Skip to main content
SpringerLink
Log in

Analysis of Combined Action of Antagonists of Excitatory and Inhibitory Amino Acids on Motoneuron Postsynaptic Potentials of the Frog Rana ridibunda

  • Published:
Journal of Evolutionary Biochemistry and Physiology Aims and scope Submit manuscript

Abstract

The effect of blockers of excitatory and inhibitory amino acid receptors on postsynaptic potentials (PSP) evoked by activation of three synaptic inputs of the lumbar motoneuron (stimulation of the dorsal root, reticular formation, ventral and lateral columns) was studied on preparation of the isolated spinal cord of the frog Rana ridibunda. It has been shown that sensitivity of PSP to antagonists differs in different motoneurons, in the same motoneuron at activation of different inputs, and in the same input in different PSP components. It has been found that many descendent (DC) PSPs resistant to kynurenate or CNQX [1] were inhibited by blockers of inhibitory receptors. In this case the early component of DC-PSP varied considerably by amplitude and changed its polarity from positive to negative on the background of a low transmembrane depolarizing current. These changes were absent under conditions of replacement of chlorine ion by sulfate in the perfusion solution or treatment of the spinal cord with a blocker of inhibitory amino acids. All this allows suggesting that these DC-PSPs or their components were inhibitory. A part of PSPs resistant to kynurenate and CNQX were also resistant to the blockers of inhibitory amino acids (strychnine, picrotoxin, and bicuculline). In some cases, as a result of treatment with convulsants, the same blockers of excitatory receptors inhibited the initially resistant PSPs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Kurchavyi, G.G., Kalinina, N.I., and Vesselkin, N.P., Different Sensitivity of Synaptic Inputs of Frog Motoneurons to Antagonists of Excitatory Amino Acids, Zh. Evol. Biokhim. Fiziol., 2003, vol. 39, pp. 144–153.

    Google Scholar 

  2. Kalinina, N.I., Kurchavyi, G.G., Vesselkin, N.P., Antonov, S.M., and Magazanik, L.G., Receptors in Different Excitatory Inputs of the Frog Motoneuron, Biol. Membrany, 1992, vol. 9, pp. 1117–1119.

    Google Scholar 

  3. Kurchavyi, G.G., Suderevskaya, E.I., and Vesselkin, N.P., Effects of Antagonists of Excitatory Amino Acids on Postsynaptic Potentials in Motoneurons of the Frog, Rana ridibunda, Zh. Evol. Biokhim. Fiziol., 1995, vol. 31, pp. 562–571.

    Google Scholar 

  4. Shapovalov, A.I. and Shiriaev, B.I., Reticulo-Spinal and Propriospinal Monosynaptic Influences on Frog Motoneurons, Neirofiziologiya, 1973, vol. 5, pp. 164–173.

    Google Scholar 

  5. Kalinina, N.I., Kurchavyi, G.G., and Ryabov, B.T., Equilibrium Potential of Monosynaptic EPSPs in Frog Motoneurons, Neirofiziologiya, 1986, vol. 18, pp. 534–542.

    Google Scholar 

  6. Jahr, C.E. and Yoshioka, K., Ia Afferent Excitation of Motoneurones in the in vitro New-Born Rat Spinal Cord Is Selectively Antagonized by Kynurenate, J. Physiol., 1986, vol. 370, pp. 515–530.

    Google Scholar 

  7. Katz, B. and Miledi, R., A Study of Spontaneous Miniature Potentials in Spinal Motoneurones, J. Physiol., 1963, vol. 168, pp. 389–422.

    Google Scholar 

  8. Bührle, Ch.Ph. and Sonnhof, U., The Ionic Mechanism of Postsynaptic Inhibition in Motoneurones of the Frog Spinal Cord, Neurosci., 1985, vol. 14, no. 2, pp. 581–592.

    Google Scholar 

  9. Fulton, B.P., Miledi, R., and Takahashi, T., Electrical Synapses between Motoneurones in the Spinal Cord of the Newborn Rat, Proceed. Royal Soc. B, 1980, vol. 208, pp. 115–120.

    Google Scholar 

  10. Eccles, J., Fiziologiya nervnoi kletki (Physiology of the Nerve Cell), Moscow, 1959.

  11. Cruce, W.L.R., A Supraspinal Monosynaptic Input to Hindlimb Motoneurons in Lumbar Spinal Cord of the Frog, Rana catesbeiana, J. Neurophysiol., 1974, vol. 37, pp. 691–704.

    Google Scholar 

  12. Fadiga, E. and Brookhart, J.M., Monosynaptic Activation of Different Portions of the Motor Neuron Membrane, Amer. J. Physiol., 1960, vol. 198, pp. 693–703.

    Google Scholar 

  13. Richens, A., The Action of General Anesthetic Agents on Root Responses of the Frog Isolated Spinal Cord, Brit. J. Pharmacol., 1969, vol. 36, pp. 294–311.

