Skip to main content
Log in

An In vitro Evaluation of Some Unconventional Ruminant Feeds in Terms of the Organic Matter Digestibility, Energy and Microbial Biomass

  • Published:
Tropical Animal Health and Production Aims and scope Submit manuscript

Abstract

In vitro organic matter apparent digestibility (IVOMAD), true digestibility (IVOMTD), metabolizable energy (ME), net energy lactation (NEL), microbial nitrogen (MN) and synthesis of microbial biomass (MBM) were estimated to predict the nutritive values of some agricultural by-products, drought-tolerant range plants and browses. The relationships between in vitro gas production (GP), and true or apparent digestibility, MN and MBM were studied utilizing an in vitro incubation technique. The values of IVOMAD, IVOMTD, ME, NEL, GP, MBM and MN varied with the studied experimental materials. The true fermentation of the outside part of Atriplex leucoclada produced a higher volume of gas than the middle or the inside parts, and this was associated with an increase in the values of IVOMAD, IVOMTD, ME and NEL. However, screening off the wood from olive cake to obtain olive cake pulp increased the IVOMAD, IVOMTD, ME, NEL and the volume of gas production from the true fermented material. One ml of gas was generated from the true degradation of 5 mg of wheat straw, Moringa oleifera, Alhagi camelorum, Eucaliptus camaldulensis and A. leucoclada, from 11 mg of Prosopsis stephaniana and olive cake pulp, and from 20 mg of olive cake or olive cake wood. The amount of MN or MBM produced from 100 mg of truly fermented organic matter depended on the kind of the fermented material and amounted to 0.7–2.9 mg or 8–34 mg, respectively. Crude fibre was negatively correlated to IVOMAD, IVOMTD, ME and NEL. Gas production was positively correlated to IVOMAD and IVOMTD but negatively correlated to MBM and MN.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

REFERENCES

  • Aderibigbe, A.O., Johnson, C.O.L.E., Makkar, H.P.S., Becker, K. and Foidl, N., 1997. Chemical composition and effect of heat on organic matter-and nitrogen-degradability and somem antinutritional components of Jatropha meal. Animal Feed Science and Technology, 67, 223-243

    Google Scholar 

  • Al-Masri, M.R., 1998. Yield and nutritive value of vetch (Vicia sativa)-barley (Hordeum vulgare) forage under different harvesting regimens. Tropical Grasslands, 32, 201-206

    Google Scholar 

  • Al-Masri, M.R., 1999. In-vitro digestible energy of some agricultural residues as influenced by gamma irradiation and sodium hydroxide. Applied Radiation and Isotopes, 50, 295-301

    Google Scholar 

  • Al-Masri, M.R., 2001. Changes in biogas production due to different ratios of animal and agricultural wastes. Bioresource Technology, 77, 97-100

    Google Scholar 

  • Al-Masri, M.R. and Guenther, K.D., 1995. The effect of gamma irradiation on in vitro digestible energy of some agricultural residues. Das Wirtschaftseigene Futter, 41, 61-68

    Google Scholar 

  • Al-Masri, M.R. and Zarkawi, M., 1994. Effects of gamma irradiation on chemical compositions of some agricultural residues. Radiation Physics and Chemistry, 43, 257-260

    Google Scholar 

  • Beever, D.E., 1993. Ruminal animal production from forages — present position and future opportunities. In: M.J. Baker (ed.), Grassland For Our World, (SIR Publishing), 158

  • Bischof, F., 1978. Common weeds from Iran, Turkey, the Near East and North Africa, (Deutsche Gesellschaft für Technische Zusammenarbeit, GTZ, GmbH, Germany)

    Google Scholar 

  • Blümmel, M., 1994. Relationship between kinetics of stover fermentation as described by the Hohenheim in vitro gass production test and voluntary feed intake of 54 cereal stovers, (Dissertation, Universität Hohenheim, Germany)

    Google Scholar 

  • Blümmel, M. and Becker, K., 1997. The degradability characteristics of fifty-four roughages and roughage neutral-detergent fibres, as described by in vitro gas production and their relationship to voluntary feed intake. British Journal of Nutrition, 77, 757-768

