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Evidence for stable transformation of Pseudomonas aeruginosa with a pUC-based plasmid

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Abstract

Pseudomonas aeruginosa was transformed with pUC8:16, a pUC-based plasmid bearing the gene (vgb) encoding Vitreoscilla (bacterial) hemoglobin (VHb). Transformation was initially indicated by an increase in ampicillin resistance from 1500 to 2500 mg l−1. Presence of the plasmid in P. aeruginosa was confirmed by amplification of a portion of vgb from and detection of VHb in the transformant but not the untransformed host. Southern blot analysis further indicated that pUC8:16 existed as an autonomous plasmid rather than integrated into the chromosome of the P. aeruginosa transformant.

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Author notes

  1. Yongsoon Kim

    Present address: Department of Genetics, Yale University School of Medicine, New Haven, CT , 06520, USA

Authors and Affiliations

  1. Department of Genetics, Yale University School of Medicine, New Haven, CT , 06520, USA

    Benjamin C. Stark & Dale A. Webster

Authors
  1. Benjamin C. Stark
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  2. Yongsoon Kim
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  3. Dale A. Webster
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Correspondence to Benjamin C. Stark.

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Stark, B.C., Kim, Y. & Webster, D.A. Evidence for stable transformation of Pseudomonas aeruginosa with a pUC-based plasmid. Biotechnology Letters 25, 959–962 (2003). https://doi.org/10.1023/A:1024036319890

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  • DOI: https://doi.org/10.1023/A:1024036319890

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