    Google Scholar 

  14. Brookhart, J.M., Machne, X., and Fadiga, E., Patterns of Motor Neuron Discharge in the Frog, Arch. Ital. Biol., 1959, vol. 97, pp. 53–67.

    Google Scholar 

  15. Davies, C.H., Davies, S.N., and Collingridge, G.L., Paired-Pulse Depression of Monosynaptic GABAMediated Inhibitory Postsynaptic Responses in Rat Hippocampus, J. Physiol., 1990, vol. 424, pp. 513–531.

    Google Scholar 

  16. Kostyuk, P.G., Struktura i funktsiya niskhodyashchikh sistem spinnogo mozga (Structure and Function of the Spinal Cord Descending Systems), Leningrad, 1973.

  17. Wilson, V.J., Yoshida, M., and Schor, P.H., Monosynaptic Excitation and Inhibition of Thoracic Motoneurons by Fibers in the Medial Longitudinal Fasciculus, Brain Res., 1970, vol. 18, pp. 181–184.

    Google Scholar 

  18. Wilson, V.J., Yoshida, M., and Schor, P.H., Supraspinal Monosynaptic Excitation and Inhibition of Thoracic Back Motoneurons, Exp. Brain Res., 1970, vol. 11, pp. 282–295.

    Google Scholar 

  19. Simpson, J.I., Functional Synaptology of the Spinal Cord, Frog Neurobiology, Llinas, R. and Precht, W., Eds., Berlin; Heidelberg: Springer, 1976, pp. 728–749.

    Google Scholar 

  20. Tamarova, Z.A., Shapovalov, A.I., and Shiriaev, B.I., Synaptic Effects in Terminals of Individual Primary Afferent Fibers Connected Mono-and Polysynaptically with Spinal Cord Motoneurons, Fiziol. Zh. SSSR, 1981, vol. 67, pp. 1511–1520.

    Google Scholar 

  21. Barker, J.L. and Nicoll, R.A., The Pharmacology and Ionic Dependency of Amino Acid Responses in the Frog Spinal Cord, J. Physiol., 1973, vol. 228, pp. 259–277.

    Google Scholar 

  22. Gonzalez, H., Jimenez, I., and Rudomin, P., Bulbospinal Inhibition of PAD Elicited by Stimulation of Afferent and Motor Axons in the Isolated Frog Spinal Cord and Brainstem, Exp. Brain Res., 1992, vol. 88, pp. 106–116.

    Google Scholar 

  23. Brookhart, J.M. and Kubota, K., Studies of the Integrative Function of the Motor Neurone, Progress in Brain Research. Brain Mechanisms, Moruzzi, G., Fessard, A., and Jasper, H.H., Eds., New York: Elsevier, 1963, vol. 1, pp. 38–61.

    Google Scholar 

  24. Eccles, J., Fiziologiya sinapsov (Physiology of Synapses), Moscow, 1964.

  25. McGeer, P.L., Eccles, J., and McGeer, E.G., Molecular Neurobiology of the Mammalian Brain, Plenum, New York and London, 1987.

    Google Scholar 

  26. Kubota, K. and Brookhart, J.M., Inhibitory Synaptic Potential of Frog Motor Neurons, Amer. J. Physiol., 1963, vol. 204, pp. 660–666.

    Google Scholar 

  27. Kurchavyi, G.G., Karamian, O.A., Kalinina, N.I., and Vesselkin, N.P., On the Mechanism of Penicillin Effect on Activity of Motoneurons in the Frog, Rana ridibunda, Zh. Evol. Biokhim. Fiziol., 1997, vol. 33, pp. 157–168.

    Google Scholar 

  28. Davies, J. and Watkins, J.C., Role of Excitatory Amino Acid Receptors in Mono-and Polysynaptic Excitation in the Cat Spinal Cord, Exp. Brain Res., 1983, vol. 49, pp. 280–290.

    Google Scholar 

  29. Headley, P.M. and Grillner, S., Excitatory Amino Acids and Synaptic Transmission: the Evidence for a Physiological Function, TiPS, 1990, vol. 11, pp. 205–211.

    Google Scholar 

  30. Foster, A.C. and Fagg, J.E., Acidic Amino Acid Binding Sites in Mammalian Neuronal Membranes: Their Characteristics and Relationship to Synaptic Receptors, Brain Res. Rev., 1984, vol. 7, pp. 103–164.

    Google Scholar 

  31. Monaghan, D.T., Bridges, R.J., and Cotman, C.W., The Excitatory Amino Acid Receptors: Their Classes, Pharmacology and Distinct Properties in the Function of the Central Nervous System, Annu. Rev. Pharmacol. Toxicol., 1989, vol. 29, pp. 365–402.

    Google Scholar 

  32. Lodge, D., Hather, N.Y., Jones, M.G., Palmer, A.J., Fleatcher, G., Zeman, S., Lam, K., Yuliang, S., and Qiang, W., Electropharmacological Evidence for Glutamate Receptor Subtypes, Excitatory Amino Acids, Meldrum, B.S., Moroni, F., Simon, R.P., and Woods, J.H., Eds., New York: Raven, 1991, pp. 163–175.

