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Determination of Glass Transition Temperature and in Situ Study of the Plasticizing Effect of Water by Inverse Gas Chromatography

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Abstract

Purpose. To use an inverse gas chromatographic (IGC) method to determine the glass transition temperature (Tg) of some amorphous pharmaceuticals and to extend this technique for the in situ study of the plasticizing effect of water on these materials.

Methods. Amorphous sucrose and colyophilized sucrose-PVP mixtures were the model compounds. Both IGC and differential scanning calorimetry (DSC) were used to determine their Tg. By controlling the water vapor pressure in the IGC sample column, it was possible to determine the Tg of plasticized amorphous phases. Under identical temperatures and vapor pressures, the water uptake was independently quantified in an automated water sorption apparatus.

Results. The Tg of the dry phases, determined by IGC and by DSC, were in very good agreement. With an increase in the environmental relative humidity (RH), there was a progressive decrease in Tg as a result of the plasticizing effect of water. Because the water uptake was independently quantified, it was possible to use the Gordon-Taylor equation to predict the Tg values of the plasticized materials. The predicted values were in very good agreement with those determined experimentally using IGC. A unique advantage of this technique is that it provides complete control over the sample environment and is thus ideally suited for the characterization of highly reactive amorphous phases.

Conclusions. An IGC method was used (a) to determine the glass transition temperature of amorphous pharmaceuticals and (b) to quantify the plasticizing effect of water on multicomponent systems.

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Authors and Affiliations

  1. University of Minnesota, Minneapolis, Minnesota, 55455

    Rahul Surana, Abira Pyne & Raj Suryanarayanan

  2. 3M Drug Delivery Systems Division, 3M Center, St. Paul, Minnesota, 55144

    Rahul Surana

  3. Aventis Pharmaceuticals, Holmes Chapel, Cheshire, UK

    Linda Randall & N. Murti Vemuri

  4. Eli Lilly & Company, Lilly Corporate Center, Indianapolis, Indiana, 46285

    Abira Pyne

Authors
  1. Rahul Surana
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  2. Linda Randall
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  3. Abira Pyne
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  4. N. Murti Vemuri
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  5. Raj Suryanarayanan
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Correspondence to N. Murti Vemuri.

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Surana, R., Randall, L., Pyne, A. et al. Determination of Glass Transition Temperature and in Situ Study of the Plasticizing Effect of Water by Inverse Gas Chromatography. Pharm Res 20, 1647–1654 (2003). https://doi.org/10.1023/A:1026199604374

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