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Engineering Dictyostelium discoideum myosin II for the introduction of site-specific fluorescence probes

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Abstract

Dictyostelium discoideum is a useful host for the production of constructs for the analysis of structure–function relationships of myosin. Here we describe the use of myosin II constructs containing a single tryptophan residue, at different locations, for probing events at the nucleotide binding site, the relay loop and the communication path between them. GFP fusions have also been expressed at the N- and C-termini of the myosin motor to provide sensitive probes of the actomyosin dissociation reaction in microscope-based kinetic assays. We report on the fluorescence anisotropy of these constructs in the context of their use as resonance energy transfer probes.

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Authors and Affiliations

  1. Department of Biochemistry, University of Leicester, Leicester, LE1 7RH, United Kingdom

    Stuart Wakelin, Paul B. Conibear, Robert J. Woolley, David N. Floyd & Clive R. Bagshaw

  2. Department of Biochemistry, Eötvös Loránd University, H-1117, Budapest, Pázmány Péter sétány 1/C, Hungary

    Mihály Kovács & András Málnási-Csizmadia

Authors
  1. Stuart Wakelin
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  2. Paul B. Conibear
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  3. Robert J. Woolley
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  4. David N. Floyd
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  5. Clive R. Bagshaw
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  6. Mihály Kovács
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  7. András Málnási-Csizmadia
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Wakelin, S., Conibear, P.B., Woolley, R.J. et al. Engineering Dictyostelium discoideum myosin II for the introduction of site-specific fluorescence probes. J Muscle Res Cell Motil 23, 673–683 (2002). https://doi.org/10.1023/A:1024411208497

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