    Google Scholar 

  33. Mayer, M.L. and Westbrook, G.L., The Physiology of Excitatory Amino Acids in the Vertebrate Central Nervous System, Progr. Neurobiol., 1987, vol. 28, pp. 197–276.

    Google Scholar 

  34. Holohean, A.M., Hackman, J.C., and Davidoff, R.A., Mechanisms Involved in the Metabotropic Glutamate Receptor-Enhancement of NMDA-Mediated Motoneurone Responses in Frog Spinal Cord, Br. J. Pharmac., 1999, vol. 126, pp. 333–341.

    Google Scholar 

  35. Walsh, J.P., Hull, C.D., Levine, M.S., and Buchwald, N.A., Kynurenic Acid Antagonizes the Excitatory Postsynaptic Potential Elicited in Neostriatal Neurons in the in vitro Slice of the Rat, Brain Res., 1989, vol. 480, pp. 290–293.

    Google Scholar 

  36. Yoshimura, M. and Jessell, T., Amino Acid-Mediated EPSPs at Primary Afferent Synapses with Substantia Gelatinosa Neurons in the Rat Spinal Cord, J. Physiol., 1990, vol. 430, pp. 315–335.

    Google Scholar 

  37. Schneider, S.P. and Perl, E.R., Comparison of Primary Afferent and Glutamate Excitation of Neurones in the Mammalian Spinal Dorsal Horn, J. Neurosci., 1988, vol. 8, pp. 2062–2073.

    Google Scholar 

  38. Honore, T., Sheardown, M.J., Nielsen, E.B., Judge, M.E., and Jakobsen, P., Quinoxalinediones non-N-methyl-D-aspartate Receptor Antagonists as Potential Drug Candidates, Excitatory Amino Acids, Meldrum, B.S., Moroni, F., Simon, R.P., and Woods, J.H., Eds., New York: Raven, 1991, pp. 451–460.

    Google Scholar 

  39. Evans, R.H., Evans, S.J., Pook, P.C., and Sunter, D.C., A Comparison of Excitatory Amino Acid Antagonists Acting at Primary Afferent C Fibres and Motoneurones of the Isolated Spinal Cord of the Rat, Br. J. Pharmac., 1987, vol. 91, pp. 531–537.

    Google Scholar 

  40. Thomson, A.M., Glycine Modulation of the NMDA Receptor/Channel Complex, TiNS, 1989, vol. 12, no. 9, pp. 349–353.

    Google Scholar 

  41. Evans, R.H., Francis, A.A., Hunt, K., Oakes, D.J., and Watkins, J.C., Antagonism of Excitatory Amino Acid-Induced and of Synaptic Excitation in the Isolated Spinal Cord of the Frog, Br. J. Pharmacol., 1979, vol. 67, pp. 591–603.

    Google Scholar 

  42. Long, S.K., Smith, D.A.S., Siarey, R.J., and Evans, R.H., Effect of 6-Cyano-2,3-dihydroxy-7-nitro-quinoxaline (CNQX) on Dorsal Root-, NMDA-, Kainate-and Quisqualate-Mediated Depolarization of Rat Motoneurones in vitro, Br. J. Pharmacol., 1990, vol. 100, pp. 850–854.

    Google Scholar 

  43. Collingridge, G.L. and Lester, R.A.J., Excitatory Amino Acid Receptors in the Vertebrate Central Nervous System, Pharmacol. Rev., 1989, vol. 40, pp. 143–210.

    Google Scholar 

  44. Stone, T.W., The Endogenous Antagonist Kynurenic Acid. Effects at Kainate Receptors, Excitatory Amino Acids, Meldrum, B.S., Moroni, F., Simon, R.P., and Woods, J.H., Eds., New York: Raven, 1991.

    Google Scholar 

  45. Perkins, M.N. and Stone, T.W., An Iontophoretic Investigation of the Actions of Convulsant Kynurenines and Their Interaction with the Endogenous Excitant Quinolinic Acid, Brain Res., 1982, vol. 247, pp. 184–187.

    Google Scholar 

  46. Ault, B., Evans, R.H., Francis, A.A., Oakes, D.J., and Watkins, J.C., Selective Depression of Excitatory Amino Acid Induced Depolarizations by Magnesium Ions in Isolated Spinal Cord Preparations, J. Physiol., 1980, vol. 307, pp. 413–428.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia

    G. G. Kurchavyi, N. I. Kalinina & N. P. Vesselkin

Authors
  1. G. G. Kurchavyi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. I. Kalinina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. P. Vesselkin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kurchavyi, G.G., Kalinina, N.I. & Vesselkin, N.P. Analysis of Combined Action of Antagonists of Excitatory and Inhibitory Amino Acids on Motoneuron Postsynaptic Potentials of the Frog Rana ridibunda . Journal of Evolutionary Biochemistry and Physiology 39, 321–332 (2003). https://doi.org/10.1023/A:1026199909712

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1026199909712

Keywords

Search

Navigation