Abstract
To investigate the phylogenetic relationships of living marsupials, morphometric and G-banded chromosome analyses were made in the Chilean species Dromiciops gliroides (Microbiotheria) and Thylamys elegans (Didelphimorphia). Chromosome arm lengths and patterns of G-bands were compared in at least eight bone marrow metaphase spreads in six and nine specimens, respectively. They were contrasted with those published for another 11 American and Australian genera. Three of six autosomal pairs (A1, A3, and C2) were uniquely shared by Dromiciops and some Australian species, being different in shape and G-banded patterns from those with similar total sizes in Thylamys and other South American didelphoid karyotypes. Such chromosomal correspondences suggest the past occurrence of at least three pericentric inversions. A table of character states constructed from chromosomal G-band comparisons is presented, showing that cytogenetic data agree with Szalay's (1982) hypothesis on the affinities of the South American Dromiciops with Australian marsupials.
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LITERATURE CITED
Carlini, A. A., Pascual, R., Reguero, M. A., Scillato-Yane, G. H., Tonni, E. P., and Vizcaino, S. F. (1990). The first Paleogene land placental mammals from Antarctica: Its paleoclimatic and paleobiogeographical bearing. Abstracts, Fourth International Congress of Systematic and Evolutionary Biology, University of Maryland, College Park.
Chiarelli, B. A., Sarti-Chiarelli, M., and Shafer, D. A. (1972). Chromosome banding with trypsin. Mamm. Chrom. Newslett. 13: 44–45.
Crisci, J., Cigliano, M. M., Morrone, J. J., and Roig-Juñent, S. (1991). Historical biogeography of southern South America. Syst. Zool. 40: 152–171.
Crossen, P. E. (1972). The Crossen procedure. Mamm. Chrom. Newslett. 13: 40.
Dyzenchauz, F. J., Barros, M. A., Kirsch, J. A. W., and Reig, O. A. (1993). Dromiciops australis, G-banding analysis reinforce its close association to Australian marsupials. In: Abstracts, VI International Theriological Congress, Sydney, Australia, p. 82.
Gallardo, M. H. and Patterson, B. D. (1987). An additional 14-chromosome karyotype and sex-chromosome mosaicism in South American marsupials. In: Studies in Neotropical Mammalogy: Essays in Honor of Philip Hershkovitz, B. D. Patterson and R. L. Timm, eds., Fieldiana Zool. 39: 111–116.
Gardner, A. L. (1993). Order Didelphimorphia. In: Mammal Species of the World: A Taxonomic and Geographic Reference, D. E. Wilson and D. M. Reeder, eds., pp. 15–23, Smithsonian Institution Press, Washington, DC.
Hayman, D. L., and Martin, P. G. (1974). Mammalia I: Monotremata and Marsupialia, Vol. 4: Chordata 4. In: Animal Cytogenetics, B. John, ed., Gebrüder Borntraeger, Berlin and Stuttgart.
Hayman, D. L., Moore, H. D. M., and Evans, E. P. (1988). Further evidence of novel sex differences in chiasma distribution in marsupials. Heredity 61: 455–458.
Hershkovitz, P. (1992). Ankle bones: The Chilean opossum Dromiciops gliroides Thomas, and marsupial phylogeny. Bonn. Zool. Beit. 43: 181–213.
Kirsch, J. A. W., Dickerman, A. W., Reig, O. A., and Springer, M. S. (1991). DNA hybridization evidence for the Australian affinity of the American marsupial. Dromiciops australis. Proc. Natl. Acad. Sc. USA 88: 10465–10469.
Lee, M. R., and Elder, F. F. B. (1980). Yeast stimulation of bone marrow mitoses for cytogenetic investigations. Cytogenet. Cell Genet. 26: 36–40.
Levan, A., Fredga, K., and Sandberg, A. (1964). Nomenclature for centromeric position on chromosomes. Hereditas 52: 201–220.
Luckett, W. P. (1994). Suprafamilial relationships within Marsupialia: Resolution and discordance from multidisciplinary data. J. Mammal. Evol. 2: 255–283.
