biologia plantarum

Biologia plantarum 48:129-132, 2004 | DOI: 10.1023/B:BIOP.0000024289.68669.ef

In vitro Plant Regeneration and Genotype Conservation of Eight Wild Species of Curcuma

R.K. Tyagi^1,*, A. Yusuf², P. Dua¹, A. Agrawal¹

¹ Tissue Culture and Cryopreservation Unit, National Bureau of Plant Genetic Resources, New Delhi, India

² Albert Katz Center for Desert Agrobiology, Ben-Gurion University of the Negev, Sede Boquer Campus, Israel

In vitro protocols for plantlet regeneration and medium-term genotype conservation of eight wild species of Curcuma have been optimized. Both the phenomena were genotype-dependent and influenced significantly by type and concentration of cytokinins used. In general, benzyladenine (BA) was found superior to other cytokinins tested for plantlet regeneration and γ,γ-dimethylallylaminopurine (2iP) for conservation. Number of shoots per culture ranged from 1.3 to 7.2 and conservation period from 264 to 379 d. In 30-d-old cultures, highest frequency of shoot regeneration could be obtained in C. malabarica (7.2 shoots per culture) on MS + 11.4 μM zeatin. Curcuma sp. (unidentified wild species) could be conserved for maximum period (379 d) on MS + 24.6 μM 2iP followed by C. aromatica (363 d) on MS + 22.8 μM zeatin. The tissue culture-raised plantlets were morphologically similar to their parents. The in vitro-conserved plants multiplied rapidly in tissue cultures and produced normal rhizomes upon transfer to soil in net house.

Keywords: germplasm; genetic resources; micropropagation; tissue culture

Subjects: Curcuma aeruginosa; Curcuma aromatica; Curcuma brog; Curcuma caesia; Curcuma malabarica; Curcuma ractatanta; Curcuma soloensis; cytokinins; genetic resources; genotype conservation; germplasm; in vitro culture, micropropagation; in vitro culture, regeneration, organogenesis; in vitro culture, root, rooting; nutrient medium, Murashige and Skoog; turmeric, genotype conservation

Published: March 1, 2004  Show citation