    Google Scholar 

  • Blümmel, M. and Ørskov, E.R., 1993. Comparison of in vitro gas production and nylon bag degradability of roughages in predicting feed intake in cattle. Animal Feed Science and Technology, 40, 109-119

    Google Scholar 

  • Blümmel, M., Makkar, H.P.S. and Becker, K., 1997a. In vitro gas production: a technique revisited. Journal of Animal Physiology and Animal Nutrition, 77, 24-34

    Google Scholar 

  • Blümmel, M., Steingass, H. and Becker, K., 1997b. The relationship between in vitro gas production, in vitro microbial biomass yield and 15N incorporation and its implications for the prediction of voluntary feed intake of roughages. British Journal of Nutrition, 77, 911-921

    Google Scholar 

  • Cano, A.L.M., Plumbly, R.A. and Hylands, P.J., 1989. Purification and partial characterization of the hemagglutination from seeds of Jatropha curcas. Journal of Food Biochemistry, 13, 1-20

    Google Scholar 

  • Close, W. and Menke, K.H., 1986. Selected Topics in Animal Nutrition, A manual prepared for the 3rd Hohenheim course on animal nutrition in the tropics and semi-tropics, 2nd edn, (University of Hohenheim, Stuttgart, Germany)

    Google Scholar 

  • Coenen, G., 1988. In vitro-Untersuchungen mit dem RUSITEC-System zum Einfluss unterschiedlicher Feet-Stärke-Kombinationen auf verschiedene Fermentationsparameter von Pansenmikroben, (Dissertation, Georg-August-Universität, Göttingen, Germany)

    Google Scholar 

  • Counotte, G.H.M. and Prins, R.A., 1979. Regulation of rumen lactate metabolism and the role of lactic acid in nutritional disorders of ruminants. Veterinary Science Communications, 2, 277-303

    Google Scholar 

  • Czerkawski, J.W., 1986. An Introduction to Rumen Studies, (Pergamon Press, Oxford)

    Google Scholar 

  • Goering, H.K. and Van Soest, P.J., 1970. Forage Fibre Analysis (Apparatus, Reagents, Procedures and Some Applications), Agriculture Handbook no. 379, (Agricultural Research Service, United States Department of Agriculture, Washington, USA)

    Google Scholar 

  • Gomez, C.D., Al-Masri, M.R., Steinberg, W. and Abel, Hj., 1998. Effect of varying hay/barley proportions on microbial biotin metabolism in the rumen-stimulating-technique RUSITEC. Proceedings of the Society of Nutrition Physiology, vol. 7, (DLG-Verlag, Germany)

    Google Scholar 

  • Heller, J., 1996. Physic Nut Jatropha curcas L. Promoting the Conservation and Use of Underutilized and Neglected Crops, vol. 1, (Institute of Plant Genetics and Crop Plant Research (Gatersleben) and International Plant Genetic Resources Institute, Rome)

    Google Scholar 

  • Hillman, H.K., Newbold, C.J. and Stewart, C.S., 1993. The contribution of bacteria and protozoa to ruminal forage fermentation in vitro, as determined by microbial gas production. Animal Feed Science and Technology, 42, 193-208

    Google Scholar 

  • Jimenez, L., Perez, L, de la Torre, M.J. and Garcia, J.C., 1999. The effect of processing variables on the soda pulping of olive tree wood. Bioresource Technology, 69, 95-102

    Google Scholar 

  • Khazaal, K. and Ørskov, E.R., 1994. The in vitro gas production technique: an investigation on its potential use with polyvinylpyrrolidone for the assessment of phenolic related antinutritive factors in browse. Animal Feed Science and Technology, 47, 305-320

    Google Scholar 

  • Kristensen, V.F. and Weisbjerg, M.R., 1990. New approach to feed evaluation for ruminants. A note to the meeting of the Nordic Working group on feed evaluation in Iceland, 12–13 August, 1990, Rejkjavik, Iceland

