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Resonant Raman quantification of zeaxanthin production from Flavobacterium multivorum

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Abstract

Resonant Raman scattering was used as a novel, rapid, non-destructive optical technique to measure zeaxanthin levels in Flavobacterium multivorum ATCC 55238. Culture broth, after bacterial growth for 40 h, exhibited characteristic resonance Raman vibrational modes at 1159 cm−1 (C-C stretch) and 1525 cm−1 (C=C stretch) upon excitation at 488 nm. A striking correlation was observed between the carotenoid level as estimated by HPLC and by resonance Raman spectroscopy.

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References

  • Alcantara S, Sanchez S (1999) Influence of carbon and nitrogen sources on Flavobacterium growth and zeaxanthin biosynthesis. J. Ind. Microbiol. Biotechnol. 23: 697–700.

    Google Scholar 

  • Bernstein PS, Yoshida MD, Katz NB, McClane RW, Gellermann W (1998) Raman detection of macular carotenoid pigments in intact human retina. Invest. Ophthalmol. Vis. Sci. 39: 2003–2011.

    Google Scholar 

  • Bernstein PS, Zhao DY, Wintch SW, Ermakov IV, McClane RW, Gellermann W (2002) Resonance Raman measurement of macular carotenoids in normal subjects and in age-related macular degeneration patients. Ophthalmology 109: 1780–1787.

    Google Scholar 

  • Dalterio RA, Nelson WH, Britt D, Sperry J, Purcell FJ (1986) A resonance Raman microprobe study of chromobacteria in water. Appl. Spectrosc. 40: 271–272.

    Google Scholar 

  • Gill D, lponen RG, Rimai L (1970) Resonance Raman scattering of laser radiation by vibrational modes of carotenoid pigment molecules in intact plant tissues. Nature 227: 743–744.

    Google Scholar 

  • Hadden WL, Watkins RH, Levy LW, Regalado E, Rivadeneira DM, van Breemen RB, Schwartz SJ (1999) Carotenoid composition of marigold (Tagetes erecta) flower extract used as nutritional supplement. J. Agric. Food Chem. 47: 4189–4194.

    Google Scholar 

  • Handelman GJ, Nightingale ZD, Lichtenstein AH, Schaefer EJ, Blumberg JB (1999) Lutein and zeaxanthin concentrations in plasma after dietary supplementation with egg yolk. Am. J. Clin. Nutr. 70: 247–251.

    Google Scholar 

  • Hari RPK, Patel T, Martin A (1992) A new strain of Rhodotorula rubra isolated from yogurt. J. Ind. Microbiol. 11: 43–51.

    Google Scholar 

  • Humphries JM, Khachik F (2003) Distribution of lutein, zeaxanthin, and related geometrical isomers in fruit, vegetables, wheat, and pasta products. J. Agric. Food Chem. 51: 1322–1327.

    Google Scholar 

  • Jin E, Feth B, Melis A (2003) A mutant of the green alga Dunaliella salina constitutively accumulates zeaxanthin under all growth conditions. Biotechnol. Bioeng. 81: 115–124.

    Google Scholar 

  • Johnson EA, Schroeder WA (1996) Microbial carotenoids. Adv. Biochem. Eng. Biotechnol. 53: 119–178.

    Google Scholar 

  • Masetto A, Flores-Cotera LB, Diaz C, Langley E, Sanchez S (2001) Application of a complete factorial design for the production of zeaxanthin by Flavobacterium sp. J. Biosci. Bioeng. 92: 55–58.

    Google Scholar 

  • Moeller SM, Jacques PF, Blumberg JB (2000) The potential role of dietary xanthophylls in cataract and age-related macular degeneration. J. Am. Coll. Nutr. 5: 522–527.

    Google Scholar 

  • Nelis HJ, DeLeenheer AP (1991) Microbial sources of carotenoid pigments used in food and feeds. J. Appl. Bacteriol. 70: 181–191.

    Google Scholar 

  • Pasamontes L, Hug D, Tessier M, Hohmann HP, Schierle J, van Loon AP (1997) Isolation and characterization of the carotenoid biosynthesis genes of Flavobacterium sp. strain R1534. Gene 185: 35–41.

    Google Scholar 

  • Ruther A, Misawa N, Boger P, Sandmann G (1997) Production of zeaxanthin in Escherichia coli transformed with different carotenogenic plasmids. Appl. Microbiol. Biotechnol. 48: 162–167.

    Google Scholar 

  • Snodderly DM (1995) Evidence for protection against age-related macular degeneration by carotenoids and antioxidant vitamins. Am. J. Clin. Nutr. 62: 1448–1461.

    Google Scholar 

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Authors and Affiliations

  1. Department of Ophthalmology and Visual Sciences, University of Utah School of Medicine, 50 North Medical Drive, Moran Eye Center, Salt Lake City, UT, 84132, USA

    Prakash Bhosale & Paul S. Bernstein

  2. Department of Physics and Dixon Laser Institute, University of Utah, Salt Lake City, UT, 84112, USA

    Igor V. Ermakov, Maia R. Ermakova & Werner Gellermann

Authors
  1. Prakash Bhosale
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  2. Igor V. Ermakov
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  3. Maia R. Ermakova
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  4. Werner Gellermann
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  5. Paul S. Bernstein
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Correspondence to Paul S. Bernstein.

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Bhosale, P., Ermakov, I.V., Ermakova, M.R. et al. Resonant Raman quantification of zeaxanthin production from Flavobacterium multivorum . Biotechnology Letters 25, 1007–1011 (2003). https://doi.org/10.1023/A:1024199213519

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  • DOI: https://doi.org/10.1023/A:1024199213519

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