A., Ojima, K.: Nutrient requirements of suspension cultures of soybean root cells.-Exp. Cell Res. 50: 151-158, 1968. Go to original source...
- Gawel, N.J., Robacker, C.D.: Genetic control of somatic embryogenesis in cotton petiole callus cultures.-Euphytica 49: 249-253, 1990. Go to original source...
- Gould, J., Banister, S., Hasegawa, O., Fahima, M., Smith, R.H.: Regeneration of Gossypium hirsitum and G. barbadense from shoot apex tissue for transformation.-Plant Cell Rep. 10: 12-16, 1991. Go to original source...
- Gupta, S.K., Singh, P.K., Sawant, S.V., Chaturvedi, R., Tuli, R.: Effect of light intensity on in vitro multiple shoot induction and regeneration of cotton (Gossypium hirsutum L. cv. Khandawa-2).-Indian J. exp. Bot. 38: 399-401, 2000.
- Gupta, S.K., Srivastava, A.K., Singh, P.K., Tuli, R.: In vitro proliferation of shoots from cotyledonary node of cotton.-Plant Cell Tissue Organ Cult. 51:149-152, 1997. Go to original source...
- Hemphill, J.K., Maier, C.G.A., Chapman, K.D.: Rapid in vitro plant regeneration of cotton (Gossypium hirsitum L.). Plant Cell Rep. 17: 273-278, 1998. Go to original source...
- Korban, S.S., O'Connor, P.A., Elobeidy, A.: Effect of thidiazuron, naphthalene acetic acid, dark incubation and genotype on shoot organogenesis from Malus leaves.-J. hort Sci. 67: 341-349, 1992. Go to original source...
- Kumar, S., Pental, D.: Regeneration of Indian cotton variety MCU-5 through somatic embryogenesis.-Curr. Sci. 74: 538-540, 1998.
- Kumria, R., Sunnichan, V.G., Das, D.K., Gupta, S.K., Reddy, V.S., Bhatnagar, R.K., Leelavathi, S.: High-frequency somatic embryo production and maturation into normal plants in cotton (Gossypium hirsutum) through metabolic stress.-Plant Cell Rep. 21: 635-639, 2003. Go to original source...
- McKersie, B.D., Brown, D.C.W.: Somatic embryogenesis and artificial seeds in forage legumes.-Seed Sci. Res. 6: 109-126, 1996. Go to original source...
- Mishra, R., Wang, H.Y., Yadav, N.R., Wilkins, T.A.: Development of a highly regenerable elite Acala cotton (Gossypium hirsutum cv. Maxa)-a step towards genotype-independent regeneration.-Plant Cell Tissue Organ Cult. 73: 21-35, 2003. Go to original source...
- Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 80: 662-668, 1962. Go to original source...
- Ndoumou, D.O., Ndzomo, G.T., Niemenak, N.: Phenol content, acidic peroxidase and IAA-oxidase during somatic embryogenesis in Theobroma cacao L.-Biol. Plant. 39: 337-347, 1997. Go to original source...
- Ogburia, M.N.: Somatic embryogenesis, plantlet regeneration and micropropagation of cultivars and F1 hybrids of Manihot esculenta.-Biol. Plant. 47: 429-432, 2003/4. Go to original source...
- Predeiri, S., Fasola, F., Malavasi, F.: High frequency shoot regeneration from leaves of apple root stock M26 (Malus punila).-Plant Cell Tissue Organ Cult. 17: 133-142, 1989. Go to original source...
- Price, H.J., Smith, R.H.: Somatic embryogenesis in suspension cultures of Gossypium klotzschianum Anderss.-Planta 145: 305-307, 1979. Go to original source...
- Rajasekaran, K., Grula, J.W., Hudspeth, R.L., Pofelis, S., Anderson, D.M.: Herbicide-resistant Acala and Coker cotton transformed with a native gene encoding mutant forms of acetohydroxy acid synthase.-Mol. Breed. 2: 307-317, 1996. Go to original source...
- Trolinder, N.L., Chen, X.X.: Genotype specificity of the somatic embryogenesis in cotton.-Plant Cell Rep. 8: 133-136, 1989. Go to original source...
- Trolinder, N.L., Goodin, J.R.: Somatic embryogenesis and plant regeneration in Gossypium hirsutum L.-Plant Cell Rep. 6: 231-234, 1987. Go to original source...
- Voo, K.S., Rugh, C.L., Kamalay, J.C.: Indirect somatic embryogenesis and plant recovery from cotton (Gossypium hirsutum L.).-In Vitro cell. dev. Biol. Plant 27: 117-124, 1991. Go to original source...
- Zhang, B.H.: A rapid induction method for cotton somatic embryos.-Chin. Sci. Bull. 39: 1340-1342, 1994. Go to original source...
- Zhang, B.H., Feng, R.: List of cotton tissue culture.-Plant Physiol. Commun. 28: 308-314, 1992.
- Zhang, B.H., Feng, R., Liu, F., Wang, Q.: High frequency somatic embryogenesis and plant regeneration of an elite Chinese cotton variety.-Bot. Bull. Acad. sin. 42: 9-16, 2001.
- Zhang, B.H., Feng, R., Liu, F., Yao, C.B.: Direct induction of cotton somatic embryogenesis.-Chin. Sci. Bull. 44: 766-767, 1999. Go to original source...
- Zhang, B.H., Li, X.L., Li, F.L., Li, F.G.: Plant recovery from cotton somatic embryos.-Acta agr. Boreali-occidentalis sin. 2 (4): 24-28, 1993.
- Zhang, B.H., Liu, F., Yak, C.B.: Plant regeneration via somatic embryogenesis in cotton.-Plant Cell Tissue Organ Cult. 60: 89-94, 2000. Go to original source...
- Zhang, B.H., Zhao, B.S.: Cotton biotechnology and its application.-China Agricultural Press, Beijing 1997.