  • Leng, R.A., 1993. Quantitative ruminant nutrition — a green science. Australian Journal of Agricultural Research, 44, 363-380

    Google Scholar 

  • Makkar, H.P.S. and Becker, K., 1996. Nutritional value and antinutritional components of whole and ethanol extracted Moringa oleifera leaves. Animal Feed Science and Technology, 63, 211-228

    Google Scholar 

  • Menke, K.H. and Steingass, H., 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research Development, 28, 7-55

    Google Scholar 

  • Menke, K.H., Raab, L., Salewski, A., Steingass, H., Fritz, D. and Schneider, W., 1979. The estimation of the digestibility and metabolizable energy content of ruminant feedstuffs from the gas production when they are incubated with rumen liquor in vitro. Journal of Agricultural Science, 93, 217-222

    Google Scholar 

  • Minson, D.J., 1982. Effect of chemical composition of feed digestibility and metabolizable energy. Nutrition Abstract Review, Series B, 52, 592-615

    Google Scholar 

  • Mouterde, P., 1966. Nouvelle Flore du Liban et de la Syrie, Tome Premier Atlas, (Editions de l'Imprimerie Catholique, Beyrouth, Liban)

    Google Scholar 

  • Naumann, C. and Bassler, R., 1976. Die chemische Untersuchung von Futtermitteln, Methodenbuch Band III, 6-2, (Neumann-Neudamm, Berlin), 1

    Google Scholar 

  • Nautiyal, B.P. and Venhataraman, K.G., 1987. Moringa (drumstick) — an ideal tree of social forestry: growing conditions and uses — Part I. Myforest, 23, 53

    Google Scholar 

  • Nsahlai, I.V., Siaw, D.E.K.A. and Osuji, P.O., 1994. The relationship between gas production and chemical composition of 23 browses of the genus Sesbania. Journal of the Science of Food and Agriculture, 65, 13-20

    Google Scholar 

  • Popple, D.P., Minson, D.J. and Ternouth, J.H., 1981. Studies of cattle and sheep eating leaf and stem fractions of grasses. II — Factors controlling the retention of feed in the reticulo-rumen. Australian Journal of Agricultural Research, 32, 109-121

    Google Scholar 

  • Russell, J.B., O'Connor, J.D., Fox, D.G., Van Soest, P.J. and Sniffen, C.J., 1992. A net carbohydrate and protein system for evaluating cattle diets: 1. Ruminal fermentation. Journal of Animal Science, 70, 3551-3561

    Google Scholar 

  • Sniffen, C.J., O'Connor, J.D., Van Soest, P.J., Fox, D.G. and Russell, J.B., 1992. A net carbohydrate and protein system for evaluating cattle diets: 2. Carbohydrate and protein availability. Journal of Animal Science, 70, 3562-3577

    Google Scholar 

  • Steingass, H. and Menke, K., 1986. Schätzung des energetischen Futterwertes aus der in vitro mit Pansensaft bestimmten Gasbildung und der chemischen Analyse. 1. Untersuchungen zur Methode. Tierernährung, 14, 251-270

    Google Scholar 

  • Ushida, K., Jouany, J.P. and Demeyer, D.I., 1991. Rumen microogranisms and their nutrition. In: E.R. Ørskov (ed.), Protein Nutrition in Ruminants, 2nd edn, (Academic Press, London), 20-42

    Google Scholar 

  • Van Soest, P.J. and Robertson, J.B., 1985. Analyses of Forages and Fibrous Foods, A Laboratory Manual for Animal Science, no. 612, Cornell University, Ithaca)

    Google Scholar 

  • Zoght, M.F., 1978. Sand Dunes (Fixation, Afforestation, Exploitation), (The Arab Center for the Studies of Arab Zones and Dry Lands. ACSAD/R10/77, Damascus, Syria)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Al-Masri, M. An In vitro Evaluation of Some Unconventional Ruminant Feeds in Terms of the Organic Matter Digestibility, Energy and Microbial Biomass. Tropical Animal Health and Production 35, 155–167 (2003). https://doi.org/10.1023/A:1022877603010

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1022877603010

Navigation