Marshall, L. G. (1978). Dromiciops australis. Mammal. Species 99: 1–5 (American Society of Mammalogists).
Palma, R. E. (1997). Thylamys elegans. Mammal. Species 572: 1–4 (American Society of Mammalogists).
Palma, R. E., and Yates, T. L. (1997). Phylogeny of southern South American mouse opossums (Thylamys, Didelphidae) based on allozyme and chromosomal data. Z. Säugetierk. 62: 1–15.
Pascual, R., Archer, M., Ortiz, J. E., Prado, J. L., Godthelp, H. and Hand, S. J. (1992). First discovery of monotremes in South America. Nature 356: 704–706.
Reig, O. A., Fernández-Donoso, R., and Spotorno, A. (1972). Further occurrence of a 2N = 14 chromosomes karyotype in two species of Chilean didelphoid marsupials. Z. Säugetierk. 37: 37–42.
Reig, O. A., Gardner, A. L., Bianchi, N. O., and Patton, J. L. (1977). The chromosomes of the Didelphidae (Marsupialia) and their evolutionary significance. Biol. J. Linn. Soc. 9: 191–216.
Retief, J. D., Krajewski, C., Westerman, M., Winkfein, R. J., and Dixon, G. H. (1995). Molecular phylogeny and evolution of marsupial protamine P1 genes. Proc. Roy. Soc. London B 259: 7–14.
Rofe, R., and Hayman, D. (1985). G-banding evidence for a conserved complement in the Marsupialia. Cytogenet. Cell Genet. 39: 40–50.
Seluja, G. A., Di Tomaso, M. V., Brum-Zorrilla, N., and Cardoso, H. (1984). Low karyotypic variation in two Didelphis (Marsupialia): Karyogram and chromosome banding analysis. J. Mammal. 65: 702–704.
Sharman, G. B. (1972). The chromosomes of non-eutherian mammals. In: Cytotaxonomy and Vertebrate Evolution, A. B. Chiarelli and E. Capanna, eds., pp. 485–530, Academic Press, London, New York.
Sharman, G. B. (1982). Karyotypic similarities between Dromiciops australis (Microbiotheriidae, Marsupialia) and some Australian marsupials. In: Carnivorous Marsupials, M. Archer, ed., pp. 711–714, Royal Society of New South Wales, Sydney, Australia.
Spotorno, A., and Fernández-Donoso, R. (1970). The chromosomes of the “monito del monte,” Dromiciops australis Phil. Mamm. Chrom. Newslett. 12: 40–41.
Spotorno, A., Brum, N., and Di Tomaso, M. (1987). Comparative cytogenetics of South American deer. Fieldiana Zool. 84: 473–483.
Springer, M. S., Westerman, M., and Kirsch, J. A. W. (1994). Relationships among orders and families of marsupials based on 12S ribosomal DNA sequences and the timing of the marsupial radiation. J. Mammal. Evol. 2: 85–115.
Sumner, A. T. (1972). A simple technique for demonstrating centromeric heterochromatin. Exp. Cell Res. 75: 304–306.
Szalay, F. S. (1982). A new appraisal of marsupial phylogeny and classification. In: Carnivorous Marsupials, M. Archer, ed., pp. 621–640, Royal Zoological Society, New South Wales, Sydney.
Temple-Smith, P. (1987). Sperm ultrastructure and marsupial phylogeny. In: Possums and Opossums: Studies in Evolution, M. Archer, ed., pp. 171–193, Surrey Beatty & Sons, Chipping Norton.
Woodburne, M. O., and Case, J. A. (1996). Dispersal, vicariance, and the late Cretaceous to early Tertiary land mammal biogeography from South America to Australia. J. Mammal. Evol. 3: 121–161.
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Spotorno, A.E., Marin, J.C., Yévenes, M. et al. Chromosome Divergences Among American Marsupials and the Australian Affinities of the American Dromiciops . Journal of Mammalian Evolution 4, 259–269 (1997). https://doi.org/10.1023/A:1027374514503
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DOI: https://doi.org/10.1023/A:1027374514503