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The Yeast Two-hybrid System and Its Pharmaceutical Significance

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Abstract

The detected phenotypes in many diseases are caused from dysfunction in protein-protein, protein-DNA and receptor-ligand interactions. Therefore, determination of these molecular interactions followed by designing or screening the compounds to target these interactions provides a significant challenge in drug development. This review aims to highlight the yeast two-hybrid system in determination of protein-protein interactions and its possible outcomes in pharmaceutical research. The variations of the basic methodology as one- and three-hybrid systems are also disussed in relation to their potential pharmaceutical applications.

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Authors and Affiliations

  1. Department of Pharmaceutical Biotechnology, School of Pharmacy, Ege University, Izmir, 35100, Turkey

    Zeki Topcu

  2. Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York, 10029

    Katherine L. B. Borden

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  1. Zeki Topcu
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  2. Katherine L. B. Borden
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Topcu, Z., Borden, K.L.B. The Yeast Two-hybrid System and Its Pharmaceutical Significance. Pharm Res 17, 1049–1055 (2000). https://doi.org/10.1023/A:1026493310144

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