Abstract
A continuous supply of O2 is important for itaconic acid production in Aspergillus terreus. Any interruption of aeration significantly reduces itaconic acid production. To overcome this effect, A. terreus M8 was transformed with the Vitreoscilla hemoglobin gene (vgb) which, as shown by Southern hybridization, was integrated into the recipient chromosome. The activity of the expressed hemoglobin was confirmed by a CO-difference spectrum. During itaconic acid production, the effect of a break in aeration during cultivation in the transformant with the vgb gene is alleviated. Additionally, the transformant shows improved itaconic acid production.
Similar content being viewed by others
References
DeModena JA, Gutiérrez S, Velasco J, Fernández FJ, Fachini RA, Galazzo JL, Hughes DE, Martín JF (1993) The production of cephalosporin C by Acremonium chrysogenum is improved by the intracellular expression of a bacterial hemoglobin. Bio/Technology 11: 926–929.
Fincham JR (1989) Transformation in fungi. Microbiol. Rev. 53: 148–170.
Frey AD, Fiaux J, Szyperski T, Wuthrich K, Bailey JE, Kallio PT (2001) Dissection of central carbon metabolism of hemoglobin-expressing Escherichia coli by 13C nuclear magnetic resonance flux distribution analysis in microaerobic bioprocesses. Appl. Environ. Microbiol. 67: 680–687.
Geckil H, Stark BC, Webster DA (2001) Cell growth and oxygen uptake of Escherichia coli and Pseudomonas aeruginosa are differently effected by the genetically engineered Vitreoscilla hemoglobin gene. J. Biotechnol. 85: 57–66.
Gyamerah MH (1995) Oxygen requirement and energy relations of itaconic acid fermentation by Aspergillus terreus NRRL 1960. Appl. Microbiol. Biotechnol. 44: 20–26.
Khosla C, Bailey JE (1988) Heterologous expression of a bacterial hemoglobin improves the growth properties of recombinant Escherichia coli. Nature 331: 633–635.
Khosravi M, Webster DA, Stark BC (1990) Presence of the bacterial hemoglobin gene improves α-amylase production of a recombinant Escherichia coli strain. Plasmid 24: 190–194.
Minas W, Brunker P, Kallio PT, Bailey JE (1998) Improved erythromycin production in a genetically engineered industrial strain of Saccharopolyspora erythraea. Biotechnol. Prog. 14: 561–566.
Punt J, Oliver RP, Dingemanse MA, Pouwels PH, van den Hondel CA (1987) Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene 56: 117–124.
Simpson IN, Caten CE (1979) Induced quantitative variation for penicillin titre in clonal populations of Aspergillus nidulans. J. Gen. Microbiol. 110: 1–12.
Tilburn J, Scazzocchio C, Taylor GG, Zabicky-Zissman JH, Lockington RA, Davies RW (1983) Transformation by integration in Aspergillus nidulans. Gene 26: 205–221.
Ventura L, Ramón D (1991) Transformation of Aspergillus terreus with the hygromycin B resistance marker from Escherichia coli. FEMS Microbiol. Lett. 66: 345–350.
Webster DA, Liu CY (1974) Reduced nicotinamide adenine dinucleotide cytochrome o reductase associated with cytochrome o purified from Vitreoscilla. J. Biol. Chem. 249: 42–59.
Wei ML, Webster DA, Stark BC (1998) Genetic engineering of Serratia marcescens with bacterial hemoglobin gene: effects on growth, oxygen utilization, and cell size. Biotechnol. Bioeng. 57: 477–483.
Wilke T, Vorlop KD (2001) Biotechnological production of itaconic acid. Appl. Microbiol. Biotechnol. 56: 289–295.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lin, YH., Li, YF., Huang, MC. et al. Intracellular expression of Vitreoscilla hemoglobin in Aspergillus terreus to alleviate the effect of a short break in aeration during culture. Biotechnology Letters 26, 1067–1072 (2004). https://doi.org/10.1023/B:BILE.0000032964.15178.7c
Issue Date:
DOI: https://doi.org/10.1023/B:BILE.0000032964.15178.7c