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Laser-assisted microinjection into targeted animal cells

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Abstract

A pulsed (17 nanoseconds) Nd:YAG laser (1064 nm) was used to inject impermeable dyes (propidium iodide andiodide and merocyanine 540) and a plasmid (pEGFP-N1) encoding green fluorescent protein (GFP) into human breast adenocarcinoma cells (MCF-7). The cell membrane integrity and viability were fully preserved in this laser-assisted transfer.

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Authors and Affiliations

  1. Biomedical Applications Section Center for Advanced Technology, Indore, 452013, India

    Samarendra K. Mohanty, Mrinalini Sharma & Pradeep K. Gupta

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  1. Samarendra K. Mohanty
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  2. Mrinalini Sharma
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  3. Pradeep K. Gupta
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Correspondence to Pradeep K. Gupta.

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Mohanty, S.K., Sharma, M. & Gupta, P.K. Laser-assisted microinjection into targeted animal cells. Biotechnology Letters 25, 895–899 (2003). https://doi.org/10.1023/A:1024038609045

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  • DOI: https://doi.org/10.1023/A:1024038609045

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