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Biologically Active Polypeptides and Hydrolytic Enzymes in Sea Anemones of Northern Temperate Waters

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Abstract

The content of biologically active polypeptides in aqueous and ethanol extracts of seven sea anemone species collected near Sakhalin Island (Sea of Okhotsk) and in Posyet Bay (Sea of Japan) was analyzed. Water extracts of the sea anemone Cribrinopsis similis showed the highest hemolytic activity, while ethanol extracts proved to have toxic properties. The levels of toxic and hemolytic activity of extracts of sea anemones inhabiting northern temperate waters were 2 to 3 orders of magnitude lower, compared to tropic species. The reason for this is likely to be the differences in the habitat conditions and biological traits of these animals. The water extracts of all species possessed proteolytic, phospholipase A2, and low DNAase activities, except Actinostola sp., whose aqueous extract contained a high activity alkaline DNAase. The species studied contained a wide range of proteinase inhibitors, O-glycosyl hydrolases (glycosidases and polysaccharide hydrolases). Water extracts of C. similis and Stomphia coccinea possessed the highest laminarinase activity. High activity of N-galactopyranosidase was found in water extracts of S. coccinea and Oulactis orientalis.

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Authors and Affiliations

  1. Pacific Institute of Bioorganic Chemistry, Far East Division, Russian Academy of Sciences, Vladivostok, 690022, Russia

    E. V. Klyshko, A. P. Il'ina, M. M. Monastyrnaya, Yu. V. Burtseva, T. A. Zykova, N. I. Menzorova & E. P. Kozlovskaya

  2. Institute of Marine Biology, Far East Division, Russian Academy of Sciences, Vladivostok, 690041, Russia

    E. E. Kostina

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  1. E. V. Klyshko
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  2. A. P. Il'ina
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  3. M. M. Monastyrnaya
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  8. E. P. Kozlovskaya
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Klyshko, E.V., Il'ina, A.P., Monastyrnaya, M.M. et al. Biologically Active Polypeptides and Hydrolytic Enzymes in Sea Anemones of Northern Temperate Waters. Russian Journal of Marine Biology 29, 161–166 (2003). https://doi.org/10.1023/A:1024668732346

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